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Ferrihydrite treatment to mitigate inhibition of RT-qPCR virus detection from large-volume environmental water samples.

Abstract：

:Molecular assays are currently the most widely used method to quantify pathogenic viruses in water; however, their performance is often disrupted by matrices present in environmental samples. The present study used ferrihydrite (Fh) treatment to mitigate inhibition of RT-qPCR virus detection from environmental water concentrates. Fh treatment was performed to improve the detection of spiked Aichi virus 1 (AiV) and Qβ bacteriophage (Qβ) in commercial humic acids and seawater concentrates. The optimal Fh doses were found to be 200, 500, and 1000 mgFe/L for humic acid concentrates at UV254 of 1.5, 3.0, and 6.0 cm-1 respectively, whereas this value was 1000 mgFe/L for the seawater concentrates at UV254 of 1.5 cm-1. At these optimal doses, the recoveries of spiked AiV and Qβ ranged from 2% to 12% in the humic acid and seawater concentrates, respectively. In addition, high levels of indigenous viruses (including AiV ≥ 2.81 log10 copies/mL and pepper mild mottle virus ≥ 2.77 log10 copies/mL) were detected from seawater concentrates after Fh treatment, while none were detected without Fh treatment. Fh treatment effectively mitigated the inhibitive effects from environmental water samples.

journal_name

J Virol Methods

journal_title

Journal of virological methods

authors

Canh VD,Osawa H,Inoue K,Kasuga I,Takizawa S,Furumai H,Katayama H

doi

10.1016/j.jviromet.2018.10.018

subject

Has Abstract

pub_date

2019-01-01 00:00:00

pages

60-67

eissn

0166-0934

issn

1879-0984

pii

S0166-0934(18)30407-5

journal_volume

263

pub_type

杂志文章

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