Abstract:
:Loop-mediated isothermal amplification is a novel method for rapid amplification of DNA. It has been adopted widely for the detection of virus because of its simplicity, rapidity, and specificity. A loop-mediated isothermal amplification assay was developed for the detection of porcine parvovirus. Four primers specific for six regions of PPV non-structural protein 1 gene were designed with an online software. After amplifying at a constant temperature of 59-65 degrees C by Bst enzyme, a clear result was visible after 2.5% agarose gel electrophoresis. The sensitivity and specificity of this assay were evaluated by comparison with the polymerase chain reaction. The detection limit of the assay was shown to be equivalent to 5 PPV copies/reaction. Due to its specificity and simplicity, the assay should be a useful diagnostic tool for epidemiologic studies of PPV.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Chen CM,Cui SJdoi
10.1016/j.jviromet.2008.10.004subject
Has Abstractpub_date
2009-02-01 00:00:00pages
122-5issue
2eissn
0166-0934issn
1879-0984pii
S0166-0934(08)00372-8journal_volume
155pub_type
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