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A new method of RNA preparation for detection of hepatitis A virus in environmental samples by the polymerase chain reaction.

Abstract：

:A new method based on RNA preparation from shellfish by polyethylene glycol (PEG) precipitation and trichloroacetic acid (TCA) extraction was developed for the detection of hepatitis A virus (HAV) by the polymerase chain reaction (PCR). The extraction provides high yield and the extracted RNA is undegraded. This method proved to be particularly useful for detection of RNA viruses from environmental samples by PCR.

journal_name

J Virol Methods

journal_title

Journal of virological methods

authors

Yang F,Xu X

doi

10.1016/0166-0934(93)90091-5

subject

Has Abstract

pub_date

1993-06-01 00:00:00

pages

77-84

issue

1

eissn

0166-0934

issn

1879-0984

pii

0166-0934(93)90091-5

journal_volume

43

pub_type

杂志文章

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