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Characterization and use of a recombinant retroviral system for the analysis of drug resistant HIV.

Abstract：

:A recombinant retroviral system was used for the analysis of early HIV breakthrough infection in the presence of antiviral drugs. The use of replication-defective HIV allowed a quantitative analysis of a single cycle of infection. This report characterizes this recombinant HIV system and demonstrates it's validity in comparison to standard assays. It is demonstrated that the protease inhibitor XM323 inhibits both early and late events in the HIV life-cycle, while dextran sulphate inhibits only early events. In addition, it is shown that this system can be used for detecting and quantitating drug resistant HIV. Thus, the use of this system may provide both novel information about the stage of the viral life-cycle inhibited and a preliminary assessment of the mechanism(s) responsible for breakthrough infection in the presence of antiretroviral drugs.

journal_name

J Virol Methods

journal_title

Journal of virological methods

authors

Medina DJ,Tung PP,Nelson CJ,Sathya B,Casareale D,Strair RK

doi

10.1016/s0166-0934(97)00212-7

subject

Has Abstract

pub_date

1998-04-01 00:00:00

pages

169-76

issue

2

eissn

0166-0934

issn

1879-0984

pii

S0166093497002127

journal_volume

71

pub_type

杂志文章

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