Abstract：
:Noroviruses are the leading cause of nonbacterial gastroenteritis outbreaks in the United States, some of which are caused by the ingestion of contaminated water. Detection and genotypic characterization of noroviruses is commonly performed by reverse transcription-polymerase chain reaction (RT-PCR) followed by sequencing. However, sequencing of products amplified from environmental water samples is often hindered by the co-amplification of non-specific cDNA. To overcome this issue, a generic microarray was evaluated to genotype noroviruses by probe hybridization. RT-PCR amplicons are used in a single base extension (SBE) reaction where genotype-specific probes are labeled and then hybridized to an Affymetrix GeneChip GenFlex Tag Array for detection. Using a standardized, multiplex SBE reaction, genotyping of representative strains was accomplished through the identification of genotype-specific patterns of positive hybridization results. Furthermore, the SBE-GenFlex array method was successful in the genotype identification of noroviruses seeded into tap and Ohio River water samples. This study shows the utility of using a microarray to genotype noroviruses in complex environmental matrices.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Brinkman NE,Fout GSdoi
10.1016/j.jviromet.2008.03.010subject
Has Abstractpub_date
2009-03-01 00:00:00pages
8-18issue
1-2eissn
0166-0934issn
1879-0984pii
S0166-0934(08)00089-Xjournal_volume
156pub_type
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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abstract：:Indicator cell lines are useful biological tools for monitoring virus infection. In order to monitor infection with bovine immunodeficiency virus (BIV) in vitro, an indicator cell line derived from baby hamster kidney cells which contains integrated copies of an enhanced green fluorescent protein gene driven by the BI...
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journal_title：Journal of virological methods
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更新日期：2000-07-01 00:00:00
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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更新日期：2015-06-15 00:00:00
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
pub_type： 杂志文章
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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更新日期：2013-12-01 00:00:00
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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更新日期：1999-10-01 00:00:00
