Abstract：
:The development of an effective prophylactic vaccine is still necessary to improve the safety of the conventional although-discontinued smallpox vaccine, and to protect from the threat of deliberate release of variola virus. This need also arises from the number of new cases of animal orthopoxvirus infections each year, and to reduce the risk to animal handlers. Fowlpox (FP) recombinants only replicate in avian species and have been developed against human infectious diseases, as they can elicit an effective immune response, are not cross-reactive immunologically with vaccinia, and represent safer and more promising immunogens for immunocompromised individuals. The aim of this study was the characterisation of two new fowlpox recombinants expressing the A33R vaccinia virus gene either alone (FP(A33R)) or with the green fluorescent protein (FP(A33R-GFP)) to verify whether GFP can affect the expression of the transgene. The results show that both FP(A33R) and FP(A33R-GFP) can express A33R correctly, but A33R mRNA and protein synthesis are higher by FP(A33R) than by FP(A33R-GFP). Therefore, GFP co-expression does not prevent, but can reduce the level of a vaccine protein, and may affect the protective efficacy of the immune response.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Bissa M,Pacchioni SM,Zanotto C,De Giuli Morghen C,Radaelli Adoi
10.1016/j.jviromet.2012.09.009subject
Has Abstractpub_date
2013-01-01 00:00:00pages
172-6issue
1eissn
0166-0934issn
1879-0984pii
S0166-0934(12)00320-5journal_volume
187pub_type
杂志文章abstract：:A one-step centrifugal ultrafiltration method was developed to enhance rapid detection of human enteric viruses and co-occurring viruses in wastewater. Samples were collected pre- and post-UV treatment at two full-scale tertiary municipal wastewater treatment plants in Calgary, Canada. Viruses were concentrated from 1...
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pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2018-05-01 00:00:00
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pub_type： 杂志文章
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更新日期：1996-04-05 00:00:00
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pub_type： 杂志文章
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更新日期：2003-02-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/0166-0934(88)90115-2
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abstract：:Rapid detection of CMV-DNA in urine specimens by dot-blot hybridization was compared to conventional virus isolation and to virus identification using solid-phase immunoelectron microscopy (SPIEM). To detect viral DNA, 32P-labeled EcoR1 J fragment of CMV-DNA was used as a probe in the hybridization assay. In addition,...
journal_title：Journal of virological methods
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