Detection of rice tungro bacilliform virus in field and glasshouse samples from India using the polymerase chain reaction.

abstract：:Nipah virus, a zoonotic paramyxovirus which emerged recently was chemically inactivated using binary ethylenimine (BEI). The inactivated virus was concentrated and purified by sucrose gradient centrifugation. The gradient fractions were examined by electron microscopy and Western immunoblot, and gradient fraction cont...

journal_title：Journal of virological methods

authors： Berhane Y,Berry JD,Ranadheera C,Marszal P,Nicolas B,Yuan X,Czub M,Weingartl H

更新日期：2006-03-01 00:00:00

abstract：:The humoral immune response of sheep infected with bluetongue virus serotypes 3, 9 and 16 was monitored by plaque inhibition (PI), blocking ELISA (BELISA) and indirect ELISA over a period of 63 days post-infection. Results indicated that testing of a single plasma or serum sample by both a BELISA and an indirect ELISA...

journal_title：Journal of virological methods

authors： Lunt RA,Blacksell SD,Newberry KM

更新日期：1994-06-01 00:00:00

abstract：:Participation in external quality assessment programs is critical to ensure quality clinical laboratory testing. Commercially available proficiency test panels for HIV-1 virus load testing that are used commonly in external quality assessment programs remain a financial obstacle to resource-limited countries. Maintain...

journal_title：Journal of virological methods

authors： Ramos A,Nguyen S,Garcia A,Subbarao S,Nkengasong JN,Ellenberger D

更新日期：2013-03-01 00:00:00

abstract：:Lentiviral vectors (LVs) are promising delivery systems for gene therapy. To enhance the efficiency of target cell transduction by LVs, protocols often include the addition of culture additives. In this study, the cationic amphipathic peptide LAH4-L1 (KKALLAHALHLLALLALHLAHALKKA), a DNA transfection agent, was evaluate...

journal_title：Journal of virological methods

authors： Fenard D,Genries S,Scherman D,Galy A,Martin S,Kichler A

更新日期：2013-05-01 00:00:00

abstract：:A quantitative PCR method was developed in order to quantitate the number of copies of Pseudorabies virus (PRV) genome present in tissues from infected pigs. The method is based on the use of an internal standard that differs from the target DNA by a deletion of ten base pairs, and that is co-amplified with the target...

journal_title：Journal of virological methods

authors： Thiery R,Pannetier C,Rziha HJ,Jestin A

更新日期：1996-09-01 00:00:00

abstract：:Congenital human cytomegalovirus infections are the major infectious cause of birth defects in the United States. How this virus crosses the placenta and causes fetal disease is poorly understood. Guinea pig cytomegalovirus (GPCMV) is a related virus that provides an important model for studying cytomegaloviral congen...

journal_title：Journal of virological methods

authors： Cui X,McGregor A,Schleiss MR,McVoy MA

更新日期：2008-05-01 00:00:00

abstract：:A method for detecting the emergence of potential pandemic-causing influenza strains has been developed. The system first uses real-time RT-PCR to detect H5, the highly pathogenic avian influenza subtype most likely to cause a pandemic. Pyrosequencing is then employed to scan for codon changes encoding amino acids kno...

journal_title：Journal of virological methods

authors： Waybright N,Petrangelo E,Lowary P,Bogan J,Mulholland N

更新日期：2008-12-01 00:00:00

abstract：:The immunoperoxidase and immunoblotting techniques were used to detect measles virus antigens in nasopharyngeal samples taken from children visiting the Yaounde Health Centre (Cameroon). In order to make these diagnostic tests simpler, faster and less costly, peroxidase-labelled anti-measles IgG were prepared and comp...

journal_title：Journal of virological methods

authors： Njayou M,Balla A

更新日期：1990-07-01 00:00:00

abstract：:An immunocapture ELISA test for the diagnosis of human and animal influenza A and/or B is described. A monoclonal anti-nucleoprotein (NP) antibody was used to capture the NP antigen and the captured antigen was detected by an anti-NP polyclonal rabbit antiserum. Compared with the usual diagnostic method by cultivation...

journal_title：Journal of virological methods

authors： Chomel JJ,Thouvenot D,Onno M,Kaiser C,Gourreau JM,Aymard M

更新日期：1989-07-01 00:00:00

abstract：:The purpose of this study was to explore the efficacy of using differential display (DD) to isolate viral genomic sequence using tissues from infected organisms so that a PCR procedure to detect the pathogen may be developed rapidly. The model virus used was the Taura syndrome virus (TSV), a ssRNA virus that cause hig...

journal_title：Journal of virological methods

authors： Lu Y,Wang SY,Lotz JM

更新日期：2004-10-01 00:00:00

abstract：:Influenza vaccines must be revised constantly on almost a yearly basis because of the sequential mutations (antigenic drift) that occur as the virus responds to immunologic pressure. New, high yield (hy) reassortant viruses have proved essential to meet production needs for the supply of new vaccines. We have devised ...

journal_title：Journal of virological methods

authors： Brett I,Werber J,Kilbourne ED

更新日期：2002-02-01 00:00:00

abstract：:One of the most interesting aspects of real-time PCR based on the detection of fluorophoric labeled oligonucleotides is the possibility of being able to detect conveniently multiple targets in the same PCR reaction. Recently, Roche Diagnostics launched a real-time PCR platform, the LightCycler480 (LC480), which should...

journal_title：Journal of virological methods

authors： Molenkamp R,van der Ham A,Schinkel J,Beld M

更新日期：2007-05-01 00:00:00

abstract：:A simple sequencing-based assay is described for genotyping of hepatitis C virus (HCV). RT-PCR was employed to amplify a 237-nucleotide-long fragment from the 5' untranslated region (UTR) of the genome using one biotinylated and one normal primer. Subsequent to capture of the PCR products on streptavidin-coated beads,...

journal_title：Journal of virological methods

authors： Elahi E,Pourmand N,Chaung R,Rofoogaran A,Boisver J,Samimi-Rad K,Davis RW,Ronaghi M

更新日期：2003-05-01 00:00:00

abstract：:Monoclonal antibodies (Mabs) against influenza B virus were obtained by immunizing mice with B/Nagasaki/1/87, one of the strains of the B/Victoria group. Immunoprecipitation analysis revealed that individual Mabs precipitated the nucleoprotein (NP), the matrix protein (M) or the hemagglutinin protein (HA). By using th...

journal_title：Journal of virological methods

authors： Nakagawa N,Maeda A,Kase T,Kubota R,Okuno Y

更新日期：1999-04-01 00:00:00

abstract：:A colloid gold strip (CGS) test for detecting antibodies to H5 subtype Avian Influenza Virus (AIV) was developed. The test is based on membrane chromatography and uses colloidal gold conjugated with inactivated AIV-H5N1 as the tracer. On the test strip, a baculovirus expressed haemagglutinin (HA) protein was used as t...

journal_title：Journal of virological methods

authors： Cui S,Chen C,Tong G

更新日期：2008-09-01 00:00:00

abstract：:The inactivation of hepatitis B virus (HBV) using two commercially available disinfectants was analysed. Indirect evidence of virus inactivation was obtained by examining the decrease in HBV-associated DNA polymerase and HBcAg activities after treatment with increasing concentrations of disinfectant. Inactivation was ...

journal_title：Journal of virological methods

authors： Howard CR,Dixon J,Young P,van Eerd P,Schellekens H

更新日期：1983-09-01 00:00:00

abstract：:A single-tube duplex and multiplex PCR was developed for the simultaneous detection of African cassava mosaic virus (ACMV), East African cassava mosaic Cameroon virus (EACMCV), East African cassava mosaic Malawi virus (EACMMV) and East African cassava mosaic Zanzibar virus (EACMZV), four cassava mosaic begomoviruses (...

journal_title：Journal of virological methods

authors： Aloyce RC,Tairo F,Sseruwagi P,Rey ME,Ndunguru J

更新日期：2013-04-01 00:00:00

abstract：:A high-throughput method of isolating and cloning geminivirus genomes from dried plant material, by combining an Extract-n-Amp-based DNA isolation technique with rolling circle amplification (RCA) of viral DNA, is presented. Using this method an attempt was made to isolate and clone full geminivirus genomes/genome com...

journal_title：Journal of virological methods

authors： Shepherd DN,Martin DP,Lefeuvre P,Monjane AL,Owor BE,Rybicki EP,Varsani A

更新日期：2008-04-01 00:00:00

abstract：:Viral susceptibility testing has been traditionally performed by the plaque reduction assay (PRA) which is laborious, time consuming, relatively expensive, and requires subjective input by the reader. An in situ cellular enzyme-linked immunosorbent assay (ELISA) has been developed with the potential to overcome many o...

journal_title：Journal of virological methods

authors： Myc A,Anderson MJ,Baker JR Jr

更新日期：1999-02-01 00:00:00

abstract：:Epstein-Barr virus (EBV) has been implicated in the pathogenesis of several human malignancies including B lymphomas and nasopharyngeal carcinoma. The EBV R transactivator (Rta) has been found to play essential roles in stimulating a lytic cycle and viral gene expression. Recently, it was shown that ELISA detecting se...

journal_title：Journal of virological methods

authors： Zhu YH,Wei YS,Li H,Liang WB,Du B,Zhang GQ,Zhang L

更新日期：2007-09-01 00:00:00

abstract：:A simple immunoperoxidase assay (IPA), adapted for detection of serum IgM antibodies to cytomegalovirus (CMV) is described. The antigen consisted of CMV infected human embryonic fibroblasts or isolated nuclei. The sera were absorbed with aggregated gamma-globulins prior to testing. Rabbit anti-human IgM peroxidase con...

journal_title：Journal of virological methods

authors： Singer Y,Kimmel N,Sarov I

更新日期：1985-05-01 00:00:00

abstract：:The Bacille Calmette Guerin (BCG), long valued for its role as a live vaccine for the prevention of tuberculosis, is being used as a recombinant delivery vehicle for foreign antigens, principally, for inducing long-lived specific humoral and cellular immunity. Hepatitis B and its sequelae are major public health probl...

journal_title：Journal of virological methods

authors： Rezende CA,De Moraes MT,De Souza Matos DC,McIntoch D,Armoa GR

更新日期：2005-04-01 00:00:00

abstract：:Two mosquito-borne flaviviruses, Murray Valley encephalitis (MVE) and Kunjin (KUN), are the aetiological agents of Australian encephalitis. MVE causes a severe and potentially fatal form of the disease while KUN is responsible for only a few relatively mild cases. Therefore it is important that serological tests used ...

journal_title：Journal of virological methods

authors： Hall RA,Broom AK,Hartnett AC,Howard MJ,Mackenzie JS

更新日期：1995-02-01 00:00:00

abstract：:Lactate dehydrogenase-elevating virus (LDV) can infect transplantable mouse tumors or xenograft tumors in mice through LDV-contaminated mouse biological materials, such as Matrigel, or through mice infected with LDV. LDV infects specifically mouse macrophages and alters immune system and tumor phenotype. The tradition...

journal_title：Journal of virological methods

authors： Liu H,Bockhorn J,Dalton R,Chang YF,Qian D,Zitzow LA,Clarke MF,Greene GL

更新日期：2011-05-01 00:00:00

abstract：:The Directigen Flu-A is an enzyme immunoassay for detecting in 15 min the influenza A nucleoproteinic antigen directly from specimens after passive adsorption on a cellulose membrane. The test was assessed using 160 frozen (-20 degrees C) specimens collected during the 1988-1989 A/H1N1 influenza epidemic and the 1989-...

journal_title：Journal of virological methods

authors： Chomel JJ,Remilleux MF,Marchand P,Aymard M

更新日期：1992-06-01 00:00:00

abstract：:A polymerase chain reaction (PCR) based on the use of multiple primers enabling the simultaneous detection of HPV-6b, -11, -16 and -18 in a single tube reaction was developed and validated on cervico-vaginal specimens, including tissues embedded in paraffin. This PCR setting proved to be specific and sensitive, allowi...

journal_title：Journal of virological methods

authors： Anceschi MM,Falcinelli C,Pieretti M,Cosmi EV

更新日期：1990-04-01 00:00:00

abstract：:The latent membrane protein 1 (LMP1) of Epstein-Barr virus (EBV) is one of two postulated viral oncogenic proteins. Sequence variations, and in particular a 30 base pair deletion variant called CAO, may define different disease populations. We developed a panel of rat monoclonal antibodies (MAb) specific for the non-w...

journal_title：Journal of virological methods

authors： Nicholls J,Hahn P,Kremmer E,Fröhlich T,Arnold GJ,Sham J,Kwong D,Grässer FA

更新日期：2004-03-01 00:00:00

abstract：:A new approach to simultaneous detection and typing of related agents by the multiplex polymerase chain reaction (PCR) is described. The reaction was been applied to human herpesviruses by nested amplification of fragments of the DNA polymerase genes. During the first amplification, primers were used as two equimolar ...

journal_title：Journal of virological methods

authors： Tenorio A,Echevarría JE,Casas I,Echevarría JM,Tabarés E

更新日期：1993-10-01 00:00:00

abstract：:A simple and rapid solid-phase reverse transcriptase assay was developed based on the use of poly(rA):oligo(dT)12-18 as template primer immobilized on DEAE cellulose paper squares to detect human immunodeficiency virus (HIV) and/or other retroviruses in cell culture supernatants. It was found that PEG (per se) -up to ...

journal_title：Journal of virological methods

authors： Somogyi PA,Gyuris A,Földes I

更新日期：1990-03-01 00:00:00

abstract：:The prevalence of porcine teschovirus (PTV) in swine herds in northeast China was investigated. In 2008-2009, 1384 samples of pig sera were collected from 42 farms in Shandong, Hebei, Heilongjiang, and Jilin provinces and in Tianjin City and were tested for specific antibody against PTV-8 by immunofluorescence assay. ...

journal_title：Journal of virological methods

authors： Qiu Z,Wang Z,Zhang B,Zhang J,Cui S

更新日期：2013-10-01 00:00:00