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Reactivity and amplification efficiency of the NASBA HIV-1 RNA amplification system with regard to different HIV-1 subtypes.

Abstract：

:In view of the genetic diversity of the human immunodeficiency virus Type 1, we assessed the sensitivity and quantification efficiency of the HIV-1 RNA NASBA amplification system with respect to different HIV-1 subtypes and recombinants. Twenty cell culture supernatants representing 17 HIV-1 group M and 3 group O strains were tested, and NASBA RNA loads were compared with results obtained with a RT-PCR based HIV-1 RNA quantitation method, with p24-antigen concentrations and with the infective dose. The current HIV-1 RNA NASBA seemed suitable to quantitate representatives of different HIV-1 M subtypes. Differences between NASBA and RT-PCR loads were observed for certain HIV-1 M strains. Significantly lower RT-PCR loads were measured for most gag A, gag B and gag F strains, whereas NASBA detected lower copy numbers in 1 gag H strain and 1 gag H/env G recombinant. NASBA was not able to quantify 1 HIV-1 group M recombinant. Some of these differences could be explained by the presence and position of mismatches with primers. HIV-1 group O strains were not detectable by both RNA amplification methods. A firm correlation was not observed between the measured RNA loads and either the p24-antigen concentration or the infective dose.

journal_name

J Virol Methods

journal_title

Journal of virological methods

authors

Gobbers E,Fransen K,Oosterlaken T,Janssens W,Heyndrickx L,Ivens T,Vereecken K,Schoones R,van de Wiel P,van der Groen G

doi

10.1016/s0166-0934(97)00072-4

subject

Has Abstract

pub_date

1997-07-01 00:00:00

pages

293-301

issue

2

eissn

0166-0934

issn

1879-0984

pii

S0166093497000724

journal_volume

66

pub_type

杂志文章

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