Abstract:
:The bacteriophage phiDNA polymerase amplifies circular DNA in a rolling circle amplification mechanism. This characteristic was applied to amplify and clone the complete circular DNA genome of a begomovirus. Total DNA extracted from infected tissue was used as the template of an amplification reaction using the commercial kit TempliPhi (Amersham Biosciences). The amplified DNA could be used for direct sequencing and was cloned after digestion with a single cutting restriction endonuclease. The use of this enzyme simplified the cloning steps and increased the cloning efficiency of the complete genome of a circular plant DNA virus.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Inoue-Nagata AK,Albuquerque LC,Rocha WB,Nagata Tdoi
10.1016/j.jviromet.2003.11.015subject
Has Abstractpub_date
2004-03-15 00:00:00pages
209-11issue
2eissn
0166-0934issn
1879-0984pii
S0166093403003938journal_volume
116pub_type
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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