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An alternative method for the synthesis of competitor RNA transcripts useful for specific detection and quantitation of dengue virus serotype 2 genome and replicative intermediate RNA.

Abstract：

:The development of a quantitative-competitive reverse transcription-PCR (RT-PCR) assay to quantify dengue virus (DEN) genome (vRNA) and its replicative intermediate RNA (vRI) is described. A highly conserved region located on the DEN capsid-premembrane genes was used to produce a competitor RNA molecule which contains an internal deletion of 70 nucleotides. The competitor provides a suitable internal control useful to quantify viral RNA from all four dengue virus (DEN 1-4) serotypes. The detection limit of the assay was found to be 100 copies per reaction. This is a rapid, simple, sensitive, inexpensive and easy method for quantitation of DEN RNA species.

journal_name

J Virol Methods

journal_title

Journal of virological methods

authors

Vaughan G,Gonzalez-Hernandez Y,Gudino JC,Olivera H,Landa-Piedra A,Escobar-Gutierrez A

doi

10.1016/j.jviromet.2008.05.007

subject

Has Abstract

pub_date

2008-09-01 00:00:00

pages

72-6

issue

1-2

eissn

0166-0934

issn

1879-0984

pii

S0166-0934(08)00168-7

journal_volume

152

pub_type

杂志文章

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