Abstract：
:HSV-1-based vectors have been widely used to achieve targeted delivery of genes into the nervous system. In the current study, we aim to use shRNA-containing HSV-1-based gene delivery system for the therapy of HSV-2 infection. Guinea pigs were infected intravaginally with HSV-2 and scored daily for 100 days for the severity of vaginal disease. HSV-2 shRNA-containing HSV-1 was applied intravaginally daily between 8 and 14 days after HSV-2 challenge. Delivery of HSV-2 shRNA-containing HSV-1 had no effect on the onset of disease and acute virus shedding in animals, but resulted in a significant reduction in both the cumulative recurrent lesion days and the number of days with recurrent disease. Around half of the animals in the HSV-2 shRNA group did not develop recurrent disease 100 days post HSV-2 infection. In conclusion, HSV-2 shRNA-containing HSV-1 particles are effective in reducing the recurrence of genital herpes caused by HSV-2.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Liu Z,Xiang Y,Wei Z,Yu B,Shao Y,Zhang J,Yang H,Li M,Guan M,Wan J,Zhang Wdoi
10.1016/j.jviromet.2013.06.037subject
Has Abstractpub_date
2013-11-01 00:00:00pages
353-8issue
2eissn
0166-0934issn
1879-0984pii
S0166-0934(13)00251-6journal_volume
193pub_type
杂志文章abstract：:A cell line which can be used in a simple, sensitive, and rapid histochemical assay was isolated for detection of herpes simplex virus (HSV). The cell line was derived by selection of G418 resistant colonies following co-transfection of baby hamster kidney cells with a plasmid which contains a G418 antibiotic resistan...
journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/0166-0934(92)90110-y
更新日期：1992-08-01 00:00:00
abstract：:The genes encoding the Erns and E2 antigen epitopes of classical swine fever virus (CSFV) were expressed as a chimeric protein in Escherichia coli BL21 by pET expression system. The antigenicity of the expressed protein CnC2 was identified by indirect enzyme-linked immunoabsorbant assay (ELISA) and immunoblot with ant...
journal_title：Journal of virological methods
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doi：10.1016/j.jviromet.2011.12.006
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abstract：:A TaqMan real-time RT-PCR was developed to detect and quantify RNA-targets from the non-circulative, non-persistently transmitted Plum pox virus (PPV) in individual fresh or aphids captured previously and squashed on paper. Reliable quantitation ranged from 40 up to 4 x 10(8) copies of control transcripts. This techni...
journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2005.05.011
更新日期：2005-09-01 00:00:00
abstract：:A 2-plasmid/4-cosmid-based system of mutagenesis is described for construction of herpes simplex virus type 1 (HSV-1) variants with point mutations in the protease gene. The system was used to reconstruct a mutant virus (V701) with Tyr30 to Phe and Ala48 to Val mutations in HSV-1 protease that exhibits the temperature...
journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/0166-0934(95)01984-7
更新日期：1996-04-05 00:00:00
abstract：:Integration of reverse transcribed viral DNA of HIV into host chromosomes is mediated by the viral enzyme, integrase. This enzymatic activity can be monitored in vitro by integration of a small labeled DNA (donor) into a second unlabeled DNA (target). The methodology usually involves isotope labeling and gel electroph...
journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/0166-0934(96)02033-2
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journal_title：Journal of virological methods
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abstract：:Endothelial cell tropism is an inherent property of isolates of human cytomegalovirus (HCMV), and has been proposed as a pathogenicity factor of HCMV. Mutational approaches can be applied for analysis of the molecular determinants of endothelial cell tropism, but the evaluation of mutants is limited by the low reliabi...
journal_title：Journal of virological methods
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doi：10.1016/j.jviromet.2010.03.031
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abstract：:Tomato ringspot virus (ToRSV), Tobacco ringspot virus (TRSV) and Tobacco rattle virus (TRV) are transmitted to healthy plants by viruliferous nematodes in the soil. A method was developed for extraction of genomic viral RNA from virus particles carried within nematodes and a sensitive nested RT-PCR detection assay. Th...
journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2008.10.002
更新日期：2009-01-01 00:00:00
abstract：:Receptor-mediated endocytosis was employed for a highly efficient transport of oligodeoxynucleotides into hepatoma cell line PLC/PRF/5. The oligodeoxynucleotides were bound to the asialofetuin-poly-L-lysine conjugate and this complex was internalized by the cells via asialoglycoprotein receptor, an endocytic receptor ...
journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/0166-0934(93)90181-p
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更新日期：2010-05-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
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abstract：:Nucleic-acid amplification technology (NAT) assays have been implemented for HCV and HIV-1 in the United States, and many parts of Europe, Australia and Asia. Nucleic acid detection assays utilize many different technologies, and the WHO International Standards for nucleic acid tests are widely used to compare them. C...
journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2004.01.021
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/0166-0934(85)90038-2
更新日期：1985-06-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/0166-0934(93)90091-5
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journal_title：Journal of virological methods
pub_type： 杂志文章
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更新日期：1995-10-01 00:00:00
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/s0166-0934(01)00319-6
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journal_title：Journal of virological methods
pub_type： 杂志文章
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journal_title：Journal of virological methods
pub_type： 杂志文章
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journal_title：Journal of virological methods
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更新日期：1990-02-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
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更新日期：2016-03-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
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journal_title：Journal of virological methods
pub_type： 杂志文章
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更新日期：2018-08-01 00:00:00
abstract：:A pandemic caused by a novel influenza A virus (H1N1) poses a serious public health threat. In this study, a real-time reverse transcriptase PCR (RT-PCR) assay based on the hemagglutinin gene was developed that discriminates the novel H1N1 from swine influenza virus, seasonal H1N1/H3N2 virus and the highly pathogenic ...
journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2009.09.021
更新日期：2010-02-01 00:00:00
abstract：:A real-time reverse transcription-polymerase chain reaction (qRT-PCR) assay was developed for the fast and highly sensitive detection of the sacbrood virus (SBV) genome and applied to honeybee samples. Using plasmid DNA containing a partial SBV genome and diluted serially, as few as 1×10(2)copies/μl (correlation co-ef...
journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2011.10.014
更新日期：2012-01-01 00:00:00
abstract：:A simple sequencing-based assay is described for genotyping of hepatitis C virus (HCV). RT-PCR was employed to amplify a 237-nucleotide-long fragment from the 5' untranslated region (UTR) of the genome using one biotinylated and one normal primer. Subsequent to capture of the PCR products on streptavidin-coated beads,...
journal_title：Journal of virological methods
pub_type： 杂志文章
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更新日期：2003-05-01 00:00:00
abstract：:Rice tungro disease (RTD) is a recurring disease affecting rice farming especially in the South and Southeast Asia. The disease is commonly diagnosed by visual observation of the symptoms on diseased plants in paddy fields and by polymerase chain reaction (PCR). However, visual observation is unreliable and PCR can be...
journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2016.12.001
更新日期：2017-02-01 00:00:00
abstract：:Goatpox disease and cystic hydatidosis may be simultaneously endemic in a given area. Their pathogens are goatpox virus (GPV) and Echinococcus granulosus (E. granulosus), respectively. Both E. granulosus EG95 subunit vaccine and live-attenuated GPV AV41 vaccine have been widely used for prevention of both diseases in ...
journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2018.08.002
更新日期：2018-11-01 00:00:00
abstract：:A large number of Norwalk-like viruses (NLVs) have been identified from stool samples by RT-PCR by amplifying part of the polymerase-coding gene. A set of probes were selected based on sequence analysis of the viruses circulating in Finland during the years 1996-97 for confirmation of the findings by hybridization. A ...
journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/s0166-0934(99)00115-9
更新日期：1999-12-01 00:00:00
