Abstract：
:The ongoing Zika virus (ZIKV) outbreak has rapidly spread to new areas of Americas, which were the first transmissions outside its traditional endemic areas in Africa and Asia. Due to the link with newborn defects and neurological disorder, numerous infected cases throughout the world and various mosquito vectors, the virus has been considered to be an international public health emergency. In the present study, we developed a SYBR Green based one-step real-time RT-PCR assay for rapid detection of ZIKV. Our results revealed that the real-time assay is highly specific and sensitive in detection of ZIKV in cell samples. Importantly, the replication of ZIKV at different time points in infected cells could be rapidly monitored by the real-time RT-PCR assay. Specifically, the real-time RT-PCR showed acceptable performance in measurement of infectious ZIKV RNA. This assay could detect ZIKV at a titer as low as 1PFU/mL. The real-time RT-PCR assay could be a useful tool for further virology surveillance and diagnosis of ZIKV.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Xu MY,Liu SQ,Deng CL,Zhang QY,Zhang Bdoi
10.1016/j.jviromet.2016.07.014subject
Has Abstractpub_date
2016-10-01 00:00:00pages
93-97eissn
0166-0934issn
1879-0984pii
S0166-0934(16)30243-9journal_volume
236pub_type
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