Removal of lactate dehydrogenase-elevating virus from human-in-mouse breast tumor xenografts by cell-sorting.


:Lactate dehydrogenase-elevating virus (LDV) can infect transplantable mouse tumors or xenograft tumors in mice through LDV-contaminated mouse biological materials, such as Matrigel, or through mice infected with LDV. LDV infects specifically mouse macrophages and alters immune system and tumor phenotype. The traditional approaches to remove LDV from tumor cells, by transplanting tumors into rats or culturing tumor cells in vitro, are inefficient, labor-intensive and time-consuming. Furthermore, these approaches are not feasible for primary tumor cells that cannot survive tissue culture conditions or that may change phenotype in rats. This study reports that fluorescence-activated cell sorting (FACS) is a simple and efficient approach for purifying living primary human breast tumor cells from LDV(+) mouse stromal cells, which can be completed in a few hours. When purified from Matrigel contaminated LDV(+) tumors, sorted human breast tumor cells, as well as tumors grown from sorted cells, were shown to be LDV-free, as tested by PCR. The results demonstrate that cell sorting is effective, much faster and less likely to alter tumor cell phenotype than traditional methods for removing LDV from xenograft models. This approach may also be used to remove other rodent-specific viruses from models derived from distinct tissues or species with sortable markers, where virus does not replicate in the cells to be purified.


J Virol Methods


Liu H,Bockhorn J,Dalton R,Chang YF,Qian D,Zitzow LA,Clarke MF,Greene GL




Has Abstract


2011-05-01 00:00:00














  • Detection of wild type and deleted latent membrane protein 1 (LMP1) of Epstein-Barr virus in clinical biopsy material.

    abstract::The latent membrane protein 1 (LMP1) of Epstein-Barr virus (EBV) is one of two postulated viral oncogenic proteins. Sequence variations, and in particular a 30 base pair deletion variant called CAO, may define different disease populations. We developed a panel of rat monoclonal antibodies (MAb) specific for the non-w...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Nicholls J,Hahn P,Kremmer E,Fröhlich T,Arnold GJ,Sham J,Kwong D,Grässer FA

    更新日期:2004-03-01 00:00:00

  • HCV core antigen as an alternate test to HCV RNA for assessment of virologic responses to all-oral, interferon-free treatment in HCV genotype 1 infected patients.

    abstract::In light of the advances in HCV therapy, simplification of diagnosis confirmation, pre- treatment diagnostic workup and treatment monitoring is required to ensure broad access to interferon-free therapies. HCV core antigen (HCV cAg) testing is rapid, giving results in approximately 60min, and less expensive than HCV R...

    journal_title:Journal of virological methods

    pub_type: 临床试验,杂志文章


    authors: Rockstroh JK,Feld JJ,Chevaliez S,Cheng K,Wedemeyer H,Sarrazin C,Maasoumy B,Herman C,Hackett J Jr,Cohen DE,Dawson GJ,Cloherty G,Pawlotsky JM

    更新日期:2017-07-01 00:00:00

  • A simple and rapid method of high quantity DNA isolation from cervical scrapes for detection of human papillomavirus infection.

    abstract::Infection with human papillomavirus (HPV) is an important etiological factor in the development of cervical cancer, and detection of the viral genome is of prognostic importance, particularly for preneoplastic lesions. We developed a simple, easy and efficient non-organic method of DNA extraction from cervical scrapes...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Gopalkrishna V,Francis A,Sharma JK,Das BC

    更新日期:1992-01-01 00:00:00

  • Rapid automation of a cell-based assay using a modular approach: case study of a flow-based Varicella Zoster Virus infectivity assay.

    abstract::Vaccine manufacturing requires constant analytical monitoring to ensure reliable quality and a consistent safety profile of the final product. Concentration and bioactivity of active components of the vaccine are key attributes routinely evaluated throughout the manufacturing cycle and for product release and dosage. ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Joelsson D,Gates IV,Pacchione D,Wang CJ,Bennett PS,Zhang Y,McMackin J,Frey T,Brodbeck KC,Baxter H,Barmat SL,Benetti L,Bodmer JL

    更新日期:2010-06-01 00:00:00

  • Analytical performances of simultaneous detection of HIV-1, HIV-2 and hepatitis C- specific antibodies and hepatitis B surface antigen (HBsAg) by multiplex immunochromatographic rapid test with serum samples: A cross-sectional study.

    abstract:BACKGROUND:The HIV/HCV/HBsAg Triplex consists in manually performed, visually interpreted, lateral flow, immunochromatographic rapid diagnostic test simultaneously detecting in 15min human immunodeficiency virus (HIV)-1 and HIV-2 and hepatitis C virus (HCV)- specific antibodies (Ab) (IgG and IgM) and hepatitis B virus ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Robin L,Mboumba Bouassa RS,Nodjikouambaye ZA,Charmant L,Matta M,Simon S,Filali M,Mboup S,Bélec L

    更新日期:2018-03-01 00:00:00

  • A reverse-transcription competitive PCR assay based on chemiluminescence hybridization for detection and quantification of hepatitis C virus RNA.

    abstract::A reverse-transcription competitive PCR (RT-cPCR) combined with chemiluminescence hybridization was designed for the detection and quantitative determination of serum hepatitis C virus (HCV) RNA. The concentration of HCV RNA was calculated based on an external standard curve that was generated by coamplification of in...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Young KC,Chang TT,Hsiao WC,Cheng PN,Chen SH,Jen CM

    更新日期:2002-05-01 00:00:00

  • Duplex SYBR Green I-based real-time PCR assay for the rapid detection of canine kobuvirus and canine astrovirus.

    abstract::A duplex SYBR Green I-based real-time PCR assay was established for the simultaneous detection of canine kobuvirus (CaKoV) and canine astrovirus (CaAstV). This assay can easily distinguish the two viruses according to their different melting temperatures (Tm) of 80 °C for CaKoV and 86.5 °C for CaAstV; other canine ent...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Wang Y,Li Y,Cui Y,Jiang S,Liu H,Wang J,Li Y

    更新日期:2021-01-13 00:00:00

  • Use of the polymerase chain reaction for the sensitive detection of St. Louis encephalitis viral RNA.

    abstract::Polymerase chain reaction (PCR) assays were developed for the detection of RNA from the St. Louis encephalitis (SLE) virus. Using computer-assisted analysis of the MSI-7 strain SLE virus genome, two primer pairs were selected from the capsid-coding and the membrane associated protein-coding genes, and one from the env...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Howe DK,Vodkin MH,Novak RJ,Shope RE,McLaughlin GL

    更新日期:1992-01-01 00:00:00

  • Detection of equine arteritis virus by real-time TaqMan reverse transcription-PCR assay.

    abstract::A one-tube real-time TaqMan reverse transcription-polymerase chain reaction (RT-PCR) assay was developed for the detection of equine arteritis virus (EAV). The test was validated using the seminal plasma and nasal secretions of infected horses that were proven to contain EAV by traditional virus isolation in rabbit ki...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Balasuriya UB,Leutenegger CM,Topol JB,McCollum WH,Timoney PJ,MacLachlan NJ

    更新日期:2002-03-01 00:00:00

  • A simple, rapid and efficient way to obtain infectious clones of potyviruses.

    abstract::The availability of an infectious cDNA clone is a prerequisite for genetic studies on RNA viruses. However, despite important improvement in molecular biology techniques during the last decades, obtaining such clones often remains tedious, time-consuming and rather unpredictable. In the case of potyviruses, cDNA clone...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Desbiez C,Chandeysson C,Lecoq H,Moury B

    更新日期:2012-07-01 00:00:00

  • Inoculation of plants with begomoviruses by particle bombardment without cloning: Using rolling circle amplification of total DNA from infected plants and whiteflies.

    abstract::A new system for inoculation of plants with begomoviral DNA without cloning or the use insect vectors is described. Total DNA extracted from begomovirus-infected plants was amplified by rolling circle amplification (RCA) using the bacteriophage phi29 DNA polymerase, and inoculated to plants by particle bombardment. In...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Guenoune-Gelbart D,Sufrin-Ringwald T,Capobianco H,Gaba V,Polston JE,Lapidot M

    更新日期:2010-09-01 00:00:00

  • Extraction and purification of hepatitis B virus-like M particles from a recombinant Saccharomyces cerevisiae strain using alumina powder.

    abstract::A recombinant hepatitis B surface antigen (HBsAg) has been produced in the yeast Saccharomyces cerevisiae and used as a vaccine against hepatitis B virus (HBV) infection. The present study aimed to optimize the extraction of recombinant virus-like particles (rVLPs) to develop a simple purification procedure based on g...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Hadiji-Abbes N,Martin M,Benzina W,Karray-Hakim H,Gergely C,Gargouri A,Mokdad-Gargouri R

    更新日期:2013-01-01 00:00:00

  • Enzymatic amplification of latent pseudorabies virus nucleic acid sequences.

    abstract::To investigate various aspects of the latency of pseudorabies virus in swine (PRV, suid herpesvirus 1) we developed in vitro nucleic acid amplification methods based upon the polymerase chain reaction. Primers flanking a 156-bp region of the pseudorabies virus gp II gene were annealed to purified PRV DNA as well as DN...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Lokensgard JR,Thawley DG,Molitor TW

    更新日期:1991-09-01 00:00:00

  • Optimization of an in situ hybridization technique for the detection of foot-and-mouth disease virus in bovine tissues using the digoxigenin system.

    abstract::An in situ hybridization technique has been optimised for use on paraffin-embedded sections of tissues collected from cattle infected experimentally with foot-and-mouth disease virus type O1BFS. Tissue was collected 5 days after infection by direct contact. In situ hybridization was carried out using an RNA probe corr...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Woodbury EL,Ilott MC,Brown CC,Salt JS

    更新日期:1995-01-01 00:00:00

  • Development and evaluation of a droplet digital PCR assay for the detection of fowl adenovirus serotypes 4 and 10 in attenuated vaccines.

    abstract::In recent years, there has been an increase in reported cases of fowl adenovirus serotype 4 (FAdV-4) in chickens in China. The use of live attenuated vaccines contaminated with FAdV-4 has been proved to be one of the important causes of massive outbreaks of hydropericardium syndrome. To detect the contamination with F...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Dong G,Meng F,Zhang Y,Cui Z,Lidan H,Chang S,Zhao P

    更新日期:2019-03-01 00:00:00

  • A quantitative duplex PCR technique for measuring amounts of cell-associated Marek's disease virus: differences in two populations of lymphoma cells.

    abstract::A duplex polymerase chain reaction (PCR) was developed to measure Marek's disease virus (MDV) load in two subpopulations of Marek's disease (MD) lymphoma cells from chickens. PCR primers were designed using the sequence of the MDV-ICP4 gene and the chicken IFNgamma gene. Each set of primers was present in the same rea...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Burgess SC,Davison TF

    更新日期:1999-09-01 00:00:00

  • Multiple primer pairs polymerase chain reaction for the detection of human papillomavirus types.

    abstract::A polymerase chain reaction (PCR) based on the use of multiple primers enabling the simultaneous detection of HPV-6b, -11, -16 and -18 in a single tube reaction was developed and validated on cervico-vaginal specimens, including tissues embedded in paraffin. This PCR setting proved to be specific and sensitive, allowi...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Anceschi MM,Falcinelli C,Pieretti M,Cosmi EV

    更新日期:1990-04-01 00:00:00

  • Concentration and purification of feline leukaemia virus (FeLV) and its outer envelope protein gp70 by aqueous two-phase systems.

    abstract::The major protective antigens of retroviruses are considered to be their glycosylated envelope proteins. However, the methods commonly employed to enrich and purify virus from culture media such as pelleting and density-gradient centrifugation result in a low recovery of the viral external glycoproteins. This is an ob...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Hammar L,Eriksson S,Malm K,Morein B

    更新日期:1989-04-01 00:00:00

  • Real time PCR TaqMan assays for detection of polyomaviruses KIV and WUV in clinical samples.

    abstract::Recently, polyomaviruses KI and WU were identified in the airways of patients with acute respiratory symptoms. The epidemiology and pathogenesis of these two viruses are not fully understood, and the development of molecular assays, such as Real Time PCR, was useful for examining their biology and role in different cl...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Bergallo M,Terlizzi ME,Astegiano S,Ciotti M,Babakir-Mina M,Perno CF,Cavallo R,Costa C

    更新日期:2009-12-01 00:00:00

  • Identification of cell lines permissive for human coronavirus NL63.

    abstract::Six cell lines routinely used in laboratories were tested for permissiveness to the infection with the newly identified human coronavirus NL63. Two monkey epithelial cell lines, LLC-MK2 and Vero-B4, showed a cytopathic effect (CPE) and clear viral replication, whereas no CPE or replication was observed in human lung f...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Schildgen O,Jebbink MF,de Vries M,Pyrc K,Dijkman R,Simon A,Müller A,Kupfer B,van der Hoek L

    更新日期:2006-12-01 00:00:00

  • HPV antibody detection by ELISA with capsid protein L1 fused to glutathione S-transferase.

    abstract::An alternative enzyme linked immunosorbent assay (ELISA) system was developed to analyze antibodies to human papillomavirus capsid antigens. The assay uses glutathione crosslinked to casein to capture the major capsid protein L1 from human papillomavirus (HPV) types 6b, 16 and 18 fused to glutathione S-transferase (GS...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Sehr P,Müller M,Höpfl R,Widschwendter A,Pawlita M

    更新日期:2002-10-01 00:00:00

  • High-throughput real-time PCR for early detection and quantitation of arenavirus Tacaribe.

    abstract::Arenaviruses merit significant attention both as causative agents of endemic hemorrhagic fevers and as model systems to study the immune response to acute and persistent viral infections. Development of highly sensitive quantitative screening methods to detect arenavirus is critical for early diagnosis of patients, to...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Grajkowska LT,Pedras-Vasconcelos JA,Sauder C,Verthelyi D,Puig M

    更新日期:2009-08-01 00:00:00

  • Detection of feline Coronavirus in effusions of cats with and without feline infectious peritonitis using loop-mediated isothermal amplification.

    abstract::Feline infectious peritonitis (FIP) is a fatal disease in cats worldwide. The aim of this study was to test two commercially available reaction mixtures in a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay to detect feline Coronavirus (FCoV) in body cavity effusions of cats with and withou...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Günther S,Felten S,Wess G,Hartmann K,Weber K

    更新日期:2018-06-01 00:00:00

  • Development and validation of a real-time RT-PCR assay for generic detection of pospiviroids.

    abstract::In many countries phytosanitary regulations apply to Potato spindle tuber viroid, because it can cause serious diseases in potato and tomato crops. Other pospiviroids, some of which are distributed widely in ornamental crops, can cause similar diseases. Consequently, there is a need for a reliable and cost-effective g...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Botermans M,van de Vossenberg BT,Verhoeven JT,Roenhorst JW,Hooftman M,Dekter R,Meekes ET

    更新日期:2013-01-01 00:00:00

  • Detection of porcine parvovirus by loop-mediated isothermal amplification.

    abstract::Loop-mediated isothermal amplification is a novel method for rapid amplification of DNA. It has been adopted widely for the detection of virus because of its simplicity, rapidity, and specificity. A loop-mediated isothermal amplification assay was developed for the detection of porcine parvovirus. Four primers specifi...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Chen CM,Cui SJ

    更新日期:2009-02-01 00:00:00

  • Rapid and accurate identification of poliovirus strains used for vaccine production.

    abstract::In the context of eradication of poliomyelitis the World Health Organization stimulates the development of inactivated polio vaccines based on attenuated virus strains. In addition to vaccine development, tests have to be designed to assess the vaccine quality. An important test is the identification test for poliovir...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Nijst OE,Mouthaan JJ,Mekkes DR,Jusic E,van der Avoort HG,Metz B

    更新日期:2013-04-01 00:00:00

  • Solid-phase enzyme immunoassay for rotavirus antigen: faecal protease activity as a reason for false-negative results.

    abstract::Rabbits and guinea pigs were immunized with purified bovine rotavirus. Immunoglobulin G fractions of the resulting antisera were used in a standard four-layer solid-phase enzyme immunoassay (EIA) for rotavirus antigen in human faecal specimens. Samples negative for rotavirus in electron microscopy, when diluted in sta...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Hovi T,Väisänen V,Ukkonen P,von Bonsdorff CH

    更新日期:1982-09-01 00:00:00

  • Chemical inactivation of hepatitis B virus: the effect of disinfectants on virus-associated DNA polymerase activity, morphology and infectivity.

    abstract::The inactivation of hepatitis B virus (HBV) using two commercially available disinfectants was analysed. Indirect evidence of virus inactivation was obtained by examining the decrease in HBV-associated DNA polymerase and HBcAg activities after treatment with increasing concentrations of disinfectant. Inactivation was ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Howard CR,Dixon J,Young P,van Eerd P,Schellekens H

    更新日期:1983-09-01 00:00:00

  • Development and validation of a novel SYBR Green real-time RT-PCR assay for the detection of classical swine fever virus evaluated on different real-time PCR platforms.

    abstract::Classical swine fever is a highly contagious viral disease that causes significant economic losses in pig production on a global scale. The rapid dissemination of the virus and the variability of the clinical signs merit the development of swift and accurate classical swine fever virus (CSFV) detection methods, which ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Pérez LJ,Díaz de Arce H,Tarradas J,Rosell R,Perera CL,Muñoz M,Frías MT,Nuñez JI,Ganges L

    更新日期:2011-06-01 00:00:00

  • Optimization of allele-specific PCR using patient-specific HIV consensus sequences for primer design.

    abstract::Allele-specific PCR based on subtype consensus sequences is a powerful technique for detecting low frequency drug resistant mutants in HIV-1 infected patients. However, this approach can be limited by genetic variation in the region complementary to the primers, leading to variability in allele detection. The goals of...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Boltz VF,Maldarelli F,Martinson N,Morris L,McIntyre JA,Gray G,Hopley MJ,Kimura T,Mayers DL,Robinson P,Mellors JW,Coffin JM,Palmer SE

    更新日期:2010-03-01 00:00:00