Abstract:
:Dengue virus infection is a major public health problem throughout tropical countries. In endemic areas, dengue hemorrhagic fever (DHF) or dengue shock syndrome (DSS) are common complications resulting in death. However, serological confirmation of dengue-related illness is often complicated and time-consuming. Detection of dengue viruses in clinical or field samples usually depends on virus isolation in susceptible cell lines or in mosquitoes, followed by viral protein identification using polyclonal or monoclonal antibodies. The increasing incidence of dengue virus infections has prompted increased efforts to develop rapid and reliable diagnostic techniques. A simple microplate hybridization method was developed for identification of viral RNA. Microplate hybridization is simpler than enzyme-linked immunosorbent assay and has several advantages over the conventional dot-blot hybridization method: (1) radioisotopes are not necessary; (2) synthetic oligonucleotide for the probe is not needed; (3) the time required for washing of the solid phase is greatly reduced; and (4) baking is eliminated. The results show that this procedure is sensitive, rapid and easy to perform.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Ruiz BH,Zamora MP,Liu Sdoi
10.1016/0166-0934(95)00026-qsubject
Has Abstractpub_date
1995-08-01 00:00:00pages
97-108issue
2-3eissn
0166-0934issn
1879-0984pii
0166-0934(95)00026-Qjournal_volume
54pub_type
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journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/j.jviromet.2012.02.008
更新日期:2012-05-01 00:00:00
abstract:BACKGROUND:Polyomavirus BK (BKV) may cause nephropathy in renal transplant recipients and hemorrhagic cystitis in bone marrow recipients. We developed real-time PCRs (RT-PCR) to determine easily and rapidly the different BKV genotypes (BKGT) (I-IV). METHODS:On the VP1 gene a duplex of RT-PCRs was developed and validat...
journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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doi:10.1016/0166-0934(90)90133-z
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abstract::Viruses belonging to the Flaviviridae family are found and distributed in most of the tropical and sub-tropical regions of the world. The genus has more than 56 members, most of which cause clinical symptoms in humans. The clinical diagnosis of dengue requires laboratory confirmation because of the similarity of sympt...
journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/j.jviromet.2012.08.006
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journal_title:Journal of virological methods
pub_type: 杂志文章
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journal_title:Journal of virological methods
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doi:10.1016/j.jviromet.2009.02.022
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abstract::Citrus leaf blotch virus (CLBV) is a type member of the genus Citrivirus belonging to Betaflexiviridae. In this study, a reverse transcription loop-mediated isothermal amplification (RT-LAMP) method was developed to detect CLBV; this technology has been widely used in the detection of various plant pathogenic microorg...
journal_title:Journal of virological methods
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doi:10.1016/j.jviromet.2019.05.009
更新日期:2019-08-01 00:00:00
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journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/0166-0934(81)90050-1
更新日期:1981-10-01 00:00:00
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journal_title:Journal of virological methods
pub_type: 杂志文章
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更新日期:2018-11-01 00:00:00
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journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/0166-0934(87)90055-3
更新日期:1987-02-01 00:00:00
abstract::A transfer vector was constructed in which the neomycin resistance (neo) gene was under the control of a copy of Autographa californica nuclear polyhedrosis virus (AcMNPV) IE1 gene promoter at the p10 locus. After cotransfection of Spodoptera frugiperda (Sf9) cells with the transfer vector and infectious AcMNPV DNA, t...
journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/0166-0934(96)02112-x
更新日期:1996-10-01 00:00:00
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journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/0166-0934(84)90009-0
更新日期:1984-10-01 00:00:00
abstract::Porcine endogenous retroviruses (PERV) represent a risk for xenotransplantation using pig cells, tissues or organs. PERV-A and PERV-B are present in the genome of all pigs and both infect human cells in vitro. PERV-C infects only pig cells and it is integrated in the genome of most, but not all pigs. Recombinants betw...
journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/j.jviromet.2011.04.017
更新日期:2011-07-01 00:00:00
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journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/j.jviromet.2010.07.024
更新日期:2010-11-01 00:00:00
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journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/j.jviromet.2016.09.002
更新日期:2016-11-01 00:00:00
abstract::Antigenic differences among cowpea severe mosaic virus (CPSMV) isolates were clearly reflected in signature analysis employing a panel of seven well-characterized, monoclonal antibodies. Separate binding curves were generated by reacting serial dilutions of extracts from infected plant tissue containing each antigen s...
journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/0166-0934(93)90039-t
更新日期:1993-05-01 00:00:00
abstract::As a prelude to the development of nucleic acid probes specific for non-A, non-B hepatitis virus(es) (NANBV), plasmas with alanine aminotransferase levels greater than or equal to 110 IU were assayed for DNA by a radioimmunoassay. Approximately 50% of such plasmas are expected to contain NANBV. One-hundred and seventy...
journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/0166-0934(84)90042-9
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journal_title:Journal of virological methods
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doi:10.1016/0166-0934(94)90053-1
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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更新日期:1992-02-01 00:00:00
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journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/0166-0934(90)90099-2
更新日期:1990-03-01 00:00:00
abstract::Photobiotin was used to prepare biotinylated, nonradioactive nucleic acid probes for the detection of the RNA of barley yellow dwarf virus (BYDV) in plant extracts. A 1.7-kb cDNA of the PAV isolate of BYDV in the plasmid pUC8 vector was biotinylated and then used intact or as sonicated double-stranded DNA fragments. S...
journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/0166-0934(87)90007-3
更新日期:1987-06-01 00:00:00
abstract::Three serologic methods for the detection of antibodies to bovine leukemia virus (BLV) were compared using the sera of 140 dairy cows. A widely used commercial agarose immunodiffusion screening assay and a commercial antibody capture enzyme immunosorbent assay were compared for sensitivity and specificity with immunob...
journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/s0166-0934(02)00040-x
更新日期:2002-06-01 00:00:00
abstract::Three tests were compared to diagnose herpes virus infection: electron microscopy (EM), viral culture (VC) and cytodiagnosis (Tzanck smear). The study comprised 67 patients with skin or mucous membrane lesions suggestive of herpes simplex virus (HSV) infection. The sensitivity of EM increased 25% after virus concentra...
journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/0166-0934(91)90106-a
更新日期:1991-10-01 00:00:00
abstract::Foot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed livestock which has a drastic economic impact for affected countries. Although FMDV is distributed worldwide, many regional programs have been effective eradicating this agent. In Argentina, as in many other regions of South America, the comb...
journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/j.jviromet.2011.09.011
更新日期:2011-12-01 00:00:00
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journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/j.jviromet.2015.04.023
更新日期:2015-09-01 00:00:00
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journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/j.jviromet.2011.06.016
更新日期:2011-10-01 00:00:00
abstract::A simple procedure is described for reproducible detection of double stranded (ds) RNAs in leafroll infected grapevines. The procedure involves the extraction of tissues by a medium which preferentially yields dsRNA. The RNA is purified by CF11 cellulose chromatography and gel electrophoresis. The dsRNAs varied in siz...
journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/0166-0934(91)90170-5
更新日期:1991-02-01 00:00:00
abstract::A simple, and sensitive method for the detection of human papillomavirus (HPV) 16 E6/E7 transcripts in RNA purified from formalin acetic alcohol (FAA)-fixed, paraffin embedded cervical cancer (CaCx) tissue is described. The entire procedure, including polymerase chain reaction (PCR) amplification of cDNA obtained from...
journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/0166-0934(91)90072-8
更新日期:1991-12-01 00:00:00