Removal of lactate dehydrogenase-elevating virus from human-in-mouse breast tumor xenografts by cell-sorting.


:Lactate dehydrogenase-elevating virus (LDV) can infect transplantable mouse tumors or xenograft tumors in mice through LDV-contaminated mouse biological materials, such as Matrigel, or through mice infected with LDV. LDV infects specifically mouse macrophages and alters immune system and tumor phenotype. The traditional approaches to remove LDV from tumor cells, by transplanting tumors into rats or culturing tumor cells in vitro, are inefficient, labor-intensive and time-consuming. Furthermore, these approaches are not feasible for primary tumor cells that cannot survive tissue culture conditions or that may change phenotype in rats. This study reports that fluorescence-activated cell sorting (FACS) is a simple and efficient approach for purifying living primary human breast tumor cells from LDV(+) mouse stromal cells, which can be completed in a few hours. When purified from Matrigel contaminated LDV(+) tumors, sorted human breast tumor cells, as well as tumors grown from sorted cells, were shown to be LDV-free, as tested by PCR. The results demonstrate that cell sorting is effective, much faster and less likely to alter tumor cell phenotype than traditional methods for removing LDV from xenograft models. This approach may also be used to remove other rodent-specific viruses from models derived from distinct tissues or species with sortable markers, where virus does not replicate in the cells to be purified.


J Virol Methods


Liu H,Bockhorn J,Dalton R,Chang YF,Qian D,Zitzow LA,Clarke MF,Greene GL




Has Abstract


2011-05-01 00:00:00














  • Filter in situ hybridisation: an evaluation of the FISH technique for HPV detection in cervical swabs.

    abstract::The filter in situ hybridisation (FISH) method for detection of HPV in cervical swabs was evaluated against the Southern blot technique on concomitant cervical biopsies. Of 73 biopsies, HPV 16 DNA sequences were found in 26 biopsies and HPV 18 sequences in 2 biopsies. Analysis by FISH of the corresponding smears detec...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Hørding U,Sebbelov A,Daugaard S,Bock JE,Norrild B

    更新日期:1989-04-01 00:00:00

  • A nonradioactive riboprobe assay for the detection of hepatitis B virus DNA in human sera.

    abstract::Sequences of hepatitis B virus DNA were detected specifically in the sera of infected individuals by a non-radioactive riboprobe nucleic acid hybridization assay. The nucleic acids contained in serum specimens were prepared by an overnight proteinase K-/SDS-digest, phenol/chloroform-extraction and ethanol-precipitatio...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Gerritzen A,Scholt B

    更新日期:1990-12-01 00:00:00

  • A novel HIV-1 reporter virus with a membrane-bound Gaussia princeps luciferase.

    abstract::HIV-1 reporter viruses are a critical tool for investigating HIV-1 infection. By having a reporter gene incorporated into the HIV-1 genome, the expressed reporter protein acts as a specific tag, thus enabling specific detection of HIV-1 infected cells. Currently existing HIV-1 reporter viruses utilize reporters for th...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Suree N,Koizumi N,Sahakyan A,Shimizu S,An DS

    更新日期:2012-07-01 00:00:00

  • Disinfection protocols for necropsy equipment in rabies laboratories: Safety of personnel and diagnostic outcome.

    abstract::In the last decades, molecular techniques have gradually been adopted for the rapid confirmation of results obtained through gold standard methods. However, international organisations discourage their use in routine laboratory investigations for rabies post-mortem diagnosis, as they may lead to false positive results...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Aiello R,Zecchin B,Tiozzo Caenazzo S,Cattoli G,De Benedictis P

    更新日期:2016-08-01 00:00:00

  • Detection and quantitation of the new world Squash leaf curl virus by TaqMan real-time PCR.

    abstract::Squash leaf curl diseases are caused by distinct virus species that are separated into two major phylogenetic groups, western and eastern hemisphere groups. The western group includes the new world Squash leaf curl virus (SLCV) which causes major losses to cucurbit production and induces severe stunting and leaf curl ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Abrahamian PE,Abou-Jawdah Y

    更新日期:2013-07-01 00:00:00

  • High-throughput real-time PCR for early detection and quantitation of arenavirus Tacaribe.

    abstract::Arenaviruses merit significant attention both as causative agents of endemic hemorrhagic fevers and as model systems to study the immune response to acute and persistent viral infections. Development of highly sensitive quantitative screening methods to detect arenavirus is critical for early diagnosis of patients, to...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Grajkowska LT,Pedras-Vasconcelos JA,Sauder C,Verthelyi D,Puig M

    更新日期:2009-08-01 00:00:00

  • A baculovirus vector derived from immediately early gene promoter of Autographa californica nuclear polyhedrosis virus.

    abstract::A transfer vector was constructed in which the neomycin resistance (neo) gene was under the control of a copy of Autographa californica nuclear polyhedrosis virus (AcMNPV) IE1 gene promoter at the p10 locus. After cotransfection of Spodoptera frugiperda (Sf9) cells with the transfer vector and infectious AcMNPV DNA, t...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Zhu FX,Qi YP,Huang YX,Bing Q

    更新日期:1996-10-01 00:00:00

  • Novel one-step real-time RT-PCR assay for rapid and specific diagnosis of Crimean-Congo hemorrhagic fever encountered in the Balkans.

    abstract::Early and accurate diagnosis of Crimean-Congo hemorrhagic fever (CCHF) is essential for the treatment and outcome of the disease and prevention of its further transmission. Molecular-based diagnostic assays now serve as the front-line tool in the diagnosis of CCHF. However, the development of real-time RT-PCR assay fo...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Duh D,Saksida A,Petrovec M,Dedushaj I,Avsic-Zupanc T

    更新日期:2006-05-01 00:00:00

  • Rapid diagnosis of influenza A. Comparison with ELISA immunocapture and culture.

    abstract::The Directigen Flu-A is an enzyme immunoassay for detecting in 15 min the influenza A nucleoproteinic antigen directly from specimens after passive adsorption on a cellulose membrane. The test was assessed using 160 frozen (-20 degrees C) specimens collected during the 1988-1989 A/H1N1 influenza epidemic and the 1989-...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Chomel JJ,Remilleux MF,Marchand P,Aymard M

    更新日期:1992-06-01 00:00:00

  • Rapid detection and quantitation of viral hemorrhagic septicemia virus in experimentally challenged rainbow trout by real-time RT-PCR.

    abstract::A quantitative real-time RT-PCR (Q-RT-PCR) was developed to detect and determine the amount of viral hemorrhagic septicemia virus (VHSV) in organs of experimentally infected rainbow trout. Primers and TaqMan probes targeting the glycoprotein (G) and the nucleoprotein (N) genes of the virus were designed. The efficienc...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Chico V,Gomez N,Estepa A,Perez L

    更新日期:2006-03-01 00:00:00

  • Real-time RT-PCR (TaqMan) assays for the detection of viruses associated with Rugose wood complex of grapevine.

    abstract::Real-time TaqMan RT-PCR (TaqMan RT-PCR) assays were developed to detect the viruses associated with Rugose wood complex of grapevines. The viruses detected were Rupestris stem pitting-associated virus in the genus Foveavirus, Grapevine virus A, Grapevine virus B and Grapevine virus D in the genus Vitivirus. The coat p...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Osman F,Rowhani A

    更新日期:2008-12-01 00:00:00

  • An indirect ELISA for the detection of antibodies against porcine reproductive and respiratory syndrome virus using recombinant nucleocapsid protein as antigen.

    abstract::An indirect enzyme-linked immunosorbent assay termed rnPRRS ELISA using baculovirus-expressed and affinity-purified viral nucleocapsid protein (rNC) antigen was developed for detecting antibodies against porcine reproductive and respiratory syndrome virus (PRRSV) in swine sera. Sera (1395) originating from different E...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Denac H,Moser C,Tratschin JD,Hofmann MA

    更新日期:1997-05-01 00:00:00

  • Chemical inactivation of hepatitis B virus: the effect of disinfectants on virus-associated DNA polymerase activity, morphology and infectivity.

    abstract::The inactivation of hepatitis B virus (HBV) using two commercially available disinfectants was analysed. Indirect evidence of virus inactivation was obtained by examining the decrease in HBV-associated DNA polymerase and HBcAg activities after treatment with increasing concentrations of disinfectant. Inactivation was ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Howard CR,Dixon J,Young P,van Eerd P,Schellekens H

    更新日期:1983-09-01 00:00:00

  • Chemiluminescent microtiter method for detecting PCR amplified HIV-1 DNA.

    abstract::A rapid and sensitive method for detecting HIV-1 DNA sequences amplified by polymerase chain reaction (PCR) is described. The method uses solution phase hybridization for rapid and efficient annealing between digoxigenin-labeled targets and biotinylated capture probes. Hybrids containing biotin are captured onto strep...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Suzuki K,Okamoto N,Watanabe S,Kano T

    更新日期:1992-07-01 00:00:00

  • A cost-effective algorithm for the diagnosis of Hepatitis C virus infection and prediction of HCV viremia in Cameroon.

    abstract::Conventional tests for antibody to Hepatitis C virus (HCV) and HCV RNA require considerable time before results are available, remain very expensive and are not adapted to many sub-Saharan African countries where HCV is endemic. The aim of this study was to evaluate the accuracy of an algorithm consisting of two HCV r...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Njouom R,Tejiokem MC,Zanga MC,Pouillot R,Ayouba A,Pasquier C,Nerrienet E

    更新日期:2006-05-01 00:00:00

  • Development of a TaqMan assay for sensitive detection of all pestiviruses infecting cattle, including the emerging HoBi-like strains.

    abstract::A real-time RT-PCR assay based on the TaqMan technology was developed for rapid and sensitive detection of pestiviruses infecting cattle, i.e., bovine viral diarrhea virus (BVDV) 1, BVDV-2, and the emerging HoBi-like pestiviruses. The assay was linear and reproducible, being able to detect as few as 10 copies of viral...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Losurdo M,Mari V,Lucente MS,Colaianni ML,Padalino I,Cavaliere N,Buonavoglia C,Decaro N

    更新日期:2015-11-01 00:00:00

  • Sodium sulphite inhibition of potato and cherry polyphenolics in nucleic acid extraction for virus detection by RT-PCR.

    abstract::Phenolic compounds from plant tissues inhibit reverse transcription-polymerase chain reaction (RT-PCR). Multiple-step protocols using several additives to inhibit polyphenolic compounds during nucleic acid extraction are common, but time consuming and laborious. The current research highlights that the inclusion of 0....

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Singh RP,Nie X,Singh M,Coffin R,Duplessis P

    更新日期:2002-01-01 00:00:00

  • TaqMan real-time and conventional nested PCR tests specific to yellow head virus genotype 7 (YHV7) identified in giant tiger shrimp in Australia.

    abstract::In 2013, a unique seventh yellow head virus genotype (YHV7) was detected in Black Tiger shrimp (Penaeus monodon) broodstock that suffered high mortality following their capture from Joseph Bonaparte Gulf (JBG) in northern Australia. To assist with its diagnosis and assessment of its distribution, prevalence and pathog...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Cowley JA,Rao M,Mohr P,Moody NJ,Sellars MJ,Crane MS

    更新日期:2019-11-01 00:00:00

  • A novel approach to the detection of classical swine fever virus by RT-PCR with a fluorogenic probe (TaqMan).

    abstract::Detection of classical swine fever virus (CSFV) and its discrimination from other pestiviruses can be achieved by virus isolation (VI) in cell cultures, antigen detection, or molecular analysis. To simplify the latter, a 5'-nuclease assay (TaqMan) was developed for the rapid and specific detection of CSFV with the min...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: McGoldrick A,Lowings JP,Ibata G,Sands JJ,Belak S,Paton DJ

    更新日期:1998-06-01 00:00:00

  • Establishment and validation of an ELISA for the quantitation of HBsAg in recombinant hepatitis B vaccines.

    abstract::Commercial enzyme-linked immunosorbent assay (ELISA) kits for the determination of the in vitro potency of recombinant hepatitis B vaccines, which detect hepatitis B surface antigen (HBsAg), have been used frequently as an alternative for traditional in vivo potency tests. With the constant need for validation procedu...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Costa CI,Delgado IF,da Costa JA,de Carvalho RF,Mouta Sda S Jr,Vianna CO,de Moraes MT

    更新日期:2011-03-01 00:00:00

  • New design of probe and central-homo primer pairs to improve TaqMan™ PCR accuracy for HBV detection.

    abstract::Quantitative PCR (qPCR) assay using TaqMan™ probe was widely used in the detection of different nucleic acids. However, this technology has several drawbacks, including false negative results caused by primer-dimer (PD) and false positive issues due to primer-probe aggregations. Here, we designed a modified TaqMan™-Mo...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Jiang W,Yue S,He S,Chen C,Liu S,Jiang H,Tong H,Liu X,Wang J,Zhang F,Sun H,Li M,Wang C

    更新日期:2018-04-01 00:00:00

  • Rapid and sensitive detection of hepatitis A virus in representative food matrices.

    abstract::Hepatitis A virus (HAV) is an important cause of foodborne disease worldwide. The detection of this virus in naturally contaminated food products is complicated by the absence of a reliable culture method, low levels of contamination, and the presence of matrix-associated compounds which inhibit molecular detection. I...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Papafragkou E,Plante M,Mattison K,Bidawid S,Karthikeyan K,Farber JM,Jaykus LA

    更新日期:2008-01-01 00:00:00

  • Real-time TaqMan RT-PCR for detection of maize chlorotic mottle virus in maize seeds.

    abstract::Maize chlorotic mottle virus (MCMV) causes corn lethal necrosis disease, and can be transmitted through infected maize seeds. It remains a challenge to detect this virus in the seeds to prevent its introduction and infection. For this purpose, a real-time TaqMan RT-PCR procedure for efficient detection of MCMV was dev...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Zhang Y,Zhao W,Li M,Chen H,Zhu S,Fan Z

    更新日期:2011-01-01 00:00:00

  • Detection and typing of human herpesviruses by multiplex polymerase chain reaction.

    abstract::A new approach to simultaneous detection and typing of related agents by the multiplex polymerase chain reaction (PCR) is described. The reaction was been applied to human herpesviruses by nested amplification of fragments of the DNA polymerase genes. During the first amplification, primers were used as two equimolar ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Tenorio A,Echevarría JE,Casas I,Echevarría JM,Tabarés E

    更新日期:1993-10-01 00:00:00

  • Detection of cytomegaloviral DNA in human milk cells and cell free milk whey by nested PCR.

    abstract::Human cytomegalovirus (HCMV) DNA can be detected in different compartments of human milk. A protocol for the preparation of milk whey free of fat and cells for the detection of human cytomegalovirus (HCMV) by nested PCR is presented. This is based upon the experience of the separation of more than 200 milk specimens o...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Hamprecht K,Vochem M,Baumeister A,Boniek M,Speer CP,Jahn G

    更新日期:1998-02-01 00:00:00

  • Detection and quantification of four viruses in Prunus pollen: Implications for biosecurity.

    abstract::Pollen transmitted viruses require accurate detection and identification to minimize the risk of spread through the global import and export of pollen. Therefore in this study we developed RT-qPCR assays for the detection of Cherry leaf roll virus (CLRV), Prune dwarf virus (PDV), Prunus necrotic ringspot virus (PNRSV)...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Beaver-Kanuya E,Harper SJ

    更新日期:2019-09-01 00:00:00

  • Rapid diagnosis of influenza infection of NP antigen using an immunocapture ELISA test.

    abstract::An immunocapture ELISA test for the diagnosis of human and animal influenza A and/or B is described. A monoclonal anti-nucleoprotein (NP) antibody was used to capture the NP antigen and the captured antigen was detected by an anti-NP polyclonal rabbit antiserum. Compared with the usual diagnostic method by cultivation...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Chomel JJ,Thouvenot D,Onno M,Kaiser C,Gourreau JM,Aymard M

    更新日期:1989-07-01 00:00:00

  • Quantitative detection of dengue 2 virus using fluorogenic RT-PCR based on 3'-noncoding sequence.

    abstract::A fluorescent DNA probe (DV2.P1) specific to the conserved distal 3'-noncoding region (nucleotides 10653-10678) of dengue 2 virus and a pair of flanking primers (DV2.L1 and DV2.U2) were designed to formulate a dengue 2-specific fluorogenic polymerase chain reaction (PCR). In addition, DV2.L1 was also used as a reverse...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Houng HH,Hritz D,Kanesa-thasan N

    更新日期:2000-04-01 00:00:00

  • A multivariate statistical analysis to follow the course of disease after infection of cats with different strains of the feline immunodeficiency virus (FIV).

    abstract::A descriptive multivariate assay is described which is suitable to analyze results of a biological experiment with small sample size but high qualitative and quantitative complexity of variables. This type of assay allows evaluation of multiple variables observed in the course of an experimental virus infection (e.g. ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: de Monte M,Nonnenmacher H,Brignon N,Ullmann M,Martin JP

    更新日期:2002-05-16 00:00:00

  • Development of a real-time TaqMan RT-PCR assay for the detection of H9N2 avian influenza viruses.

    abstract::Avian influenza viruses (AIVs) of the H9N2 subtype are a major economic problem in the poultry industry in Israel. Most field isolates from the last decade differ significantly from H9N2 isolates from Europe and the USA, rendering published detection methods inadequate. This study aimed to develop a real-time TaqMan((...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Ben Shabat M,Meir R,Haddas R,Lapin E,Shkoda I,Raibstein I,Perk S,Davidson I

    更新日期:2010-09-01 00:00:00