Abstract：
:Dengue remains a global public health threat and development of a safe and effective vaccine is a principal public health goal. The primary correlate of immunity is thought to be neutralizing antibodies. Currently, the plaque reduction neutralization test (PRNT) is the gold standard measure of dengue neutralizing antibody responses, but this test is limited by time-consuming performance. In addition, some feel that use of viral strains adapted to grow in Vero or BHK cells may not accurately reflect protective responses. A human cell line transfected to express a putative natural dengue receptor, DC-SIGN (CD209), was used to measure antibody-mediated dengue neutralization. Using neutralizing monoclonal antibodies, immune sera, and laboratory adapted dengue viruses, serotype-specific neutralizing activity was demonstrated similar to that seen in the Vero PRNT. Importantly, serotype-specific neutralizing activity against recently isolated dengue strains with less heterotypic cross-neutralization than laboratory adapted viruses was also demonstrated.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Martin NC,Pardo J,Simmons M,Tjaden JA,Widjaja S,Marovich MA,Sun W,Porter KR,Burgess THdoi
10.1016/j.jviromet.2005.12.002subject
Has Abstractpub_date
2006-06-01 00:00:00pages
74-85issue
1-2eissn
0166-0934issn
1879-0984pii
S0166-0934(05)00409-Xjournal_volume
134pub_type
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journal_title：Journal of virological methods
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