Abstract:
:A novel and rapid EBNA-1 titration method has been developed which uses immunoprecipitation of specific DNA-protein complexes with EBNA-1-positive serum. The method is more sensitive than the conventional immunofluorescence method and has potential value as a diagnostic reagent for clinical laboratories. TPA induction of putative anti-EBNA-1 protein of cellular origin is discussed, which may play a key role for the shift from latent to lytic replication of EBV.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Nonoyama M,Wen LT,Tabata T,Tanaka Adoi
10.1016/0166-0934(88)90062-6subject
Has Abstractpub_date
1988-09-01 00:00:00pages
161-70issue
1-4eissn
0166-0934issn
1879-0984pii
0166-0934(88)90062-6journal_volume
21pub_type
杂志文章abstract::This study assesses the ability of quantitative real-time PCR to measure the effects of virus DNA polymerase inhibitors on EBV DNA and late mRNAs syntheses in EBV-producing cell lines. In-house real-time quantitative PCRs were used to measure EBV DNA (thymidine kinase) and mRNAs (BLLF1 gene/gp350/220, BVRF2 gene/prote...
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journal_title:Journal of virological methods
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更新日期:2013-12-01 00:00:00
abstract::Sabin type 3 polio vaccine virus is the most common cause of poliovaccine associated paralytic poliomyelitis. Vaccine associated paralytic poliomyelitis cases have been associated with Sabin type 3 revertants containing a single U to C substitution at bp 472 of Sabin type 3. A rapid method of identification of Sabin t...
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journal_title:Journal of virological methods
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更新日期:2014-09-01 00:00:00
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journal_title:Journal of virological methods
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abstract::Congenital human cytomegalovirus infections are the major infectious cause of birth defects in the United States. How this virus crosses the placenta and causes fetal disease is poorly understood. Guinea pig cytomegalovirus (GPCMV) is a related virus that provides an important model for studying cytomegaloviral congen...
journal_title:Journal of virological methods
pub_type: 杂志文章
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更新日期:2008-05-01 00:00:00
abstract::Feline infectious peritonitis (FIP) is a fatal disease in cats worldwide. The aim of this study was to test two commercially available reaction mixtures in a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay to detect feline Coronavirus (FCoV) in body cavity effusions of cats with and withou...
journal_title:Journal of virological methods
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journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/j.jviromet.2018.05.009
更新日期:2018-10-01 00:00:00
abstract::A technique for coupling foot-and-mouth disease virus (FMDV) to tanned sheep red blood cells (SRBC) is reported. Different parameters influencing the procedure were studied. Subtypes C2, C3, O1 and A24 were used as antigens, and guinea pig hyperimmune sera obtained were tested for specific antibody in passive hemagglu...
journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/0166-0934(88)90131-0
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journal_title:Journal of virological methods
pub_type: 杂志文章
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更新日期:2014-12-01 00:00:00
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journal_title:Journal of virological methods
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更新日期:2004-03-15 00:00:00
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/j.jviromet.2004.09.019
更新日期:2005-02-01 00:00:00
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
pub_type: 杂志文章
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更新日期:2011-10-01 00:00:00
abstract::We describe a laboratory information system employing a virus and chlamydia database developed over seven years which is used by a regional laboratory, performing virus and chlamydia testing. The information system is implemented on a University mainframe computer and accessed by computer terminals in the laboratory. ...
journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
pub_type: 杂志文章
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abstract::The latent membrane protein 1 (LMP1) of Epstein-Barr virus (EBV) is one of two postulated viral oncogenic proteins. Sequence variations, and in particular a 30 base pair deletion variant called CAO, may define different disease populations. We developed a panel of rat monoclonal antibodies (MAb) specific for the non-w...
journal_title:Journal of virological methods
pub_type: 杂志文章
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更新日期:2004-03-01 00:00:00
abstract::Human papillomavirus (HPV) infection is the primary cause of cervical cancer. The Quantivirus(®) HPV E6/E7 RNA 3.0 assay (DiaCarta, CA, USA) detects E6/E7 mRNA of 13 high risk subtypes and 6 low risk subtypes. Cervical specimens collected in PreservCyt were processed for HPV detection. Cervical biopsies were taken onl...
journal_title:Journal of virological methods
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