当前位置： SCI文献检索 > JOURNAL OF VIROLOGICAL METHODS期刊下所有文献 > Differentiation of closely related but biologically distinct cherry isolates of Prunus necrotic ringspot virus by polymerase chain reaction.

Differentiation of closely related but biologically distinct cherry isolates of Prunus necrotic ringspot virus by polymerase chain reaction.

Abstract：

:Prunus necrotic ringspot ilarvirus (PNRSV) exists as a number of biologically distinct variants which differ in host specificity, serology, and pathology. Previous nucleotide sequence alignment and phylogenetic analysis of cloned reverse transcription-polymerase chain reaction (RT-PCR) products of several biologically distinct sweet cherry isolates revealed correlations between symptom type and the nucleotide and amino acid sequences of the 3a (putative movement protein) and 3b (coat protein) open reading frames. Based upon this analysis, RT-PCR assays have been developed that can identify isolates displaying different symptoms and serotypes. The incorporation of primers in a multiplex PCR protocol permits rapid detection and discrimination among the strains. The results of PCR amplification using type-specific primers that amplify a portion of the coat protein gene demonstrate that the primer-selection procedure developed for PNRSV constitutes a reliable method of viral strain discrimination in cherry for disease control and will also be useful for examining biological diversity within the PNRSV virus group.

journal_name

J Virol Methods

journal_title

Journal of virological methods

authors

Hammond RW,Crosslin JM,Pasini R,Howell WE,Mink GI

doi

10.1016/s0166-0934(99)00051-8

subject

Has Abstract

pub_date

1999-07-01 00:00:00

pages

203-12

issue

2

eissn

0166-0934

issn

1879-0984

pii

S0166093499000518

journal_volume

80

pub_type

杂志文章

相关文献

JOURNAL OF VIROLOGICAL METHODS文献大全
  • The use of differential display to isolate viral genomic sequence for rapid development of PCR-based detection methods. A test case using Taura syndrome virus.

    abstract：:The purpose of this study was to explore the efficacy of using differential display (DD) to isolate viral genomic sequence using tissues from infected organisms so that a PCR procedure to detect the pathogen may be developed rapidly. The model virus used was the Taura syndrome virus (TSV), a ssRNA virus that cause hig...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2004.06.008

    authors： Lu Y,Wang SY,Lotz JM

    更新日期：2004-10-01 00:00:00

  • Evaluation of the cobas® HCV test for quantifying HCV RNA in dried plasma spots collected using the cobas® Plasma Separation Card.

    abstract：OBJECTIVE:This study evaluated the performance of the cobas® hepatitis C virus (HCV) Test for use on the cobas® 6800/8800 Systems for the detection and quantification of HCV RNA collected using the cobas® Plasma Separation Card (PSC) compared with plasma samples. METHODS:Whole EDTA-venous blood was collected from 50 H...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2020.113820

    authors： Marins EG,Krey N,Becker A,Melzer S,Hoppler M

    更新日期：2020-04-01 00:00:00

  • Evaluating SARS-CoV-2 spike and nucleocapsid proteins as targets for antibody detection in severe and mild COVID-19 cases using a Luminex bead-based assay.

    abstract：:Large-scale serosurveillance of severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) will only be possible if serological tests are sufficiently reliable, rapid and affordable. Many assays are either labour-intensive and require specialised facilities (e.g. virus neutralization assays), or are expensive w...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2020.114025

    authors： Mariën J,Ceulemans A,Michiels J,Heyndrickx L,Kerkhof K,Foque N,Widdowson MA,Mortgat L,Duysburgh E,Desombere I,Jansens H,Van Esbroeck M,Ariën KK

    更新日期：2021-02-01 00:00:00

  • Development of a hamster kidney cell line expressing stably T7 RNA polymerase using retroviral gene transfer technology for efficient rescue of infectious foot-and-mouth disease virus.

    abstract：:Reverse genetics systems, with the ability to manipulate viral genomes at the DNA molecular level, are an important platform for study of the assembly and function of viruses. Genome manipulation, such as gene recombination, mosaicism, and mutation may interfere with replication, assembly and release of viruses. An ef...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2008.11.010

    authors： Zheng H,Tian H,Jin Y,Wu J,Shang Y,Yin S,Liu X,Xie Q

    更新日期：2009-03-01 00:00:00

  • Duplex SYBR Green I-based real-time PCR assay for the rapid detection of canine kobuvirus and canine astrovirus.

    abstract：:A duplex SYBR Green I-based real-time PCR assay was established for the simultaneous detection of canine kobuvirus (CaKoV) and canine astrovirus (CaAstV). This assay can easily distinguish the two viruses according to their different melting temperatures (Tm) of 80 °C for CaKoV and 86.5 °C for CaAstV; other canine ent...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2021.114066

    authors： Wang Y,Li Y,Cui Y,Jiang S,Liu H,Wang J,Li Y

    更新日期：2021-01-13 00:00:00

  • Analytical evaluation of the microarray-based FluChip-8G Influenza A+B Assay.

    abstract：BACKGROUND:Influenza causes a significant annual disease burden, with characterization of the infecting virus important in clinical and public health settings. Rapid immunoassays are fast but insensitive, whereas real-time RT-PCR is sensitive but susceptible to genetic mutations and often requires multiple serial assay...

    journal_title：Journal of virological methods

    pub_type： 杂志文章,多中心研究

    doi：10.1016/j.jviromet.2019.113686

    authors： Taylor AW,Dawson ED,Blair RH,Johnson JE Jr,Slinskey AH,Smolak AW,Toth E,Liikanen K,Stoughton RS,Smith C,Talbot S,Rowlen KL

    更新日期：2019-11-01 00:00:00

  • Enzyme-linked immunosorbent assay for IgG and IgM antibodies against human parvovirus B19: use of monoclonal antibodies and viral antigen propagated in vitro.

    abstract：:Enzyme-linked immunosorbent assay (ELISA) systems for serum IgG and IgM antibodies to human parvovirus B19 were established by utilizing anti-B19 monoclonal antibodies (mAbs) and human plasma B19 antigen. The specificities of IgG and IgM ELISA were confirmed by indirect immunofluorescence staining and Western blot imm...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(89)90146-8

    authors： Yaegashi N,Shiraishi H,Tada K,Yajima A,Sugamura K

    更新日期：1989-11-01 00:00:00

  • Madin-Darby bovine kidney (MDBK) cells are a suitable cell line for the propagation and study of the bovine poxvirus lumpy skin disease virus.

    abstract：:Lumpy skin disease virus (LSDV) is a poxvirus that causes systemic disease in cattle, resulting in substantial economic loss to affected communities. LSDV is a rapidly emerging pathogen of growing global concern that recently spread from Africa and the Middle East into Europe and Asia, impacting the cattle population ...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2020.113943

    authors： Fay PC,Cook CG,Wijesiriwardana N,Tore G,Comtet L,Carpentier A,Shih B,Freimanis G,Haga IR,Beard PM

    更新日期：2020-11-01 00:00:00

  • A real-time PCR approach based on SPF10 primers and the INNO-LiPA HPV Genotyping Extra assay for the detection and typing of human papillomavirus.

    abstract：:The SPF10 PCR targets a conserved 65bp region of the HPV L1 gene for broad-spectrum amplification. The LiPA assay allows subsequent genotyping of the HPV amplicons. This study aims to develop a SPF10 real-time PCR to achieve simultaneous amplification and detection of the HPV target. That way, LiPA analysis of the HPV...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2012.09.013

    authors： Micalessi MI,Boulet GA,Vorsters A,De Wit K,Jannes G,Mijs W,Ieven M,Van Damme P,Bogers JJ

    更新日期：2013-01-01 00:00:00

  • Comparison of somatic and F+ coliphage enumeration methods with large volume surface water samples.

    abstract：:Coliphages are alternative fecal indicators that may be suitable surrogates for viral pathogens, but majority of standard detection methods utilize insufficient volumes for routine detection in environmental waters. We compared three somatic and F+ coliphage methods based on a paired measurement from 1 L samples colle...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2018.08.007

    authors： McMinn BR,Rhodes ER,Huff EM,Wanjugi P,Ware MM,Nappier SP,Cyterski M,Shanks OC,Oshima K,Korajkic A

    更新日期：2018-11-01 00:00:00

  • New Coxsackievirus 2Apro and 3Cpro protease antibodies for virus detection and discovery of pathogenic mechanisms.

    abstract：:Enteroviruses (EVs), such as the Coxsackie B-viruses (CVBs), are common human pathogens, which can cause severe diseases including meningitis, myocarditis and neonatal sepsis. EVs encode two proteases (2Apro and 3Cpro), which perform the proteolytic cleavage of the CVB polyprotein and also cleave host cell proteins to...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2018.02.001

    authors： Laitinen OH,Svedin E,Kapell S,Hankaniemi MM,Larsson PG,Domsgen E,Stone VM,Määttä JAE,Hyöty H,Hytönen VP,Flodström-Tullberg M

    更新日期：2018-05-01 00:00:00

  • Epitope-mapping on the Epstein-Barr virus major capsid protein using systematic synthesis of overlapping oligopeptides.

    abstract：:Systematic solid-phase synthesis of all possible overlapping nonapeptides of the 1381 amino acid sequence of the Epstein-Barr virus major capsid protein (EBV-MCP) was used to identify the position of linear antigen epitopes on this protein as recognised by human polyclonal antisera. Antisera were selected for reactivi...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(88)90061-4

    authors： Middeldorp JM,Meloen RH

    更新日期：1988-09-01 00:00:00

  • A flow cytometry-based immuno-titration assay for rapid and accurate titer determination of modified vaccinia Ankara virus vectors.

    abstract：:A flow cytometry-based immuno-titration titer assay was established to determine infectious unit (IU) and transducing unit (TU) of modified vaccinia Ankara (MVA) virus vectors. This titration method enumerates infected cells by measuring the expression of viral protein for IU and transgene protein for TU in individual...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2010.07.003

    authors： Li Z,Ling L,Liu X,Laus R,Delcayre A

    更新日期：2010-10-01 00:00:00

  • Coupling generation of cytomegalovirus deletion mutants and amplification of viral BAC clones.

    abstract：:Human cytomegalovirus (HCMV) genome manipulation has always been difficult. Recently, the introduction of full-length HCMV DNA into Escherichia coli as an artificial bacterial chromosome (BAC) clone has allowed reliable targeted mutagenesis. Here, we show the next generation of improvement in designing recombinant HCM...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2004.06.009

    authors： Wang W,Patterson CE,Yang S,Zhu H

    更新日期：2004-11-01 00:00:00

  • Disinfection protocols for necropsy equipment in rabies laboratories: Safety of personnel and diagnostic outcome.

    abstract：:In the last decades, molecular techniques have gradually been adopted for the rapid confirmation of results obtained through gold standard methods. However, international organisations discourage their use in routine laboratory investigations for rabies post-mortem diagnosis, as they may lead to false positive results...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2016.03.017

    authors： Aiello R,Zecchin B,Tiozzo Caenazzo S,Cattoli G,De Benedictis P

    更新日期：2016-08-01 00:00:00

  • Quantitative detection of HIV-1 RNA using NucliSens EasyQ HIV-1 assay.

    abstract：:HIV-1 RNA viral load has become the major biological marker for disease prognosis and outcome of antiretroviral therapy in the treatment of HIV-infected individuals. The aim of this study was to compare the performance of the new CE marked NucliSens EasyQ HIV-1 assay with NucliSens HIV-1 QT assay (reference method). N...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2005.04.017

    authors： Yao J,Liu Z,Ko LS,Pan G,Jiang Y

    更新日期：2005-10-01 00:00:00

  • Improvement of Barley yellow dwarf virus-PAV detection in single aphids using a fluorescent real time RT-PCR.

    abstract：:One of the major factors determining the incidence of Barley yellow dwarf virus (BYDV) on autumn-sown cereals is the viruliferous state of immigrant winged aphids. This variable is assessed routinely using the enzyme-linked immunosorbant assay (ELISA). However, the threshold for virus detection by ELISA can lead to fa...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/s0166-0934(03)00097-1

    authors： Fabre F,Kervarrec C,Mieuzet L,Riault G,Vialatte A,Jacquot E

    更新日期：2003-06-09 00:00:00

  • The use of collagenase to improve the detection of plant viruses in vector nematodes by RT-PCR.

    abstract：:Tomato ringspot virus (ToRSV), Tobacco ringspot virus (TRSV) and Tobacco rattle virus (TRV) are transmitted to healthy plants by viruliferous nematodes in the soil. A method was developed for extraction of genomic viral RNA from virus particles carried within nematodes and a sensitive nested RT-PCR detection assay. Th...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2008.10.002

    authors： Martin RR,Pinkerton JN,Kraus J

    更新日期：2009-01-01 00:00:00

  • TaqMan real time RT-PCR assays for detecting ferret innate and adaptive immune responses.

    abstract：:The ferret is an excellent model for many human infectious diseases including influenza, SARS-CoV, henipavirus and pneumococcal infections. The ferret is also used to study cystic fibrosis and various cancers, as well as reproductive biology and physiology. However, the range of reagents available to measure the ferre...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2014.04.014

    authors： Carolan LA,Butler J,Rockman S,Guarnaccia T,Hurt AC,Reading P,Kelso A,Barr I,Laurie KL

    更新日期：2014-09-01 00:00:00

  • Development and application of a monoclonal antibody against grouper iridovirus (GIV) major capsid protein.

    abstract：:The major capsid protein (MCP) is a main structural protein of iridoviruses, and is used as a marker for the identification, differentiation and classification of ranaviruses. In the present study, six monoclonal antibodies (mAbs) against recombinant MCP of grouper iridovirus (GIV) were produced and characterized. All...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2014.04.013

    authors： Lin HY,Liou CJ,Cheng YH,Hsu HC,Yiu JC,Chiou PP,Lai YS

    更新日期：2014-09-01 00:00:00

  • In vitro stability of Cucumber mosaic virus nanoparticles carrying a Hepatitis C virus-derived epitope under simulated gastrointestinal conditions and in vivo efficacy of an edible vaccine.

    abstract：:The Cucumber mosaic virus (CMV) is an isodiametric plant virus with an extremely wide host range, present worldwide. CMV chimeric particles (R9-CMV), engineered to express a 27-aa synthetic peptide derived from Hepatitis C virus (HCV), were demonstrated to be stable under simulated gastric and intestinal conditions. T...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2010.01.021

    authors： Nuzzaci M,Vitti A,Condelli V,Lanorte MT,Tortorella C,Boscia D,Piazzolla P,Piazzolla G

    更新日期：2010-05-01 00:00:00

  • A reverse-transcription competitive PCR assay based on chemiluminescence hybridization for detection and quantification of hepatitis C virus RNA.

    abstract：:A reverse-transcription competitive PCR (RT-cPCR) combined with chemiluminescence hybridization was designed for the detection and quantitative determination of serum hepatitis C virus (HCV) RNA. The concentration of HCV RNA was calculated based on an external standard curve that was generated by coamplification of in...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/s0166-0934(01)00403-7

    authors： Young KC,Chang TT,Hsiao WC,Cheng PN,Chen SH,Jen CM

    更新日期：2002-05-01 00:00:00

  • Grapevine vitivirus A eradication in Vitis vinifera explants by antiviral drugs and thermotherapy.

    abstract：:Grapevine shoot cultures infected by Grapevine vitivirus A (GVA) were grown on Quorin-Lepoivre basic medium and submitted to in vitro chemotherapy and thermotherapy sanitation techniques. Ribavirin (Rb) at 20gml(-1), dihydroxypropyladenine (DHPA) at 60gml(-1) and their combination (RbDH) were added to the proliferatin...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2007.06.008

    authors： Panattoni A,D'Anna F,Cristani C,Triolo E

    更新日期：2007-12-01 00:00:00

  • Comparison of the Abbott RealTime High Risk HPV test and INNO-LiPA HPV Genotyping Extra test for the detection of human papillomaviruses in formalin-fixed, paraffin-embedded cervical cancer specimens.

    abstract：:Comparative evaluation of Abbott RealTime and Innogenetics INNO-LiPA on alternately processed formalin-fixed, paraffin-embedded specimens of 31 cervical cancers and 31 uterine myomas showed complete agreement in the detection of 14 assay-common HPV genotypes and partial genotyping of HPV-16 and HPV-18. The tissue prep...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2011.04.006

    authors： Kocjan BJ,Seme K,Poljak M

    更新日期：2011-07-01 00:00:00

  • Inferring viral population structures using heteroduplex mobility and DNA sequence analyses.

    abstract：:Heteroduplex mobility (HMA) and tracking assays (HTA) are used to assess genetic relationships between DNA molecules. While distinguishing relationships between clonal or nearly clonal molecules is relatively straightforward, inferring population structures is more complex. To address this issue, HIV-1 quasispecies wi...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2013.08.012

    authors： Shankarappa R,Mullins JI

    更新日期：2013-12-01 00:00:00

  • Detection and typing of human herpesviruses by multiplex polymerase chain reaction.

    abstract：:A new approach to simultaneous detection and typing of related agents by the multiplex polymerase chain reaction (PCR) is described. The reaction was been applied to human herpesviruses by nested amplification of fragments of the DNA polymerase genes. During the first amplification, primers were used as two equimolar ...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(93)90061-u

    authors： Tenorio A,Echevarría JE,Casas I,Echevarría JM,Tabarés E

    更新日期：1993-10-01 00:00:00

  • Detection of rice tungro bacilliform virus in field and glasshouse samples from India using the polymerase chain reaction.

    abstract：:Rice tungro bacilliform virus (RTBV) together with rice tungro spherical virus (RTSV) is the causal agent for the rice tungro disease. A rapid technique was developed to detect RTBV DNA in the crude extract of freshly collected leaf samples by polymerase chain reaction (PCR). This technique can detect the viral DNA in...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(95)01987-1

    authors： Dasgupta I,Das BK,Nath PS,Mukhopadhyay S,Niazi FR,Varma A

    更新日期：1996-04-26 00:00:00

  • Development of chemiluminescent probe hybridization, RT-PCR and nucleic acid cycle sequencing assays of Sabin type 3 isolates to identify base pair 472 Sabin type 3 mutants associated with vaccine associated paralytic poliomyelitis.

    abstract：:Sabin type 3 polio vaccine virus is the most common cause of poliovaccine associated paralytic poliomyelitis. Vaccine associated paralytic poliomyelitis cases have been associated with Sabin type 3 revertants containing a single U to C substitution at bp 472 of Sabin type 3. A rapid method of identification of Sabin t...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/s0166-0934(97)00114-6

    authors： Old MO,Logan LH,Maldonado YA

    更新日期：1997-11-01 00:00:00

  • A solvent-free, rapid and simple virus RNA-release method for potato leafroll virus detection in aphids and plants by reverse transcription polymerase chain reaction.

    abstract：:A one-step, rapid and economical method for potato leafroll virus (PLRV) RNA release that is applicable to the use on a microcentrifuge scale is described. Discs (3-6 mm diameter) from leaves, petioles, stems, and tubers of potato plants were incubated in microcentrifuge tubes with detergent solution. The supernatants...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/s0166-0934(99)00102-0

    authors： Singh RP

    更新日期：1999-12-01 00:00:00

  • Solid phase reverse passive hemadsorption test for hepatitis B surface antigen.

    abstract：:A solid phase reverse passive hemadsorption test (SP-RPHAd) for hepatitis B surface antigen detection is described. It was compared with a commercial reverse passive hemagglutination assay (Hepatest, Wellcome, U.K.). SP-RPHAd is four-fold less expensive than Hepatest and undiluted sera can be used instead of eight-fol...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(87)90115-7

    authors： Duverlie G,Driencourt M,Roussel C,Daniel P

    更新日期：1987-11-01 00:00:00