Abstract：
:A virus (PBCV) from the symbiotic Chlorella-like algae of Paramecium bursaria has been characterized by sedimentation field flow fractionation (SdFFF). The method yields effective molecular weight M' (molecular weight X buoyancy factor) with a precision of better than 5% under a variety of experimental conditions. The value for M' is used to calculate true molecular weight values through combination with density values; densities were obtained from outside sources, from ultrasonic densitometry, and from sedimentation coefficients determined independently. The actual molecular weight is also determined through SdFFF analysis of the virus in carriers of different densities. Values for the particle diameter are obtained from SdFFF zone broadening studies. The various SdFFF characterization results are compared with molecular weight, particle size, and density values determined by conventional methods.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Yonker CR,Caldwell KD,Giddings JC,Van Etten JLdoi
10.1016/0166-0934(85)90038-2subject
Has Abstractpub_date
1985-06-01 00:00:00pages
145-60issue
2eissn
0166-0934issn
1879-0984pii
0166-0934(85)90038-2journal_volume
11pub_type
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