当前位置： SCI文献检索 > JOURNAL OF VIROLOGICAL METHODS期刊下所有文献 > Specificity of two HIV screening tests detecting simultaneously HIV-1 p24 antigen and antibodies to HIV-1 and -2.

Specificity of two HIV screening tests detecting simultaneously HIV-1 p24 antigen and antibodies to HIV-1 and -2.

Abstract：

:This study aimed at assessing the specificity of the Elecsys® HIV combi PT in comparison to the ARCHITECT® HIV Ag/Ab Combo. With both of these assays, 3997 unselected sera from patients of a tertiary health care centre in Basel, Switzerland, were screened for HIV. Reactive sera were reanalysed on the VIDAS® HIV Duo Ultra to identify false-reactive specimens prior to confirmation by quantitative PCR and line immunoassay. The Elecsys® compared to the ARCHITECT® shows a similar specificity (99.7% versus 99.8%) but a slightly lower positive predictive value (71.8% versus 80%). Samples tested with a cut-off index (COI) between 0.91 and 4.85 (cut-off <0.9) with the Elecsys® and with a signal to cut-off index (S/CO) between 1.09 and 12.49 (cut-off <1.0) with the ARCHITECT® were false-reactive. There was no false-reactive result with the VIDAS®. Of the false-reactive samples, 66.7% could be related to patient-specific underlying conditions. The HIV two-tiered diagnostic algorithm proposed in this work improved the positive predictive values of the Elecsys® or ARCHITECT® to 100% when the results of the VIDAS® were included. Values just above the cut-off are highly suspicious to be false-reactive and high COI or S/CO ratios are associated with true positivity.

journal_name

J Virol Methods

journal_title

Journal of virological methods

authors

Blaich A,Buser A,Stöckle M,Gehringer C,Hirsch HH,Battegay M,Klimkait T,Frei R

doi

10.1016/j.jviromet.2017.09.005

subject

Has Abstract

pub_date

2017-11-01 00:00:00

pages

143-146

eissn

0166-0934

issn

1879-0984

pii

S0166-0934(17)30420-2

journal_volume

249

pub_type

杂志文章

相关文献

JOURNAL OF VIROLOGICAL METHODS文献大全
  • A rapid screening assay for detecting individual RNA species in field isolates of rotaviruses.

    abstract：:A method is described that allows the rapid screening of field isolates of rotavirus for the detection of specific viral RNA segments. Cloned cDNA copies of viral genomic RNAs are employed for detection in the assay which makes use of the dot-hybridization technique of Thomas (1980). The assay developed was shown to b...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(84)90009-0

    authors： Pedley S,McCrae MA

    更新日期：1984-10-01 00:00:00

  • Detection of pandemic influenza A/H1N1/2009 virus by real-time reverse transcription polymerase chain reaction.

    abstract：:The first case of pandemic influenza A/H1N1/2009 infection was reported in Mexico in mid-April, 2009, and to date the new H1N1 virus has spread to over 160 countries. Therefore, it is important to obtain reliable epidemiological data on the spread of this virus by novel molecular methods can be developed in detection ...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2009.11.033

    authors： Wu W,Kang X,Bai Z,Liu L,Li J,Wu X,Sun H,Hu T,Yang M,Wang P,Liu L,Yang Y,Di B,Chen W

    更新日期：2010-05-01 00:00:00

  • Confirmation of a new conserved linear epitope of Lyssavirus nucleoprotein.

    abstract：:Bioinformatics analysis was used to predict potential epitopes of Lyssavirus nucleoprotein and highlighted some distinct differences in the quantity and localization of the epitopes disclosed by epitope analysis of monoclonal antibodies against Lyssavirus nucleoprotein. Bioinformatics analysis showed that the domain c...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2012.02.008

    authors： Xinjun L,Xuejun M,Lihua W,Hao L,Xinxin S,Pengcheng Y,Qing T,Guodong L

    更新日期：2012-05-01 00:00:00

  • Demonstration of rabbit haemorrhagic disease virus antigen by Staphylococcus protein A coagglutination test.

    abstract：:The Staphylococcus protein A coagglutination test (Sp A COAT) was developed for the diagnosis of rabbit haemorrhagic disease (RHD). Liver, spleen, lungs and kidneys from 176 rabbits dead from viral haemorrhagic disease as well as the same organs from 64 healthy animals were examined by Sp A COAT and haemaglutination t...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(95)01914-6

    authors： Peshev R,Alexandrov M,Ivanov Y,Gogov Y

    更新日期：1996-03-01 00:00:00

  • A newly developed immunofluorescence assay for simultaneous detection of antibodies to human immunodeficiency virus type 1 and type 2.

    abstract：:Immunofluorescence assays (IFA) that simultaneously distinguish between antibodies against closely related human immunodeficiency virus type 1 (HIV-1) and type 2 (HIV-2) infections have not been readily available. Serum specimens from 95 HIV-1-infected, 26 HIV-2-infected and 3 HIV-1/HIV-2 dually infected individuals a...

    journal_title：Journal of virological methods

    pub_type： 临床试验,杂志文章

    doi：10.1016/0166-0934(94)00107-r

    authors： Tanabe-Tochikura A,Ang Singh MT,Tsuchie H,Zhang J,Paladin FJ,Kurimura T

    更新日期：1995-04-01 00:00:00

  • Cellufine sulfate column chromatography as a simple, rapid, and effective method to purify dengue virus.

    abstract：:Conventional method to purify/concentrate dengue virus (DENV) is time-consuming with low virus recovery yield. Herein, we applied cellufine sulfate column chromatography to purify/concentrate DENV based on the mimicry between heparan sulfate and DENV envelope protein. Comparative analysis demonstrated that this new me...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2016.04.023

    authors： Kanlaya R,Thongboonkerd V

    更新日期：2016-08-01 00:00:00

  • Generation and characterization of an H5N1 avian influenza virus hemagglutinin glycoprotein pseudotyped lentivirus.

    abstract：:An H5N1 avian influenza virus (AIV) hemagglutinin (HA) protein pseudotyped lentivirus, HIV/H5-HA, was generated, characterized in vitro and evaluated for its ability to induce protective immunity against virulent wild type AIV in mice. The HIV/H5-HA virus was able to infect 293T, BHK, Vero, PK-15, MDCK cells but not I...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2008.08.016

    authors： Zhang S,Xiao L,Zhou H,Yu Z,Chen H,Guo A,Jin M

    更新日期：2008-12-01 00:00:00

  • A cost-effective algorithm for the diagnosis of Hepatitis C virus infection and prediction of HCV viremia in Cameroon.

    abstract：:Conventional tests for antibody to Hepatitis C virus (HCV) and HCV RNA require considerable time before results are available, remain very expensive and are not adapted to many sub-Saharan African countries where HCV is endemic. The aim of this study was to evaluate the accuracy of an algorithm consisting of two HCV r...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2005.11.001

    authors： Njouom R,Tejiokem MC,Zanga MC,Pouillot R,Ayouba A,Pasquier C,Nerrienet E

    更新日期：2006-05-01 00:00:00

  • Generation of a DNA microarray for determination of E6 natural variants of human papillomavirus type 16.

    abstract：:Infection with high-risk types of human papillomavirus (HPV) is necessary for the development of cervical cancer. However, the majority of the HPV infections are efficiently cleared by the immune system and only a minority persist and induce the development of malignant lesions. Several studies provided evidence that ...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2004.02.018

    authors： Gemignani F,Landi S,Chabrier A,Smet A,Zehbe I,Canzian F,Tommasino M

    更新日期：2004-08-01 00:00:00

  • Evaluation of enzyme-linked immunosorbent assays: a method of data analysis.

    abstract：:Diagnostic tests are usually evaluated in terms of simple qualitative measures of sensitivity and specificity. When comparing different quantitative assays such as ELISAs, it is often more useful to deal with actual values (sample optical density/cut-off optical density ratio (OD ratio] rather than the qualitative rel...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(88)90087-0

    authors： Crofts N,Maskill W,Gust ID

    更新日期：1988-10-01 00:00:00

  • A PCR-restriction enzyme technique for determining dengue virus subgroups within serotypes.

    abstract：:The polymerase chain reaction (PCR) and restriction enzyme analysis were used to develop a rapid and simple procedure for identifying geographic subgroups of dengue virus within serotypes for epidemiologic investigations. The entire structural protein region of dengue viruses was amplified and the products were digest...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(94)90122-8

    authors： Vorndam V,Kuno G,Rosado N

    更新日期：1994-07-01 00:00:00

  • Development of a high-throughput human rhinovirus infectivity cell-based assay for identifying antiviral compounds.

    abstract：:Asthma and chronic obstructive pulmonary disease exacerbations are associated with human rhinovirus (HRV) lung infections for which there are no current effective antiviral therapies. To date, HRV infectivity of cells in vitro has been measured by a variety of biochemical and immunological methods. This paper describe...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2011.02.002

    authors： Phillips T,Jenkinson L,McCrae C,Thong B,Unitt J

    更新日期：2011-05-01 00:00:00

  • Inducible model to study negative strand RNA synthesis and assembly of hepatitis C virus from a full-length cDNA clone.

    abstract：:An inducible in vitro cell culture system was developed to assay HCV replication by direct biochemical means. A transcription plasmid containing a T7 promoter at the 5' end, full-length cDNA of the HCV genome, a ribozyme sequence from the antigenomic strand of hepatitis delta virus and a T7 terminator was prepared. To...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/s0166-0934(01)00278-6

    authors： Myung J,Khalap N,Kalkeri G,Garry R,Dash S

    更新日期：2001-05-01 00:00:00

  • Development and evaluation of a droplet digital PCR assay for the detection of fowl adenovirus serotypes 4 and 10 in attenuated vaccines.

    abstract：:In recent years, there has been an increase in reported cases of fowl adenovirus serotype 4 (FAdV-4) in chickens in China. The use of live attenuated vaccines contaminated with FAdV-4 has been proved to be one of the important causes of massive outbreaks of hydropericardium syndrome. To detect the contamination with F...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2018.09.005

    authors： Dong G,Meng F,Zhang Y,Cui Z,Lidan H,Chang S,Zhao P

    更新日期：2019-03-01 00:00:00

  • Optimization of allele-specific PCR using patient-specific HIV consensus sequences for primer design.

    abstract：:Allele-specific PCR based on subtype consensus sequences is a powerful technique for detecting low frequency drug resistant mutants in HIV-1 infected patients. However, this approach can be limited by genetic variation in the region complementary to the primers, leading to variability in allele detection. The goals of...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2009.11.025

    authors： Boltz VF,Maldarelli F,Martinson N,Morris L,McIntyre JA,Gray G,Hopley MJ,Kimura T,Mayers DL,Robinson P,Mellors JW,Coffin JM,Palmer SE

    更新日期：2010-03-01 00:00:00

  • Comparison of HIV-1 detection in plasma specimens and dried blood spots using the Roche COBAS Ampliscreen HIV-1 test in Kisumu, Kenya.

    abstract：:The World Health Organization recommends screening donor blood for HIV in centralized laboratories. This recommendation contributes to quality, but presents specimen transport challenges for resource-limited settings which may be relieved by using dried blood spots (DBS). In sub-Saharan Africa, most countries screen d...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2011.07.001

    authors： Okonji JA,Basavaraju SV,Mwangi J,Shiraishi RW,Odera M,Ouma K,Pitman JP,Marum LH,Ou CY,Zeh C

    更新日期：2012-01-01 00:00:00

  • An in-well direct lysis method for rapid detection of SARS-CoV-2 by real time RT-PCR in eSwab specimens.

    abstract：BACKGROUND:Diagnostic real time reverse transcription PCR (rRT-PCR) is usually done using nucleic acid (NA) purified from the sample. In the SARS-CoV-2 pandemic reagents and utensils for NA purification has been in short supply. This has generated interest in methods that eliminate the need for NA purification. OBJECT...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2021.114062

    authors： Jørgensen RL,Pedersen MS,Chauhan AS,Andreasson LM,Kristiansen GQ,Lisby JG,Rosenstierne MW,Schønning K

    更新日期：2021-01-08 00:00:00

  • Expression and purification of bioactive hemagglutinin protein of highly pathogenic avian influenza A (H5N1) in silkworm larvae.

    abstract：:The hemagglutinin (HA) of avian influenza viruses plays a very important role in the infection of host cells. In this study, the HA gene of the highly pathogenic avian influenza H5N1 virus was cloned and expressed in silkworm larvae. The expressed recombinant HA (rHA) was purified using fetuin-agarose chromatography a...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2013.08.040

    authors： Dong J,Harada M,Yoshida S,Kato Y,Murakawa A,Ogata M,Kato T,Usui T,Park EY

    更新日期：2013-12-01 00:00:00

  • DNA: DNA hybridization method for the diagnosis of hepatitis B infection.

    abstract：:Hepatitis B viral (HBV) DNA was detected in a hepatoma cell line which produces hepatitis B surface antigen (HBsAg) and in patients with acute hepatitis B. The serum of one patient with acute hepatitis B was found to be infectious when injected i.v. into a chimpanzee up to a dilution of 10(-8). Hepatitis B surface ant...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(84)90014-4

    authors： Feinman SV,Berris B,Guha A,Sooknanan R,Bradley DW,Bond WW,Maynard JE

    更新日期：1984-05-01 00:00:00

  • Paramyxovirus infections in ex vivo lung slice cultures of different host species.

    abstract：:Paramyxoviruses, including measles virus (MV), human metapneumovirus (HMPV), human respiratory syncytial virus (HRSV) and canine distemper virus (CDV), are transmitted via the respiratory route. Despite their close phylogenetic relationship, the pathogenesis of these viruses is very different. To study viral tropism a...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2013.06.016

    authors： Nguyen DT,de Vries RD,Ludlow M,van den Hoogen BG,Lemon K,van Amerongen G,Osterhaus AD,de Swart RL,Duprex WP

    更新日期：2013-10-01 00:00:00

  • Preservation of RNA and destruction of infectivity in microdissected brain tissues of Lewis rats infected with the Borna disease virus.

    abstract：:Laser microdissection combined with real-time RT-PCR presents an advanced tool to quantify particular RNA species in defined tissue areas. Dealing with infectious tissue samples increases the need to overcome the risk of infectivity and contamination during laser microdissection. Here, an useful method to control infe...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2006.03.024

    authors： Porombka D,Herzog S,Baumgärtner W,Herden C

    更新日期：2006-08-01 00:00:00

  • Improved detection of HSV by electron microscopy in clinical specimens using ultracentrifugation and colloidal gold immunoelectron microscopy: comparison with viral culture and cytodiagnosis.

    abstract：:Three tests were compared to diagnose herpes virus infection: electron microscopy (EM), viral culture (VC) and cytodiagnosis (Tzanck smear). The study comprised 67 patients with skin or mucous membrane lesions suggestive of herpes simplex virus (HSV) infection. The sensitivity of EM increased 25% after virus concentra...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(91)90106-a

    authors： Folkers E,Vreeswijk J,Oranje AP,Wagenaar F,Duivenvoorden JN

    更新日期：1991-10-01 00:00:00

  • Development of a versatile and stable internal control system for RT-qPCR assays.

    abstract：:RT-qPCR, an established method for the detection of RNA viruses, requires internal RNA controls for the correct interpretation of PCR results. Robust and versatile RT-PCR controls can be achieved for example by packaging RNA into a virus-derived protein shell. In this study a MS2-based internal control system was deve...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2014.07.028

    authors： Felder E,Wölfel R

    更新日期：2014-11-01 00:00:00

  • Real-time TaqMan RT-PCR for detection of maize chlorotic mottle virus in maize seeds.

    abstract：:Maize chlorotic mottle virus (MCMV) causes corn lethal necrosis disease, and can be transmitted through infected maize seeds. It remains a challenge to detect this virus in the seeds to prevent its introduction and infection. For this purpose, a real-time TaqMan RT-PCR procedure for efficient detection of MCMV was dev...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2010.11.002

    authors： Zhang Y,Zhao W,Li M,Chen H,Zhu S,Fan Z

    更新日期：2011-01-01 00:00:00

  • Development of a multiplex real-time RT-PCR that allows universal detection of influenza A viruses and simultaneous typing of influenza A/H1N1/2009 virus.

    abstract：:On June 11, 2009, the World Health Organization declared that the influenza A/H1N1/2009 virus had become the first influenza pandemic of the 21st century. Rapid detection and differentiation from seasonal and avian influenza would be beneficial for patient management and infection control. It was the aim of this study...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2009.10.006

    authors： Gunson R,Maclean A,Davies E,Bennett S,Miller R,Carman WF

    更新日期：2010-02-01 00:00:00

  • Detection of CMV in urine: comparison between DNA-DNA hybridization, virus isolation, and immunoelectron microscopy.

    abstract：:Rapid detection of CMV-DNA in urine specimens by dot-blot hybridization was compared to conventional virus isolation and to virus identification using solid-phase immunoelectron microscopy (SPIEM). To detect viral DNA, 32P-labeled EcoR1 J fragment of CMV-DNA was used as a probe in the hybridization assay. In addition,...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(87)90028-0

    authors： Vonsover A,Gotlieb-Stematsky T,Sayar Y,Bardov L,Manor Y,Siegal B

    更新日期：1987-05-01 00:00:00

  • Mass spectrometric identification and characterisation of the nucleocapsid protein of Menangle virus.

    abstract：:The recent emergence of novel viruses requires reliable methodology for their identification and confirmation both on a cellular and molecular level. Mass spectrometry offers a suitable approach for the identification and characterisation of viral proteins and its application is demonstrated in this study. Menangle vi...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/s0166-0934(01)00441-4

    authors： Shiell BJ,Beddome G,Michalski WP

    更新日期：2002-04-01 00:00:00

  • Multiplex real-time PCR for identification of canine parvovirus antigenic types.

    abstract：:Canine parvovirus (CPV) is an important disease causing gastroenteritis and/or haemorrhagic gastroenteritis in dogs. There are four antigenic types of CPV reported worldwide viz. CPV 2, CPV 2a, CPV 2b and CPV 2c. The diagnosis of CPV with the identification of the antigen type responsible remains problematic. In the p...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2016.02.013

    authors： Kaur G,Chandra M,Dwivedi PN,Narang D

    更新日期：2016-07-01 00:00:00

  • A novel HIV-1 reporter virus with a membrane-bound Gaussia princeps luciferase.

    abstract：:HIV-1 reporter viruses are a critical tool for investigating HIV-1 infection. By having a reporter gene incorporated into the HIV-1 genome, the expressed reporter protein acts as a specific tag, thus enabling specific detection of HIV-1 infected cells. Currently existing HIV-1 reporter viruses utilize reporters for th...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2012.03.028

    authors： Suree N,Koizumi N,Sahakyan A,Shimizu S,An DS

    更新日期：2012-07-01 00:00:00

  • Simultaneous detection of eight swine reproductive and respiratory pathogens using a novel GeXP analyser-based multiplex PCR assay.

    abstract：:A new high-throughput GenomeLab Gene Expression Profiler (GeXP) analyser-based multiplex PCR assay was developed for the detection of eight reproductive and respiratory pathogens in swine. The reproductive and respiratory pathogens include North American porcine reproductive and respiratory syndrome virus (PRRSV-NA), ...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2015.08.001

    authors： Zhang M,Xie Z,Xie L,Deng X,Xie Z,Luo S,Liu J,Pang Y,Khan MI

    更新日期：2015-11-01 00:00:00