The use of collagenase to improve the detection of plant viruses in vector nematodes by RT-PCR.


:Tomato ringspot virus (ToRSV), Tobacco ringspot virus (TRSV) and Tobacco rattle virus (TRV) are transmitted to healthy plants by viruliferous nematodes in the soil. A method was developed for extraction of genomic viral RNA from virus particles carried within nematodes and a sensitive nested RT-PCR detection assay. The procedure has been adapted to microscale for handling multiple samples. This assay is effective for detection of ToRSV or TRSV in Xiphinema americanum or TRV in Paratrichodorus allius. With this method, viruses can be detected in nematodes fed on infected plants or from field-collected nematodes where the percentage of viruliferous nematodes is unknown. Soil samples from four red raspberry fields infected with ToRSV were collected in 2003 and 2004. Nematodes isolated from these samples were assayed for ToRSV by RT-PCR and compared to cucumber baiting bioassay for virus transmission from the same soil samples. ToRSV was detected in nematodes throughout the season with similar frequencies by the RT-PCR assay and the transmission bioassay.


J Virol Methods


Martin RR,Pinkerton JN,Kraus J




Has Abstract


2009-01-01 00:00:00














  • Pan-serotypic detection of foot-and-mouth disease virus using a minor groove binder probe reverse transcription polymerase chain reaction assay.

    abstract::A novel assay for the pan-serotypic detection of foot-and-mouth disease virus (FMDV) was designed using a 5' conjugated minor groove binder (MGB) probe real-time RT-PCR system. This assay targets the 3D region of the FMDV genome and is capable of detecting 20 copies of a transcribed RNA standard. The linear range of t...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: McKillen J,McMenamy M,Reid SM,Duffy C,Hjertner B,King DP,Bélak S,Welsh M,Allan G

    更新日期:2011-06-01 00:00:00

  • Direct RT-PCR method for detecting two chrysanthemum viroids using minimal amounts of plant tissue.

    abstract::A direct reverse transcription-polymerase chain reaction (RT-PCR) method for detecting the chrysanthemum stunt viroid (CSVd) and chrysanthemum chlorotic mottle viroid (CChMVd) to screen for a viroid-free chrysanthemum plant at a small plant size was established and named microtissue direct RT-PCR. A razor or syringe n...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Hosokawa M,Matsushita Y,Uchida H,Yazawa S

    更新日期:2006-01-01 00:00:00

  • Primary recovery and chromatographic purification of adeno-associated virus type 2 produced by baculovirus/insect cell system.

    abstract::Adeno-associated virus (AAV) is making its place in gene therapy applications; however, the industry is still facing obstacles in producing a large quantity of highly purified material for clinical studies. Insect cell technology can be used to produce AAV to meet the current demand. During the purification process it...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Chahal PS,Aucoin MG,Kamen A

    更新日期:2007-01-01 00:00:00

  • HCV core antigen as an alternate test to HCV RNA for assessment of virologic responses to all-oral, interferon-free treatment in HCV genotype 1 infected patients.

    abstract::In light of the advances in HCV therapy, simplification of diagnosis confirmation, pre- treatment diagnostic workup and treatment monitoring is required to ensure broad access to interferon-free therapies. HCV core antigen (HCV cAg) testing is rapid, giving results in approximately 60min, and less expensive than HCV R...

    journal_title:Journal of virological methods

    pub_type: 临床试验,杂志文章


    authors: Rockstroh JK,Feld JJ,Chevaliez S,Cheng K,Wedemeyer H,Sarrazin C,Maasoumy B,Herman C,Hackett J Jr,Cohen DE,Dawson GJ,Cloherty G,Pawlotsky JM

    更新日期:2017-07-01 00:00:00

  • User-friendly Taqman probe coupled-insulated isothermal PCR (iiPCR) for rapid detection of emerging Ambystoma tigrinum virus (ATV) in western tiger salamanders (Ambystoma mavortium) on a compact, portable instrument.

    abstract::Portable user-friendly diagnostic tests can benefit detection and surveillance of wildlife diseases. Here, the performance of a compact POCKIT™ Nucleic Acid Analyzer for detection of Ambystoma tigrinum virus (ATV), an emerging Iridovirus that is associated with high host mortality in the western tiger salamander (Amby...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Lung O,Reimer SA,Goater CP

    更新日期:2017-11-01 00:00:00

  • Comparison of two commercial kits and an in-house ELISA for the detection of equine rotavirus in foal feces.

    abstract::Group A rotaviruses (RVA) are important infectious agents associated with diarrhea in the young of several animal species including foals. Currently, a variety of diagnosis methods are commercially available, like ELISA, latex agglutination and immunochromatographic assays. These commercial tests are mainly designed f...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Miño S,Kern A,Barrandeguy M,Parreño V

    更新日期:2015-09-15 00:00:00

  • Development and validation of a novel SYBR Green real-time RT-PCR assay for the detection of classical swine fever virus evaluated on different real-time PCR platforms.

    abstract::Classical swine fever is a highly contagious viral disease that causes significant economic losses in pig production on a global scale. The rapid dissemination of the virus and the variability of the clinical signs merit the development of swift and accurate classical swine fever virus (CSFV) detection methods, which ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Pérez LJ,Díaz de Arce H,Tarradas J,Rosell R,Perera CL,Muñoz M,Frías MT,Nuñez JI,Ganges L

    更新日期:2011-06-01 00:00:00

  • A cost-effective algorithm for the diagnosis of Hepatitis C virus infection and prediction of HCV viremia in Cameroon.

    abstract::Conventional tests for antibody to Hepatitis C virus (HCV) and HCV RNA require considerable time before results are available, remain very expensive and are not adapted to many sub-Saharan African countries where HCV is endemic. The aim of this study was to evaluate the accuracy of an algorithm consisting of two HCV r...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Njouom R,Tejiokem MC,Zanga MC,Pouillot R,Ayouba A,Pasquier C,Nerrienet E

    更新日期:2006-05-01 00:00:00

  • A method for the purification of rotaviruses and adenoviruses from faeces.

    abstract::Rotaviruses and non-cultivable enteric adenoviruses were purified from large volumes of faecal extracts using polyethylene glycol precipitation, centrifugation through 45% w/v sucrose and segregation by density in a cesium chloride density gradient. Yields were high and the preparations were pure enough to use in the ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Beards GM

    更新日期:1982-08-01 00:00:00

  • Competitive polymerase chain reaction assay for quantitation of HIV-1 DNA and RNA.

    abstract::A quantitative PCR assay for the detection of HIV-1 nucleic acids is described. The assay is based on a competitive internal standard nucleic acid which can be discriminated from target sequences by the presence of a new restriction enzyme site. The method was used to quantitate plasmid molecules containing HIV-1 sequ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Stieger M,Démollière C,Ahlborn-Laake L,Mous J

    更新日期:1991-09-01 00:00:00

  • Assaying the activity of HIV-1 integrase with DNA-coated plates.

    abstract::Integration of reverse transcribed viral DNA of HIV into host chromosomes is mediated by the viral enzyme, integrase. This enzymatic activity can be monitored in vitro by integration of a small labeled DNA (donor) into a second unlabeled DNA (target). The methodology usually involves isotope labeling and gel electroph...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Chang YC,Ching TT,Syu WJ

    更新日期:1996-05-01 00:00:00

  • Detection and quantitation of HBV DNA in miniaturized samples: multi centre study to evaluate the performance of the COBAS ® AmpliPrep/COBAS ® TaqMan ® hepatitis B virus (HBV) test v2.0 by the use of plasma or serum specimens.

    abstract::Laboratory analysis of blood specimens is an increasingly important tool for rapid diagnosis and control of therapy. So, miniaturization of test systems is needed, but reduced specimens might impair test quality. For rapid detection and quantitation of HBV DNA, the COBAS(®) AmpliPrep/COBAS(®) TaqMan(®) HBV test has pr...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Berger A,Gohl P,Stürmer M,Rabenau HF,Nauck M,Doerr HW

    更新日期:2010-11-01 00:00:00

  • Development of an EvaGreen-based multiplex real-time PCR assay with melting curve analysis for simultaneous detection and differentiation of six viral pathogens of porcine reproductive and respiratory disorder.

    abstract::Concurrent infection of pigs with two or more pathogens is common in pigs under intensive rearing conditions. Porcine circovirus type 2 (PCV2), porcine parvovirus (PPV), porcine reproductive and respiratory syndrome virus (PRRSV), classical swine fever virus (CSFV), Japanese encephalitis virus (JEV) and pseudorabies v...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Rao P,Wu H,Jiang Y,Opriessnig T,Zheng X,Mo Y,Yang Z

    更新日期:2014-11-01 00:00:00

  • Formation of subviral particles of the capsid protein VP2 of infectious bursal disease virus and its application in serological diagnosis.

    abstract::Infectious bursal disease virus (IBDV) is an immunosuppressive disease of young chicken characterized by severe depletion of B-lymphocytes in the bursa of Fabricius. To provide antigen for diagnostic tests, its major structural protein VP2 was expressed in the yeast Saccharomyces cerevisiae. Electron microscopy of pur...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Dey S,Upadhyay C,Madhan Mohan C,Kataria JM,Vakharia VN

    更新日期:2009-04-01 00:00:00

  • TaqMan real-time and conventional nested PCR tests specific to yellow head virus genotype 7 (YHV7) identified in giant tiger shrimp in Australia.

    abstract::In 2013, a unique seventh yellow head virus genotype (YHV7) was detected in Black Tiger shrimp (Penaeus monodon) broodstock that suffered high mortality following their capture from Joseph Bonaparte Gulf (JBG) in northern Australia. To assist with its diagnosis and assessment of its distribution, prevalence and pathog...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Cowley JA,Rao M,Mohr P,Moody NJ,Sellars MJ,Crane MS

    更新日期:2019-11-01 00:00:00

  • A modified immunofluorescence test for Epstein-Barr virus-specific IgM antibody.

    abstract::The fluorescent antibody (FA) test for Epstein-Barr virus (EBV)-specific IgM antibody was improved by the use of sodium butyrate to induce a higher level of EBV antigen expression in P3HR-1 slide preparations and by removal of rheumatoid factor (RF) and IgG antibodies from test sera by means of adsorption with suspens...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Harnett GB,Palmer CA,Bucens MR

    更新日期:1985-10-01 00:00:00

  • Construction of infectious dengue 2 virus cDNA clones using high copy number plasmid.

    abstract::Procedures for cloning entire dengue serotype 2 virus genome in the multiple cloning site of a commercially available high copy number plasmid are described. The 10.7 kb viral RNA genome was reverse transcribed, amplified as three overlapping DNA fragments and successively ligated into pBluescript II KS, which contain...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Sriburi R,Keelapang P,Duangchinda T,Pruksakorn S,Maneekarn N,Malasit P,Sittisombut N

    更新日期:2001-03-01 00:00:00

  • The development of a real-time reverse transcription-polymerase chain reaction (rRT-PCR) assay using TaqMan technology for the pan detection of bluetongue virus (BTV).

    abstract::Bluetongue virus (BTV) is an infectious, non-contagious viral disease of domestic and wild ruminants that is transmitted by adult females of certain Culicoides species. Since 2006, several serotypes including BTV-1, 2, 4, 6, 8, 9 and 16, have spread from the Mediterranean basin into Northern Europe for the first time....

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Mulholland C,McMenamy MJ,Hoffmann B,Earley B,Markey B,Cassidy J,Allan G,Welsh MD,McKillen J

    更新日期:2017-07-01 00:00:00

  • A quantitative duplex PCR technique for measuring amounts of cell-associated Marek's disease virus: differences in two populations of lymphoma cells.

    abstract::A duplex polymerase chain reaction (PCR) was developed to measure Marek's disease virus (MDV) load in two subpopulations of Marek's disease (MD) lymphoma cells from chickens. PCR primers were designed using the sequence of the MDV-ICP4 gene and the chicken IFNgamma gene. Each set of primers was present in the same rea...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Burgess SC,Davison TF

    更新日期:1999-09-01 00:00:00

  • A biotin-avidin-amplified inhibition enzyme immunoassay for detection of CMV antibodies in human serum.

    abstract::In this inhibition immunoassay undiluted serum reacts in solution with crude cellular CMV antigen in wells of microtestplates coated with hyperimmune CMV-reactive monkey IgG. CMV antibodies in the serum under test block (completely or partial) the fixation of antigen to the capture layer. Unblocked antigenic activity ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Nielsen CM,Hansen K,Andersen HM,Gerstoft J,Vestergaard BF

    更新日期:1987-06-01 00:00:00

  • Coupling of foot-and-mouth disease virus to sheep red blood cells using tannic acid for immunological assays.

    abstract::A technique for coupling foot-and-mouth disease virus (FMDV) to tanned sheep red blood cells (SRBC) is reported. Different parameters influencing the procedure were studied. Subtypes C2, C3, O1 and A24 were used as antigens, and guinea pig hyperimmune sera obtained were tested for specific antibody in passive hemagglu...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Pujol CA,Feledi CA,Massouh EJ

    更新日期:1988-08-01 00:00:00

  • Demonstration of calicivirus in human faeces by immunosorbent and immunogold-labelling electron microscopy methods.

    abstract::Immunosorbent electron microscopy (ISEM) and immuno-gold staining (IGS) electron microscopy methods have been applied to human faeces, shown by direct electron microscopy (EM) to contain calicivirus. Caliciviruses were successfully trapped on grids coated with positive rabbit or human antisera against calicivirus, but...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Kjeldsberg E

    更新日期:1986-11-01 00:00:00

  • Optimal transfection methods and comparison of PK-15 and Dulac cells for rescue of chimeric porcine circovirus type 1-2.

    abstract::A chimeric porcine circovirus type 1-2 (PCV1-2) infectious DNA clone has low transfection efficiency and exhibits low levels of proliferation. Electroporation and lipofection parameters were optimized for PK-15 and Dulac cells with the purpose of increasing the efficiency for rescuing infectious PCV1-2. Titers of PCV1...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Li J,Yu T,Zhou J,Tu W,Gao S,Liu X

    更新日期:2014-11-01 00:00:00

  • Characterization of molecular clones of porcine endogenous retrovirus-A containing different numbers of U3 repeat boxes in the long terminal repeat region.

    abstract::When full-length molecular clones of porcine endogenous retrovirus (PERV)-A and porcine endogenous retrovirus (PERV)-B were passaged on human cells, an increase in the length of the long terminal repeat (LTR) was reported. A 39-bp repeat box in the LTR U3 region was multimerized dynamically upon replication, acting as...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Lee YJ,Park SH,Bae EH,Jung YT

    更新日期:2012-04-01 00:00:00

  • Duplex real-time qRT-PCR for the detection of hepatitis A virus in water and raspberries using the MS2 bacteriophage as a process control.

    abstract::Hepatitis A virus (HAV) infection is the leading worldwide cause of acute viral hepatitis. An important aspect of viral control is rapid diagnosis. Epidemiological studies have linked hepatitis A outbreaks to the consumption of drinking water or soft fruits exposed to faecal contamination. Real-time reverse transcript...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Blaise-Boisseau S,Hennechart-Collette C,Guillier L,Perelle S

    更新日期:2010-06-01 00:00:00

  • Recovery of human norovirus from water by virus concentration methods.

    abstract::The aim of this study was to evaluate the applicability of virus concentration methods to detect human norovirus (HuNoV) in water. One conventional virus concentration method using an electropositive filter (1MDS-method) and two methods developed by our research group using an electronegative filter (Mg-method and Al-...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Haramoto E,Katayama H,Utagawa E,Ohgaki S

    更新日期:2009-09-01 00:00:00

  • Detection of Junin virus by the polymerase chain reaction.

    abstract::Argentine hemorrhagic fever is an often fatal human disease caused by Junin virus, an RNA-containing virus and member of the Arenavirus family. This virus was detected in vitro by the polymerase chain reaction (PCR) procedure. A pair of Junin virus-specific PCR DNA oligonucleotide primers and an oligonucleotide probe ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Bockstahler LE,Carney PG,Bushar G,Sagripanti JL

    更新日期:1992-09-01 00:00:00

  • Detection of cytomegaloviral DNA in human milk cells and cell free milk whey by nested PCR.

    abstract::Human cytomegalovirus (HCMV) DNA can be detected in different compartments of human milk. A protocol for the preparation of milk whey free of fat and cells for the detection of human cytomegalovirus (HCMV) by nested PCR is presented. This is based upon the experience of the separation of more than 200 milk specimens o...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Hamprecht K,Vochem M,Baumeister A,Boniek M,Speer CP,Jahn G

    更新日期:1998-02-01 00:00:00

  • Quantification of recombinant core-like particles of bluetongue virus using immunosorbent electron microscopy.

    abstract::Immunosorbent electron microscopy was used to quantify recombinant baculovirus-generated bluetongue virus (BTV) core-like particles (CLP) in either purified preparations or lysates of recombinant baculovirus-infected cells. The capture antibody was an anti-BTV VP7 monoclonal antibody. The CLP concentration in purified...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Zheng YZ,Hyatt A,Wang LF,Eaton BT,Greenfield PF,Reid S

    更新日期:1999-06-01 00:00:00

  • Novel one-step real-time RT-PCR assay for rapid and specific diagnosis of Crimean-Congo hemorrhagic fever encountered in the Balkans.

    abstract::Early and accurate diagnosis of Crimean-Congo hemorrhagic fever (CCHF) is essential for the treatment and outcome of the disease and prevention of its further transmission. Molecular-based diagnostic assays now serve as the front-line tool in the diagnosis of CCHF. However, the development of real-time RT-PCR assay fo...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Duh D,Saksida A,Petrovec M,Dedushaj I,Avsic-Zupanc T

    更新日期:2006-05-01 00:00:00