Abstract:
:Purified hepatitis B surface antigen (HBsAg) of subtype ay was solubilized in guanidinium chloride and submitted to chromatography on Sepharose 4B in the presence of guanidinium chloride. The polypeptides P1 (Mr = 24,000) and P2 (Mr = 29,000) were eluted in the same fraction with a minor contaminant (Mr = 40,000). Large amounts of these two polypeptides were obtained in a single step. This technique which constitutes a method for large-scale purification of the P1 and P2 polypeptides should permit more complete characterization of the P1 and P2 polypeptides and of their antigenic determinants.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Hubert M,Barin F,Goudeau Adoi
10.1016/0166-0934(82)90039-8subject
Has Abstractpub_date
1982-01-01 00:00:00pages
349-54issue
6eissn
0166-0934issn
1879-0984pii
0166-0934(82)90039-8journal_volume
3pub_type
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