Abstract：
:Monoclonal antibodies (Mabs) against influenza B virus were obtained by immunizing mice with B/Nagasaki/1/87, one of the strains of the B/Victoria group. Immunoprecipitation analysis revealed that individual Mabs precipitated the nucleoprotein (NP), the matrix protein (M) or the hemagglutinin protein (HA). By using these Mabs by the peroxidase-antiperoxidase (PAP) staining method, a rapid detection and identification method for influenza B virus was established. Monolayers of Madin-Darby canine kidney cells in microplates were infected with each-strain and incubated for about 24 h, and then were subjected to the PAP staining method using the Mabs as the first antibody. Influenza B virus strains are classified into two major phylogenetic trees, the B/Victoria group and the B/Yamagata group. When anti-NP and anti-M antibodies were used in the PAP staining method, all 13 influenza B virus strains isolated from clinical specimens between 1940 and 1994 were detected regardless of the antigenic drift of the influenza virus. On the other hand, several anti-HA Mabs which reacted specifically with the strains of the B/Victoria group, did not react with any strain of the B/Yamagata group. In the 1996/97 influenza season in Osaka Prefecture in Japan, two antigenically distinct groups of influenza B virus strains were isolated. They belonged to different phylogenetic trees and were clearly distinguishable by the PAP staining method with anti-HA Mabs.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Nakagawa N,Maeda A,Kase T,Kubota R,Okuno Ydoi
10.1016/s0166-0934(99)00015-4subject
Has Abstractpub_date
1999-04-01 00:00:00pages
113-20issue
1eissn
0166-0934issn
1879-0984pii
S0166-0934(99)00015-4journal_volume
79pub_type
杂志文章abstract：:Rabbits and guinea pigs were immunized with purified bovine rotavirus. Immunoglobulin G fractions of the resulting antisera were used in a standard four-layer solid-phase enzyme immunoassay (EIA) for rotavirus antigen in human faecal specimens. Samples negative for rotavirus in electron microscopy, when diluted in sta...
journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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doi：10.1016/s0166-0934(98)00048-2
更新日期：1998-07-01 00:00:00
abstract：:Loop-mediated isothermal amplification is a novel method for rapid amplification of DNA. It has been adopted widely for the detection of virus because of its simplicity, rapidity, and specificity. A loop-mediated isothermal amplification assay was developed for the detection of porcine parvovirus. Four primers specifi...
journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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doi：10.1016/j.jviromet.2007.08.018
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journal_title：Journal of virological methods
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abstract：:Endothelial cell tropism is an inherent property of isolates of human cytomegalovirus (HCMV), and has been proposed as a pathogenicity factor of HCMV. Mutational approaches can be applied for analysis of the molecular determinants of endothelial cell tropism, but the evaluation of mutants is limited by the low reliabi...
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更新日期：2016-08-01 00:00:00
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journal_title：Journal of virological methods
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doi：10.1016/0166-0934(92)90121-s
更新日期：1992-09-01 00:00:00
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journal_title：Journal of virological methods
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更新日期：2012-02-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
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更新日期：2020-10-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/s0166-0934(98)00003-2
更新日期：1998-05-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/s0166-0934(97)00057-8
更新日期：1997-07-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
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更新日期：1987-10-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2013.01.026
更新日期：2013-04-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2019.05.009
更新日期：2019-08-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2021.114062
更新日期：2021-01-08 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/0166-0934(87)90056-5
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pub_type： 杂志文章
doi：10.1016/0166-0934(91)90108-c
更新日期：1991-10-01 00:00:00
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journal_title：Journal of virological methods
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更新日期：2009-04-01 00:00:00
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更新日期：2015-01-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
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更新日期：1991-06-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
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更新日期：1993-04-01 00:00:00
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doi：10.1016/s0166-0934(00)00194-4
更新日期：2000-08-01 00:00:00
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journal_title：Journal of virological methods
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doi：10.1016/j.jviromet.2014.06.023
更新日期：2014-10-01 00:00:00
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journal_title：Journal of virological methods
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更新日期：2013-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：1996-09-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
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更新日期：2005-09-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2011.12.009
更新日期：2012-03-01 00:00:00
abstract：:The open reading frame coding for the A-type inclusion body protein (ATI) of monkeypox virus (MPV) was identified and sequenced for two strains. Nucleotide sequence comparison revealed 72-95.3% homology with the reported open reading frame sequences of the ATIs of other orthopoxvirus species, such as variola, vaccinia...
journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/s0166-0934(98)00099-8
更新日期：1998-10-01 00:00:00
