Abstract：
:In the context of early infant diagnosis (EID) decentralization in sub-Saharan Africa, dried blood spot (DBS) is now widely used for HIV proviral DNA detection in resource-limited settings. A new version of CAP/CTM (version 2) has been introduced, recently by Roche Diagnosis as a new real-time PCR assay to replace previous technologies on qualitative detection of HIV-1 DNA using whole blood and DBS samples. The objective of this study was to evaluate CAP/CTM version 2 compared to CAP/CTM version 1 and Amplicor on DBS. A total of 261 DBS were collected from children aged 4 weeks to 17 months born from HIV-seropositive mothers and tested by the three techniques. CAP/CTM version 2 showed 100% of agreement with Amplicor including 74 positive results and 187 negative results. CAP/CTM version 2 versus CAP/CTM version 1 as well as CAP/CTM version 1 versus Amplicor showed two discordant results giving a sensitivity of 98.6%, specificity of 99.5%, positive predictive value of 98.6% and negative predictive value of 99.5%. The concordance was 99.12% (95% of confidence interval) giving a Kappa coefficient of 0.97 (p<0.001). These findings confirmed the expected good performance of CAP/CTM version 2 for HIV-1 EID.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Gueye SB,Diop-Ndiaye H,Diallo MM,Ly O,Sow-Ndoye A,Diagne-Gueye ND,Kébé-Fall K,Diop F,Gaye-Diallo A,Belec L,Mboup S,Touré-Kane Cdoi
10.1016/j.jviromet.2015.12.006subject
Has Abstractpub_date
2016-03-01 00:00:00pages
12-5eissn
0166-0934issn
1879-0984pii
S0166-0934(15)00390-0journal_volume
229pub_type
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