当前位置： SCI文献检索 > JOURNAL OF VIROLOGICAL METHODS期刊下所有文献 > Preservation of RNA and destruction of infectivity in microdissected brain tissues of Lewis rats infected with the Borna disease virus.

Preservation of RNA and destruction of infectivity in microdissected brain tissues of Lewis rats infected with the Borna disease virus.

Abstract：

:Laser microdissection combined with real-time RT-PCR presents an advanced tool to quantify particular RNA species in defined tissue areas. Dealing with infectious tissue samples increases the need to overcome the risk of infectivity and contamination during laser microdissection. Here, an useful method to control infectivity of frozen brain sections infected with the Borna disease virus (BDV), an enveloped RNA virus, is described. Various pre-treatments were applied prior to laser microdissection and subsequent real-time RT-PCR. Brain sections were incubated with Vennotrade mark Vet 1 super 1% or 70% ethanol for 30, 60 and 90min, followed by quantification of infectious virus and RNA recovery using laser microdissection. Total RNA specific for the BDV nucleoprotein (BDV-N) and the cellular genes glyceraldehyde-3-phosphate dehydrogenase (GAPDH), succinate-ubiquinone reductase (SDHA) and hypoxanthine phosphoribosyl-transferase-1 (HPRT) was measured by real-time RT-PCR and compared to BDV-infected control samples. After 30 min incubation with both disinfectants, no infectious virus was isolated, while sufficient cDNA copy numbers were amplified. As tissue morphology was best preserved after ethanol treatment, 30min incubation with 70% ethanol was selected as the method of choice to prevent infectivity of BDV. This procedure represents a suitable pre-treatment option to ensure adequate safety of virus infected central nervous system tissue.

journal_name

J Virol Methods

journal_title

Journal of virological methods

authors

Porombka D,Herzog S,Baumgärtner W,Herden C

doi

10.1016/j.jviromet.2006.03.024

subject

Has Abstract

pub_date

2006-08-01 00:00:00

pages

247-53

issue

2

eissn

0166-0934

issn

1879-0984

pii

S0166-0934(06)00110-8

journal_volume

135

pub_type

杂志文章

相关文献

JOURNAL OF VIROLOGICAL METHODS文献大全
  • Structural and biological properties of Cucumber mosaic virus particles carrying hepatitis C virus-derived epitopes.

    abstract：:The Cucumber mosaic virus (CMV) is a three-component isodiametric plant virus with an extremely wide host range, present worldwide. A pseudorecombinant form has been described, deriving from the RNA3 component of the CMV-S strain, carrying the coat protein (CP) gene, and the RNA 1, 2 components of the CMV-D strain. Th...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2008.10.005

    authors： Nuzzaci M,Bochicchio I,De Stradis A,Vitti A,Natilla A,Piazzolla P,Tamburro AM

    更新日期：2009-02-01 00:00:00

  • Use of enzyme amplification in an ELISA to increase sensitivity of detection of barley yellow dwarf virus in oats and in individual vector aphids.

    abstract：:A new technique of alkaline phosphatase amplification in an ELISA (amplified ELISA) was used to increase the sensitivity of detection of barley yellow dwarf virus (BYDV) from oat plant sap and in individual vector aphids. Amplified ELISA differs from conventional direct double antibody sandwich ELISA (DAS-ELISA) in th...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(87)90056-5

    authors： Torrance L

    更新日期：1987-02-01 00:00:00

  • A diagnostic method based on MGB probes for rapid detection and simultaneous differentiation between virulent and vaccine strains of avian paramyxovirus type 1.

    abstract：:Newcastle disease virus (NDV), also designated avian paramyxovirus type 1 (APMV-1), is a serious pathogen of poultry, causing highly contagious Newcastle disease (ND), with high morbidity or mortality, depending on the strain. Accordingly, rapid and reliable detection of APMV-1 and differentiation between vaccine and ...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2010.02.012

    authors： Fratnik Steyer A,Rojs OZ,Krapez U,Slavec B,Barlic-Maganja D

    更新日期：2010-06-01 00:00:00

  • Madin-Darby bovine kidney (MDBK) cells are a suitable cell line for the propagation and study of the bovine poxvirus lumpy skin disease virus.

    abstract：:Lumpy skin disease virus (LSDV) is a poxvirus that causes systemic disease in cattle, resulting in substantial economic loss to affected communities. LSDV is a rapidly emerging pathogen of growing global concern that recently spread from Africa and the Middle East into Europe and Asia, impacting the cattle population ...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2020.113943

    authors： Fay PC,Cook CG,Wijesiriwardana N,Tore G,Comtet L,Carpentier A,Shih B,Freimanis G,Haga IR,Beard PM

    更新日期：2020-11-01 00:00:00

  • Novel one-step real-time RT-PCR assay for rapid and specific diagnosis of Crimean-Congo hemorrhagic fever encountered in the Balkans.

    abstract：:Early and accurate diagnosis of Crimean-Congo hemorrhagic fever (CCHF) is essential for the treatment and outcome of the disease and prevention of its further transmission. Molecular-based diagnostic assays now serve as the front-line tool in the diagnosis of CCHF. However, the development of real-time RT-PCR assay fo...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2005.11.006

    authors： Duh D,Saksida A,Petrovec M,Dedushaj I,Avsic-Zupanc T

    更新日期：2006-05-01 00:00:00

  • Evaluation of an affordable HIV-1 virological failure assay and antiretroviral drug resistance genotyping protocol.

    abstract：:HIV-1 RNA viral load is the preferred tool to monitor virological failure during antiretroviral therapy (ART) exposure. Timely detection of virological failure can reduce the prevalence and complexity of HIV-1 drug resistance. This field evaluation further characterizes a two-step approach to identify virological fail...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2013.08.015

    authors： Bronze M,Aitken SC,Wallis CL,Steegen K,Stuyver LJ,de Wit TF,Stevens W

    更新日期：2013-12-01 00:00:00

  • A quantitative assay for measuring human foamy virus using an established indicator cell line.

    abstract：:In order to improve the accuracy for detecting human foamy virus (HFV), an indicator cell line was established by co-transfecting baby hamster kidney-21 cells with two plasmids: one containing a G418 antibiotic resistance marker and the other including the luc gene which was placed downstream of the inducible HFV long...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/s0166-0934(01)00285-3

    authors： Tai HY,Sun KH,Kung SH,Liu WT

    更新日期：2001-05-01 00:00:00

  • HCV core antigen as an alternate test to HCV RNA for assessment of virologic responses to all-oral, interferon-free treatment in HCV genotype 1 infected patients.

    abstract：:In light of the advances in HCV therapy, simplification of diagnosis confirmation, pre- treatment diagnostic workup and treatment monitoring is required to ensure broad access to interferon-free therapies. HCV core antigen (HCV cAg) testing is rapid, giving results in approximately 60min, and less expensive than HCV R...

    journal_title：Journal of virological methods

    pub_type： 临床试验,杂志文章

    doi：10.1016/j.jviromet.2017.03.002

    authors： Rockstroh JK,Feld JJ,Chevaliez S,Cheng K,Wedemeyer H,Sarrazin C,Maasoumy B,Herman C,Hackett J Jr,Cohen DE,Dawson GJ,Cloherty G,Pawlotsky JM

    更新日期：2017-07-01 00:00:00

  • Use of the immuno-gold technique for in situ localization of cauliflower mosaic virus (CaMV) particles and the major protein of the inclusion bodies.

    abstract：:We show that the immuno-gold technique adapted to electron microscopy is a sensitive method for in situ localization of viral proteins in plant cells. Using antisera raised against cauliflower mosaic virus and the viroplasm major protein (VmP) we obtained a selective labelling of the viral particles and of the viropla...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(84)90053-3

    authors： Giband M,Stoeckel ME,Lebeurier G

    更新日期：1984-12-01 00:00:00

  • Characterization of the mixture of genotypes of a Citrus tristeza virus isolate by reverse transcription-quantitative real-time PCR.

    abstract：:A multiplex real-time PCR assay was developed to detect and quantify the Citrus tristeza virus (CTV) genotypic mixture present in infected plants. CTV isolate FS627, a complex Florida isolate containing T36, T30 and VT genotypes and its aphid transmitted subisolates was used. The relative quantitative assay was carrie...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2009.12.001

    authors： Ananthakrishnan G,Venkataprasanna T,Roy A,Brlansky RH

    更新日期：2010-03-01 00:00:00

  • Development of a genome copy specific RT-qPCR assay for divergent strains of type 2 porcine reproductive and respiratory syndrome virus.

    abstract：:Porcine reproductive and respiratory syndrome virus (PRRSV) became a significant pathogen of swine upon its emergence in the late 1980s and since then has exemplified a rapidly evolving, constantly re-emerging pathogen. In addition to the challenges faced in development of vaccines and diagnostics, research on the bas...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2015.02.007

    authors： Spear A,Faaberg KS

    更新日期：2015-06-15 00:00:00

  • Comparison of detection and quantification of HBV DNA in chronic HBeAg negative and positive patients by Abbott RealTime HBV and Roche Cobas TaqMan HBV assays.

    abstract：:Monitoring the serum hepatitis B viral DNA levels with sensitive realtime PCR assays is strongly recommended for the management of patients with chronic HBV infection. This study compares the performance of two realtime HBV quantitative PCR assays with samples from chronic HBeAg(+) and HBeAg(-) patients. ...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2013.06.036

    authors： Morris CJ,Hill M,de Medina M,Herman C,Cloherty GA,Martin P

    更新日期：2013-11-01 00:00:00

  • An alternative method for the synthesis of competitor RNA transcripts useful for specific detection and quantitation of dengue virus serotype 2 genome and replicative intermediate RNA.

    abstract：:The development of a quantitative-competitive reverse transcription-PCR (RT-PCR) assay to quantify dengue virus (DEN) genome (vRNA) and its replicative intermediate RNA (vRI) is described. A highly conserved region located on the DEN capsid-premembrane genes was used to produce a competitor RNA molecule which contains...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2008.05.007

    authors： Vaughan G,Gonzalez-Hernandez Y,Gudino JC,Olivera H,Landa-Piedra A,Escobar-Gutierrez A

    更新日期：2008-09-01 00:00:00

  • Molecular characterization of a cDNA clone encoding the Epstein-Barr virus (EBV) DNase.

    abstract：:RNA from IdUrd-treated P3HR1 cells was used for the construction of a cDNA library and screened with B95-8 EBV DNA BamHI fragment B and G probes. One clone, BG9, containing a 1.7 kb cDNA insert was further studied. Complete DNA sequence analysis revealed that BG9 encompassed the B95-8 EBV DNA sequences from nucleotide...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(90)90107-q

    authors： Chen MR,Hsu TY,Chen JY,Yang CS

    更新日期：1990-08-01 00:00:00

  • Generation and characterization of an H5N1 avian influenza virus hemagglutinin glycoprotein pseudotyped lentivirus.

    abstract：:An H5N1 avian influenza virus (AIV) hemagglutinin (HA) protein pseudotyped lentivirus, HIV/H5-HA, was generated, characterized in vitro and evaluated for its ability to induce protective immunity against virulent wild type AIV in mice. The HIV/H5-HA virus was able to infect 293T, BHK, Vero, PK-15, MDCK cells but not I...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2008.08.016

    authors： Zhang S,Xiao L,Zhou H,Yu Z,Chen H,Guo A,Jin M

    更新日期：2008-12-01 00:00:00

  • Use of non-radioactively labeled DNA probes for the detection of a baculovirus from Penaeus monodon by in situ hybridization on fixed tissue.

    abstract：:Clones isolated from a genomic library of the baculovirus PmSNPV (= MBV) were used to prepare DNA probes for detection of PmSNPV in shrimp tissue. Davidson's AFA fixed shrimp tissues were probed using DNA that had been labeled with the hapten, digoxigenin, by an in situ hybridization assay. The DNA probes readily dist...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(94)90043-4

    authors： Poulos BT,Mari J,Bonami JR,Redman R,Lightner DV

    更新日期：1994-09-01 00:00:00

  • Comparison of PCR primer pairs in the detection of human rhinoviruses in nasopharyngeal aspirates.

    abstract：:The development of a rapid and highly sensitive PCR assay for the detection of human rhinoviruses (HRVs) in nasopharyngeal aspirates is described. Two simple and fast commercial RNA extraction methods and four primer pairs were compared. The most sensitive RNA extraction method (Ultraspec) and primer pair (A) were app...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/s0166-0934(97)00049-9

    authors： Santti J,Hyypiä T,Halonen P

    更新日期：1997-06-01 00:00:00

  • Simple and specific method for detection of antibodies against hepatitis E virus in mammalian species.

    abstract：:Hepatitis E virus (HEV) is the causative agent of hepatitis E, a food- and water-borne disease. In developed countries, consumption of meats from pigs, wild boars and deer is a major source of infection. Although HEV and HEV-related viruses have been detected in many animal species, their zoonotic potential and preval...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2016.07.030

    authors： Yonemitsu K,Terada Y,Kuwata R,Nguyen D,Shiranaga N,Tono S,Matsukane T,Yokoyama M,Suzuki K,Shimoda H,Takano A,Muto M,Maeda K

    更新日期：2016-12-01 00:00:00

  • Cellufine sulfate column chromatography as a simple, rapid, and effective method to purify dengue virus.

    abstract：:Conventional method to purify/concentrate dengue virus (DENV) is time-consuming with low virus recovery yield. Herein, we applied cellufine sulfate column chromatography to purify/concentrate DENV based on the mimicry between heparan sulfate and DENV envelope protein. Comparative analysis demonstrated that this new me...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2016.04.023

    authors： Kanlaya R,Thongboonkerd V

    更新日期：2016-08-01 00:00:00

  • HPV antibody detection by ELISA with capsid protein L1 fused to glutathione S-transferase.

    abstract：:An alternative enzyme linked immunosorbent assay (ELISA) system was developed to analyze antibodies to human papillomavirus capsid antigens. The assay uses glutathione crosslinked to casein to capture the major capsid protein L1 from human papillomavirus (HPV) types 6b, 16 and 18 fused to glutathione S-transferase (GS...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/s0166-0934(02)00134-9

    authors： Sehr P,Müller M,Höpfl R,Widschwendter A,Pawlita M

    更新日期：2002-10-01 00:00:00

  • A single nucleotide polymorphism-based technique for specific characterization of YO and YN isolates of Potato virus Y (PVY).

    abstract：:One of the most important properties used to classify Potato virus Y (PVY) isolates is their ability to induce (PVY(N)) or not (PVY(O)) veinal necrosis symptoms on the indicator host plant Nicotiana tabacum cv. Xanthi. As an alternative to biological assays, several serological and molecular detection tools have been ...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2005.01.003

    authors： Jacquot E,Tribodet M,Croizat F,Balme-Sinibaldi V,Kerlan C

    更新日期：2005-04-01 00:00:00

  • Accuracy of rotavirus diagnosis: modified genome electrophoresis versus electron microscopy.

    abstract：:530 human faecal samples were examined for the presence of rotavirus by electron microscopy (EM) and by a modified electrophoretic analysis of viral genome (PAGE). 516 stools gave identical results by both methods (97.4% agreement). The proportion of EM+, PAGE- samples (1/530) was significantly lower than the proporti...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(88)90038-9

    authors： Pyndiah N,Béguin R,Richard J,Charles M,Rey A,Bonifas V

    更新日期：1988-05-01 00:00:00

  • Interferon and antibody titrations using haemagglutinating Togaviridae and trypsinized human erythrocytes.

    abstract：:Several Togaviridae of the alphavirus and flavivirus genera agglutinate trypsinized human group O erythrocytes (THOE) (Shortridge and Hu, 1976). Haemagglutinin titers of Semliki Forest virus (SFV) and Japanese encephalitis virus (JEV) measured with THOE were equivalent to, if not higher than, those obtained with Embde...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(83)90073-3

    authors： Sedmak JJ,Dixon M,Schoenherr C,Sabran JL,Grossberg SE

    更新日期：1983-02-01 00:00:00

  • The use of polylysine during negative staining of viral suspensions.

    abstract：:The use of 0.1% aqueous solution of polylysine (poly-L-lysine) is proposed as a prior step to negative staining of viral or particle suspensions. Particles spread better on films precoated with polylysine than with other substances used for the same purpose. This applies particularly to samples from sucrose or CsCl gr...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(85)90121-1

    authors： Barth OM

    更新日期：1985-05-01 00:00:00

  • Rapid detection of avian influenza virus H5N1 in chicken tracheal samples using an impedance aptasensor with gold nanoparticles for signal amplification.

    abstract：:Highly pathogenic avian influenza virus H5N1 is a continuous threat to public health and poultry industry. The recurrence of the H5N1 led us to develop a robust, specific, and rapid detection method for the virus. In this study, an impedance aptasensor was developed for the virus detection using specific H5N1 aptamer ...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2016.07.018

    authors： Karash S,Wang R,Kelso L,Lu H,Huang TJ,Li Y

    更新日期：2016-10-01 00:00:00

  • Cost-effective HIV-1 virological monitoring in resource-limited settings using a modified commercially available qPCR RNA assay.

    abstract：:Virological monitoring through plasma viral load (PVL) quantification is essential for clinical management of HIV patients undergoing antiretroviral treatment (ART), and for detecting treatment failure. Quantitative PCR (qPCR)-based tests are the gold standard for measuring PVL. Largely because of their high cost, how...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2017.05.007

    authors： Boobalan J,Torti A,Dinesha TR,Solomon SS,Balakrishnan P,Saravanan S

    更新日期：2017-10-01 00:00:00

  • Simple microwave and thermal cycler boiling methods for preparation of cervicovaginal lavage cell samples prior to PCR for human papillomavirus detection.

    abstract：:Sample preparation is an important step in the detection of viral DNA by the polymerase chain reaction (PCR) technique. The method used should achieve release of cellular DNA with the minimum of manipulation steps so as to reduce the possibility of contamination. The present report demonstrates that either microwaving...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(93)90009-g

    authors： Lou YK,Qin H,Molodysky E,Morris BJ

    更新日期：1993-09-01 00:00:00

  • Evaluation of a prototype HCV NS5b assay for typing strains of hepatitis C virus isolated from Tunisian haemodialysis patients.

    abstract：:Hepatitis C virus (HCV) strains isolated from 68 haemodialysis Tunisian patients exhibiting chronic infection were genotyped targeting the NS5b region of the HCV genome using a prototype assay developed by Bayer HealthCare-Diagnostics (TRUGENE NS5b HCV). The overall results were compared to those obtained with another...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2004.03.002

    authors： Othman SB,Trabelsi A,Monnet A,Bouzgarrou N,Grattard F,Beyou A,Bourlet T,Pozzetto B

    更新日期：2004-08-01 00:00:00

  • High-throughput real-time PCR for early detection and quantitation of arenavirus Tacaribe.

    abstract：:Arenaviruses merit significant attention both as causative agents of endemic hemorrhagic fevers and as model systems to study the immune response to acute and persistent viral infections. Development of highly sensitive quantitative screening methods to detect arenavirus is critical for early diagnosis of patients, to...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2009.04.001

    authors： Grajkowska LT,Pedras-Vasconcelos JA,Sauder C,Verthelyi D,Puig M

    更新日期：2009-08-01 00:00:00

  • Isolation of mutants of Aujeszky's disease virus with antigenically altered glycoprotein E by affinity chromatography using monoclonal antibodies.

    abstract：:An efficient method for isolation of virus mutants with antigenically altered proteins is described. The method is based on the separation of viruses with wild-type and antigenically altered proteins by affinity chromatography using monoclonal antibodies (MAbs). A nonessential glycoprotein E (gE) of Aujeszky's disease...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/s0166-0934(98)00137-2

    authors： Morenkov OS,Smirnov SV,Vrublevskaya VV

    更新日期：1999-01-01 00:00:00