Abstract：
:A one-step centrifugal ultrafiltration method was developed to enhance rapid detection of human enteric viruses and co-occurring viruses in wastewater. Samples were collected pre- and post-UV treatment at two full-scale tertiary municipal wastewater treatment plants in Calgary, Canada. Viruses were concentrated from 100mL wastewater samples through direct centrifugation using the Centricon Plus-70 ultrafilter. Seven viruses, including norovirus, rotavirus, sapovirus, astrovirus, enterovirus, adenovirus and JC virus, were tested using real-time quantitative PCR (rt-qPCR) and cell culture. All of the viruses were detected in pre- and post-UV samples by rt-qPCR, with rotavirus the most numerous (6.6 log10 GE copies/L). Infectious viruses, by cell culture, were found in all tested pre-UV samples but only in one post-UV sample. The results were comparable and consistent to that obtained using virus adsorption-elution method, indicating that the centrifugal ultrafiltration method is adequate to retain the viruses and maintain their infectivity during processing. As a simple, rapid and cost-effective method to screen wastewater viruses, this one-step centrifugal ultrafiltration method may serve as an effective approach to assess virus removal and gain knowledge of human virus activity during wastewater treatment.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Qiu Y,Lee BE,Ruecker NJ,Neumann N,Ashbolt N,Pang Xdoi
10.1016/j.jviromet.2016.09.010subject
Has Abstractpub_date
2016-11-01 00:00:00pages
150-153eissn
0166-0934issn
1879-0984pii
S0166-0934(16)30435-9journal_volume
237pub_type
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