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Cellufine sulfate column chromatography as a simple, rapid, and effective method to purify dengue virus.

Abstract：

:Conventional method to purify/concentrate dengue virus (DENV) is time-consuming with low virus recovery yield. Herein, we applied cellufine sulfate column chromatography to purify/concentrate DENV based on the mimicry between heparan sulfate and DENV envelope protein. Comparative analysis demonstrated that this new method offered higher purity (as determined by less contamination of bovine serum albumin) and recovery yield (as determined by greater infectivity). Moreover, overall duration used for cellufine sulfate column chromatography to purify/concentrate DENV was approximately 1/20 of that of conventional method. Therefore, cellufine sulfate column chromatography serves as a simple, rapid, and effective alternative method for DENV purification/concentration.

journal_name

J Virol Methods

journal_title

Journal of virological methods

authors

Kanlaya R,Thongboonkerd V

doi

10.1016/j.jviromet.2016.04.023

subject

Has Abstract

pub_date

2016-08-01 00:00:00

pages

174-7

eissn

0166-0934

issn

1879-0984

pii

S0166-0934(16)30108-2

journal_volume

234

pub_type

杂志文章

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