当前位置： SCI文献检索 > JOURNAL OF VIROLOGICAL METHODS期刊下所有文献 > Simple microwave and thermal cycler boiling methods for preparation of cervicovaginal lavage cell samples prior to PCR for human papillomavirus detection.

Simple microwave and thermal cycler boiling methods for preparation of cervicovaginal lavage cell samples prior to PCR for human papillomavirus detection.

Abstract：

:Sample preparation is an important step in the detection of viral DNA by the polymerase chain reaction (PCR) technique. The method used should achieve release of cellular DNA with the minimum of manipulation steps so as to reduce the possibility of contamination. The present report demonstrates that either microwaving or 20 min of boiling in the heating block of a thermal cycler lead to satisfactory results in the detection by PCR of human papillomavirus in cervicovaginal epithelial cell specimens obtained by lavage. Since each of these methods uses only one step the possibility of contamination is greatly reduced compared to the widely used proteins K/nonionic detergent extraction procedure.

journal_name

J Virol Methods

journal_title

Journal of virological methods

authors

Lou YK,Qin H,Molodysky E,Morris BJ

doi

10.1016/0166-0934(93)90009-g

subject

Has Abstract

pub_date

1993-09-01 00:00:00

pages

77-81

issue

1

eissn

0166-0934

issn

1879-0984

pii

0166-0934(93)90009-G

journal_volume

44

pub_type

杂志文章

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