Abstract:
:The reverse transcriptase of the sheep lentivirus visna/maedi virus has been characterised. Optima for magnesium ion concentration (5-10 mM), potassium ion concentration (150 mM) and pH (8.25) for this enzyme are very similar to those previously described for the human immunodeficiency viruses. The assay used for this work makes use of a cell harvester to speed up the processing of multiple samples. It is small scale, requiring 15 microliters of sample, is rapid, and is able to detect virus at titres below 10(3)/ml. Harvesting the assay onto either DEAE paper or using TCA and glass fibre mats make it suitable for use with either tissue culture media or infected cell lysates, but not with body fluids. It has been used to detect cell-associated reverse transcriptase in choroid plexus cells within 36 h of visna infection.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Sargan DR,Watt NJ,McConnell Idoi
10.1016/0166-0934(92)90144-3subject
Has Abstractpub_date
1992-02-01 00:00:00pages
129-40issue
2eissn
0166-0934issn
1879-0984pii
0166-0934(92)90144-3journal_volume
36pub_type
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