Abstract：
:In March/April 2009, Mexico experienced an outbreak of respiratory illness, due to a new influenza of swine origin virus, which spread rapidly via human-to-human transmission, and became pandemic (A/H1N1pdm). Because of its unique genome composition, which includes gene segments of swine, avian and human origin, and to the considerable differences to the human influenza A viruses that have circulated so far, the currently used molecular methods proved inadequate. Based on published sequences, a primer set targeting the nucleoprotein gene was designed, which provided enhanced sensitivity for the new strain and proved suitable for sequence-based strain identification. The novel nucleoprotein reverse-transcription-PCR showed higher sensitivity for A/H1N1pdm than a commercial test for influenza A, and was comparable to the real-time-based method developed by the Centers for Disease Control and Prevention. It was used to screen 177 clinical samples referred to the laboratory for suspected A/H1N1pdm infection, detecting 17 (9.6%) infections that were confirmed by sequence analysis (100% sensitivity as compared to the real-time kit). The novel method is suitable for the diagnosis of A/H1N1pdm, and is also suitable, at least in the screening phase, for laboratories not equipped with the real-time PCR technology.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Lalle E,Bordi L,Castilletti C,Meschi S,Selleri M,Carletti F,Lapa D,Travaglini D,Ippolito G,Capobianchi MR,Di Caro Adoi
10.1016/j.jviromet.2009.10.004subject
Has Abstractpub_date
2010-02-01 00:00:00pages
486-8issue
2eissn
0166-0934issn
1879-0984pii
S0166-0934(09)00443-1journal_volume
163pub_type
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