One-step reverse transcription loop mediated isothermal amplification assay for sensitive and rapid detection of Cucurbit chlorotic yellows virus.

abstract：:An efficient method for isolation of virus mutants with antigenically altered proteins is described. The method is based on the separation of viruses with wild-type and antigenically altered proteins by affinity chromatography using monoclonal antibodies (MAbs). A nonessential glycoprotein E (gE) of Aujeszky's disease...

journal_title：Journal of virological methods

authors： Morenkov OS,Smirnov SV,Vrublevskaya VV

更新日期：1999-01-01 00:00:00

abstract：:Human cytomegalovirus (HCMV) genome manipulation has always been difficult. Recently, the introduction of full-length HCMV DNA into Escherichia coli as an artificial bacterial chromosome (BAC) clone has allowed reliable targeted mutagenesis. Here, we show the next generation of improvement in designing recombinant HCM...

journal_title：Journal of virological methods

authors： Wang W,Patterson CE,Yang S,Zhu H

更新日期：2004-11-01 00:00:00

abstract：:The genomic DNA molecule of tomato yellow leaf curl virus (TYLCV), a whitefly-transmitted geminivirus, was amplified from total DNA extracts of TYLCV-infected tomato (Lycopersicon esculentum) by the use of loop-mediated isothermal amplification (LAMP). The procedure was also used to amplify TYLCV DNA from total DNA ex...

journal_title：Journal of virological methods

authors： Fukuta S,Kato S,Yoshida K,Mizukami Y,Ishida A,Ueda J,Kanbe M,Ishimoto Y

更新日期：2003-09-01 00:00:00

abstract：:Purified hepatitis B surface antigen (HBsAg) of subtype ay was solubilized in guanidinium chloride and submitted to chromatography on Sepharose 4B in the presence of guanidinium chloride. The polypeptides P1 (Mr = 24,000) and P2 (Mr = 29,000) were eluted in the same fraction with a minor contaminant (Mr = 40,000). Lar...

journal_title：Journal of virological methods

authors： Hubert M,Barin F,Goudeau A

更新日期：1982-01-01 00:00:00

abstract：:One of the most interesting aspects of real-time PCR based on the detection of fluorophoric labeled oligonucleotides is the possibility of being able to detect conveniently multiple targets in the same PCR reaction. Recently, Roche Diagnostics launched a real-time PCR platform, the LightCycler480 (LC480), which should...

journal_title：Journal of virological methods

authors： Molenkamp R,van der Ham A,Schinkel J,Beld M

更新日期：2007-05-01 00:00:00

abstract：:Nine commercial EIAs for measles-specific IgG were compared with haemagglutination inhibition (HI) and plaque reduction neutralization (PRN). A total of 174 sera selected, to give approximately half of the sera without measles antibody by HI, were tested by all EIAs and HI. However, there was sufficient volume of only...

journal_title：Journal of virological methods

authors： Hesketh L,Charlett A,Farrington P,Miller E,Forsey T,Morgan-Capner P

更新日期：1997-06-01 00:00:00

abstract：:HIV-1 RNA viral load has become the major biological marker for disease prognosis and outcome of antiretroviral therapy in the treatment of HIV-infected individuals. The aim of this study was to compare the performance of the new CE marked NucliSens EasyQ HIV-1 assay with NucliSens HIV-1 QT assay (reference method). N...

journal_title：Journal of virological methods

authors： Yao J,Liu Z,Ko LS,Pan G,Jiang Y

更新日期：2005-10-01 00:00:00

abstract：:In the context of eradication of poliomyelitis the World Health Organization stimulates the development of inactivated polio vaccines based on attenuated virus strains. In addition to vaccine development, tests have to be designed to assess the vaccine quality. An important test is the identification test for poliovir...

journal_title：Journal of virological methods

authors： Nijst OE,Mouthaan JJ,Mekkes DR,Jusic E,van der Avoort HG,Metz B

更新日期：2013-04-01 00:00:00

abstract：:Prunus necrotic ringspot ilarvirus (PNRSV) exists as a number of biologically distinct variants which differ in host specificity, serology, and pathology. Previous nucleotide sequence alignment and phylogenetic analysis of cloned reverse transcription-polymerase chain reaction (RT-PCR) products of several biologically...

journal_title：Journal of virological methods

authors： Hammond RW,Crosslin JM,Pasini R,Howell WE,Mink GI

更新日期：1999-07-01 00:00:00

abstract：:E(rns) is an envelope glycoprotein of classical swine fever virus (CSFV) with RNase activity. The purpose of this study was to produce an active E(rns) for further applications using the yeast secreted expression system. The E(rns) gene was cloned into the expression vector pGAPZalphaC which was introduced into Pichia...

journal_title：Journal of virological methods

authors： Huang C,Chien MS,Hu CM,Chen CW,Hsieh PC

更新日期：2006-03-01 00:00:00

abstract：:The aim of this study was the development of gag and pol dual labelled probe real-time PCR and RT PCR assays to quantify the proviral load and the transcripts of the British Visna/maedi virus EV1 strain. Primers and probes were chosen based on the consensus sequences of gag and pol clones representative of EV1 genetic...

journal_title：Journal of virological methods

authors： Carrozza ML,Mazzei M,Bandecchi P,Fraisier C,Pérez M,Suzan-Monti M,de Andrés D,Amorena B,Rosati S,Andrésdottir V,Lujan L,Pepin M,Blacklaws B,Tolari F,Harkiss GD

更新日期：2010-05-01 00:00:00

abstract：:Activated platelets form transient aggregates with monocytes in circulation and have a half-life of approximately 30-60 min. These complexes are increased in various inflammatory conditions and are an early marker of myocardial infarction. HIV-1 infection is associated with chronic inflammation, and increased CD16⁺ in...

journal_title：Journal of virological methods

authors： Singh MV,Davidson DC,Kiebala M,Maggirwar SB

更新日期：2012-05-01 00:00:00

abstract：:A colloid gold strip (CGS) test for detecting antibodies to H5 subtype Avian Influenza Virus (AIV) was developed. The test is based on membrane chromatography and uses colloidal gold conjugated with inactivated AIV-H5N1 as the tracer. On the test strip, a baculovirus expressed haemagglutinin (HA) protein was used as t...

journal_title：Journal of virological methods

authors： Cui S,Chen C,Tong G

更新日期：2008-09-01 00:00:00

abstract：:Recombinant plasmids carrying 199 base pairs (bp) inserts from the non coding region (nucleotides (nt) 6-204) of the TT virus (TTV) genome were used to standardize an heteroduplex mobility assay able to detect mixed infections of a single individual with several TTV isolates. In this simplified heteroduplex mobility a...

journal_title：Journal of virological methods

authors： Saback FL,Gomes SA,Niel C

更新日期：2002-03-01 00:00:00

abstract：:Human cosaviruses (HCoSVs) are newly discovered viruses in Picornaviridae family. Until now, most published studies reported HCoSV detection using molecular techniques and genetic characterization of the virus. Nevertheless, no laboratory has yet reported the replication of these viruses in cultured cell lines. In the...

journal_title：Journal of virological methods

authors： Rezig D,Touzi H,Meddeb Z,Triki H

更新日期：2014-10-01 00:00:00

abstract：:A quantitative and simple indirect radioimmunoassay (IRIA) was developed for porcine parvovirus (PPV), employing a monoclonal antibody directed against PPV adsorbed to nitrocellulose membrane. The IRIA was equally sensitive to live or inactivated PPV. There was a linear relationship between membrane-bound radioactivit...

journal_title：Journal of virological methods

authors： Katz JB,van Deusen RA

更新日期：1985-12-01 00:00:00

abstract：:Using a multiplexed LNA-based Taqman assay, RT-digital PCR (RT-dPCR) was performed in a prefabricated microfluidic device that monitored absolute viral load in native and immortalized cell lines, overall precision of detection, and the absolute detection limit of an occult RNA virus GB Virus Type C (GBV-C). RT-dPCR ha...

journal_title：Journal of virological methods

authors： White RA 3rd,Quake SR,Curr K

更新日期：2012-01-01 00:00:00

abstract：:The use of adenovirus type 35 (Ad35) as a vector in vaccine and gene therapy studies is promising due to its broad cell tropism and low seroprevalence in humans. However, to date, a simple and effective system for producing recombinant Ad35 (rAd35) has not been well developed. This report describes a two-plasmid Ad35-...

journal_title：Journal of virological methods

authors： Wu C,Lei X,Wang J,Hung T

更新日期：2011-10-01 00:00:00

abstract：:Diagnostic tests are usually evaluated in terms of simple qualitative measures of sensitivity and specificity. When comparing different quantitative assays such as ELISAs, it is often more useful to deal with actual values (sample optical density/cut-off optical density ratio (OD ratio] rather than the qualitative rel...

journal_title：Journal of virological methods

authors： Crofts N,Maskill W,Gust ID

更新日期：1988-10-01 00:00:00

abstract：:A solid-phase enzyme-linked immunoassay using a fluorogenic substrate (4-methylumbelliferyl-beta-D-galactopyranoside) was developed. Antibodies were covalently linked to glutaraldehyde-activated 96-well aminopolystyrene plates. Antigens from test samples were adsorbed to the solid phase and detected using antibodies c...

journal_title：Journal of virological methods

authors： Neurath AR,Strick N

更新日期：1981-10-01 00:00:00

abstract：:A novel and rapid EBNA-1 titration method has been developed which uses immunoprecipitation of specific DNA-protein complexes with EBNA-1-positive serum. The method is more sensitive than the conventional immunofluorescence method and has potential value as a diagnostic reagent for clinical laboratories. TPA induction...

journal_title：Journal of virological methods

authors： Nonoyama M,Wen LT,Tabata T,Tanaka A

更新日期：1988-09-01 00:00:00

abstract：:Sequences of hepatitis B virus DNA were detected specifically in the sera of infected individuals by a non-radioactive riboprobe nucleic acid hybridization assay. The nucleic acids contained in serum specimens were prepared by an overnight proteinase K-/SDS-digest, phenol/chloroform-extraction and ethanol-precipitatio...

journal_title：Journal of virological methods

authors： Gerritzen A,Scholt B

更新日期：1990-12-01 00:00:00

abstract：:A rapid and sensitive method for detecting HIV-1 DNA sequences amplified by polymerase chain reaction (PCR) is described. The method uses solution phase hybridization for rapid and efficient annealing between digoxigenin-labeled targets and biotinylated capture probes. Hybrids containing biotin are captured onto strep...

journal_title：Journal of virological methods

authors： Suzuki K,Okamoto N,Watanabe S,Kano T

更新日期：1992-07-01 00:00:00

abstract：:TaqMan Mismatch Amplification Mutation Assay (TaqMAMA) is a highly sensitive allelic discrimination method. The mismatch amplification mutation assay (MAMA) is based on preferential amplification of mutant allele by the 'MAMA' primer, which is designed to have two mismatches with the wild-type allele and only one mism...

journal_title：Journal of virological methods

authors： Curry S,Qiu P,Tong X

更新日期：2008-11-01 00:00:00

abstract：:Enterovirus 71 (EV71), the etiologic agent causes outbreaks with significant mortality in young children in Asia and currently there is no vaccine available. In this study, we report a quantitative enzyme linked immunosorbent assay (Q-ELISA) to determine the concentration of the EV71 VP2 antigen. EV71 virus-like parti...

journal_title：Journal of virological methods

authors： Liu CC,Chang HW,Yang G,Chiang JR,Chow YH,Sai IH,Chang JY,Lin SC,Sia C,Hsiao CH,Chou AH,Chong P

更新日期：2011-09-01 00:00:00

abstract：:The availability of whole genome sequencing has contributed to many aspects of dengue research, and its use in dengue virus (DENV) surveillance for early epidemic warning has been proposed. Methods to sequence the genomes of individual dengue serotypes have been described previously, but no single method is known to b...

journal_title：Journal of virological methods

authors： Christenbury JG,Aw PP,Ong SH,Schreiber MJ,Chow A,Gubler DJ,Vasudevan SG,Ooi EE,Hibberd ML

更新日期：2010-10-01 00:00:00

abstract：:One of the most important steps when preparing a live attenuated vaccine is the assessment of the level of attenuation in target animals. It is costly and time consuming as it requires, on each occasion, a large number of susceptible animals and contained accommodation. This study assessed the consistency of the bovin...

journal_title：Journal of virological methods

authors： Franchi P,Mercante MT,Ronchi GF,Armillotta G,Ulisse S,Molini U,Di Ventura M,Lelli R,Savini G,Pini A

更新日期：2008-11-01 00:00:00

abstract：:The polymerase chain reaction (PCR) methods enable the detection of large number of human papillomavirus (HPV) genotypes that infect the anogenital tract. In this study, two groups of cervical scrapes with abnormal cytomorphology were analysed. The first group was tested with three sets of consensus primers located wi...

journal_title：Journal of virological methods

authors： Husnjak K,Grce M,Magdić L,Pavelić K

更新日期：2000-08-01 00:00:00

abstract：:Peptides of HIV sequences are significant for antibody screening systems, and because of the limited number of amino acids they have to represent immunodominant regions of the virus proteins in order to maintain sensitivity. We detected, in a region of the outside loop of the transmembrane protein gp41 of the human im...

journal_title：Journal of virological methods

authors： Döpel SH,Porstmann T,Henklein P,von Baehr R

更新日期：1989-08-01 00:00:00

abstract：:Indicator cell lines are useful biological tools for monitoring virus infection. In order to monitor infection with bovine immunodeficiency virus (BIV) in vitro, an indicator cell line derived from baby hamster kidney cells which contains integrated copies of an enhanced green fluorescent protein gene driven by the BI...

journal_title：Journal of virological methods

authors： Yao X,Su Y,Liu C,Tan J,Liu L,Geng YQ,Qiao WT

更新日期：2010-01-01 00:00:00