Detection of porcine parvovirus by loop-mediated isothermal amplification.

Abstract:

:Loop-mediated isothermal amplification is a novel method for rapid amplification of DNA. It has been adopted widely for the detection of virus because of its simplicity, rapidity, and specificity. A loop-mediated isothermal amplification assay was developed for the detection of porcine parvovirus. Four primers specific for six regions of PPV non-structural protein 1 gene were designed with an online software. After amplifying at a constant temperature of 59-65 degrees C by Bst enzyme, a clear result was visible after 2.5% agarose gel electrophoresis. The sensitivity and specificity of this assay were evaluated by comparison with the polymerase chain reaction. The detection limit of the assay was shown to be equivalent to 5 PPV copies/reaction. Due to its specificity and simplicity, the assay should be a useful diagnostic tool for epidemiologic studies of PPV.

journal_name

J Virol Methods

authors

Chen CM,Cui SJ

doi

10.1016/j.jviromet.2008.10.004

subject

Has Abstract

pub_date

2009-02-01 00:00:00

pages

122-5

issue

2

eissn

0166-0934

issn

1879-0984

pii

S0166-0934(08)00372-8

journal_volume

155

pub_type

杂志文章
  • Detection of bovine retrospumavirus by the polymerase chain reaction.

    abstract::A polymerase chain reaction (PCR) assay was developed for detection of bovine retrospumavirus (bovine syncytial virus; BSV) provirus DNA. Two different sets of oligonucleotide primers complementary to sequences located in the gag and the pol/env gene regions were used and compared for their ability to amplify the targ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/s0166-0934(98)00179-7

    authors: Pamba R,Jeronimo C,Archambault D

    更新日期:1999-03-01 00:00:00

  • Long amplicon sequencing for improved genetic characterization of African swine fever virus.

    abstract::African Swine Fever Virus (ASFV) causes a transmissible and fatal disease in pigs that is currently devastating global swine production. Efficient and economical collection of genetic data from ASFV field isolates is essential for bio-surveillance, to limit and control its spread, and to better understand ASF disease ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2020.113946

    authors: Meekins DA,Trujillo JD,Gaudreault NN,Morozov I,Pérez-Núñez D,Revilla Y,Richt JA

    更新日期:2020-11-01 00:00:00

  • Enhanced diagnostic efficiency of the polymerase chain reaction by co-amplification of multiple regions of HIV-1 and HIV-2.

    abstract::A method for co-amplification of multiple viral sequences of HIV-1 and HIV-2 by polymerase chain reaction was designed. The technique resulted in the specific detection of each type of virus and allowed the amplification of as few as two copies of target DNA. The amplification of multiple regions of the viral genome o...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/0166-0934(94)90053-1

    authors: Udaykumar,Heredia A,Soriano V,Bravo R,Epstein JS,Hewlett IK

    更新日期:1994-08-01 00:00:00

  • Quantitation of latency established by attenuated strains of Pseudorabies (Aujeszky's disease) virus.

    abstract::A quantitative and differential polymerase chain reaction (PCR) was developed that measures the ability of Pseudorabies virus (PRV) to colonize tissues that are targets for latency. This PCR is based on the co-amplification of viral target sequences and that of a gene of the host species: the porcine Nuclear Factor 1 ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/0166-0934(94)90183-x

    authors: Schang LM,Osorio FA

    更新日期:1994-12-01 00:00:00

  • A feline kidney cell line-based plaque assay for feline calicivirus, a surrogate for Norwalk virus.

    abstract::Feline calicivirus (FCV) has been used by researchers as a surrogate for Norwalk virus (NV), since they share a similar genomic organization, physicochemical characteristics, and are grouped in the same family, Caliciviridae. Unlike NV, however, FCV can grow in established cell lines and produce a syncytial form of cy...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/s0166-0934(02)00214-8

    authors: Bidawid S,Malik N,Adegbunrin O,Sattar SA,Farber JM

    更新日期:2003-02-01 00:00:00

  • Nonradioactive, photobiotin-labelled DNA probes for the routine diagnosis of barley yellow dwarf virus.

    abstract::Photobiotin was used to prepare biotinylated, nonradioactive nucleic acid probes for the detection of the RNA of barley yellow dwarf virus (BYDV) in plant extracts. A 1.7-kb cDNA of the PAV isolate of BYDV in the plasmid pUC8 vector was biotinylated and then used intact or as sonicated double-stranded DNA fragments. S...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/0166-0934(87)90007-3

    authors: Habili N,McInnes JL,Symons RH

    更新日期:1987-06-01 00:00:00

  • A simplified cloning strategy for the generation of an endothelial cell selective recombinant adenovirus vector.

    abstract::Specifically targeting adenoviral vectors to particular cell/tissue types can be achieved by genetically modifying the adenovirus fiber protein. Two common strategies are: (1) directly modifying the fiber gene in the adenovirus genome and (2) in trans supply of the modified fiber. The former however, suffers from diff...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2006.02.010

    authors: Mahanivong C,Krüger JA,Bian D,Reisfeld RA,Huang S

    更新日期:2006-07-01 00:00:00

  • Detection of novel swine origin influenza A virus (H1N1) by real-time nucleic acid sequence-based amplification.

    abstract::Rapid detection of novel swine origin influenza A virus (S-OIV) (H1N1) is crucial for timely implementation of infection control measures. In this study, a haemagglutinin (HA) gene-based real-time nucleic acid sequence-based amplification (NASBA) assay was developed for the specific detection of S-OIV (H1N1). The assa...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2009.10.025

    authors: Ge Y,Cui L,Qi X,Shan J,Shan Y,Qi Y,Wu B,Wang H,Shi Z

    更新日期:2010-02-01 00:00:00

  • Development of a blocking ELISA for detection of serum neutralizing antibodies against porcine circovirus type 2.

    abstract::A monoclonal antibody (Mab)-based blocking ELISA was developed for the detection of serum neutralizing antibodies to porcine circovirus type 2 (PCV2). The Mab with neutralizing activity, which was produced by immunizing a recombinant capsid protein of PCV2 expressed in insect cells, was used as the detector antibody. ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2010.09.023

    authors: Huang L,Lu Y,Wei Y,Guo L,Liu C

    更新日期:2011-01-01 00:00:00

  • Characterization and use of a recombinant retroviral system for the analysis of drug resistant HIV.

    abstract::A recombinant retroviral system was used for the analysis of early HIV breakthrough infection in the presence of antiviral drugs. The use of replication-defective HIV allowed a quantitative analysis of a single cycle of infection. This report characterizes this recombinant HIV system and demonstrates it's validity in ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/s0166-0934(97)00212-7

    authors: Medina DJ,Tung PP,Nelson CJ,Sathya B,Casareale D,Strair RK

    更新日期:1998-04-01 00:00:00

  • Immunogenicity and virulence of attenuated vaccinia virus Tian Tan encoding HIV-1 muti-epitope genes, p24 and cholera toxin B subunit in mice.

    abstract::No effective prophylactic or therapeutic vaccine against HIV-1 in humans is currently available. This study analyzes the immunogenicity and safety of a recombinant attenuated vaccinia virus. A chimeric gene of HIV-1 multi-epitope genes containing CpG ODN and cholera toxin B subunit (CTB) was inserted into Chinese vacc...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2015.03.007

    authors: Du S,Wang Y,Liu C,Wang M,Zhu Y,Tan P,Ren D,Li X,Tian M,Yin R,Li C,Jin N

    更新日期:2015-07-01 00:00:00

  • An international collaborative study to assess a set of reference reagents for HIV-1 PCR.

    abstract::An international collaborative study was performed to evaluate a set of PCR reference reagents for HIV diagnosis. Twenty-six laboratories from 9 countries analysed a proficiency panel of 10 coded DNA samples using the PCR reference reagents and protocols. For comparison, these coded samples were then assessed using a ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/0166-0934(92)90018-9

    authors: Bootman JS,Kitchin PA

    更新日期:1992-04-01 00:00:00

  • Expression of Coxsackievirus B4 proteins VP0 and 2C in Escherichia coli and generation of virus protein recognizing antisera.

    abstract::Coxsackievirus B4 (CBV-4) capsid protein VP0 and non-structural 2C protein were expressed and purified using a glutathione-S-transferase (GST) fusion protein expression system. We used a full-size CBV-4 cDNA as a template to amplify the genes by polymerase chain reaction (PCR). The genes were cloned into expression ve...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/s0166-0934(97)00150-x

    authors: Härkönen T,Hovi T,Roivainen M

    更新日期:1997-12-01 00:00:00

  • A novel and inexpensive application of RNAi technology to protect shrimp from viral disease.

    abstract::Large-scale production of long dsRNA is needed if antiviral applications of RNAi are to succeed in shrimp farm operations. A novel hairpin-RNA expression vector was developed based on the RNA-dependent RNA polymerase (RdRp) gene of yellow head virus (YHV), the cause of a lethal shrimp disease. Using transformed RNase-...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2009.08.010

    authors: Saksmerprome V,Charoonnart P,Gangnonngiw W,Withyachumnarnkul B

    更新日期:2009-12-01 00:00:00

  • Development of a capsid based competitive inhibition enzyme-linked immunosorbent assay for detection of bovine immunodeficiency virus antibodies in cattle and buffalo serum.

    abstract::The aim of this study was to develop a more specific and sensitive competitive inhibition ELISA (CI-ELISA) than the currently used indirect ELISA for detection of antibodies to bovine immunodeficiency virus (BIV) in cattle and buffaloes. Murine monoclonal antibodies (MAbs) were generated against a recombinant capsid (...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2007.11.008

    authors: Bhatia S,Sood R,Bhatia AK,Pattnaik B,Pradhan HK

    更新日期:2008-03-01 00:00:00

  • Development of chemiluminescent probe hybridization, RT-PCR and nucleic acid cycle sequencing assays of Sabin type 3 isolates to identify base pair 472 Sabin type 3 mutants associated with vaccine associated paralytic poliomyelitis.

    abstract::Sabin type 3 polio vaccine virus is the most common cause of poliovaccine associated paralytic poliomyelitis. Vaccine associated paralytic poliomyelitis cases have been associated with Sabin type 3 revertants containing a single U to C substitution at bp 472 of Sabin type 3. A rapid method of identification of Sabin t...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/s0166-0934(97)00114-6

    authors: Old MO,Logan LH,Maldonado YA

    更新日期:1997-11-01 00:00:00

  • Rapid acquisition of entire DNA polymerase gene of a novel herpesvirus from green turtle fibropapilloma by a genomic walking technique.

    abstract::A 4837-bp sequence of a newfound green turtle herpesvirus (GTHV), implicated in the etiology of green turtle fibropapilloma, was obtained from tumor tissues of a green turtle with fibropapilloma using a genomic walking method based on restriction enzyme digestion, self-ligation and inverse polymerase chain reaction (I...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/s0166-0934(00)00267-6

    authors: Yu Q,Hu N,Lu Y,Nerurkar VR,Yanagihara R

    更新日期:2001-02-01 00:00:00

  • Multiplication of Taura syndrome virus in primary hemocyte culture of shrimp (Penaeus vannamei).

    abstract::The propagation of Taura syndrome virus (TSV) in primary hemocyte culture of Pacific white shrimp (Penaeus vannamei) was investigated. Purified TSV was inoculated into a 24 h old primary hemocyte culture and the development of cytopathic effects was monitored. The cell morphology started changing within 6 h post-inocu...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2010.12.020

    authors: George SK,Kaizer KN,Betz YM,Dhar AK

    更新日期:2011-03-01 00:00:00

  • A quantitative duplex PCR technique for measuring amounts of cell-associated Marek's disease virus: differences in two populations of lymphoma cells.

    abstract::A duplex polymerase chain reaction (PCR) was developed to measure Marek's disease virus (MDV) load in two subpopulations of Marek's disease (MD) lymphoma cells from chickens. PCR primers were designed using the sequence of the MDV-ICP4 gene and the chicken IFNgamma gene. Each set of primers was present in the same rea...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/s0166-0934(99)00075-0

    authors: Burgess SC,Davison TF

    更新日期:1999-09-01 00:00:00

  • Chemical inhibition of foamyvirus in primary baboon (Papio cynocephalus) kidney cells.

    abstract::This report describes the conditions for the use of aluminum chloride (AlCl3) in growth and maintenance media for the suppression or inhibition of simian foamyviruses (SFV) in primary baboon kidney (BAK) and rabbit kidney (RK) cell cultures. When RK cells were planted in medium containing AlCl3, infected with SFV, and...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/0166-0934(82)90066-0

    authors: Weiss SA,Heberling RL,Kalter SS

    更新日期:1982-05-01 00:00:00

  • Differential expression of two isolates of beak and feather disease virus capsid protein in Escherichia coli.

    abstract::Expression of recombinant beak and feather disease virus (BFDV) capsid-associated protein (Cap) has relied on inefficient techniques that typically produce low yields or use specialized expression systems, which greatly increase the cost and expertise required for mass production. An Escherichia coli system was used t...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2013.01.020

    authors: Patterson EI,Swarbrick CM,Roman N,Forwood JK,Raidal SR

    更新日期:2013-04-01 00:00:00

  • PG-4 cell plaque assay for xenotropic murine leukemia virus.

    abstract::Xenotropic murine leukemia virus (X-MuLV) is often used in retrovirus elimination studies involving rodent cells. Currently, X-MuLV is measured using a focus-forming assay on mink (MiCl1 S+L-) or cat (PG-4 S+L-) cell lines. An easier and quicker PG-4 cell plaque assay, which retains the statistical reproducibility of ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/s0166-0934(99)00064-6

    authors: Li Z,Blair M,Thorner L

    更新日期:1999-08-01 00:00:00

  • Generation and molecular docking analysis of specific single-chain variable fragments selected by phage display against the recombinant nucleocapsid protein of fig mosaic virus.

    abstract::The mosaic disease caused by fig mosaic virus (FMV) is considered the plague of fig worldwide. A naïve phage display library, raised against the recombinant nucleocapsid protein of FMV (FMV-Np) was screened to obtain specific monoclonal recombinant antibodies in the form of single chain variable fragments (scFvs). Aft...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2019.113796

    authors: Shahmirzaie M,Safarnejad MR,Rakhshandehroo F,Safarpour H,Shirazi FH,Zamanizadeh HR,Elbeaino T

    更新日期:2020-02-01 00:00:00

  • Isolation of a cell line for rapid and sensitive histochemical assay for the detection of herpes simplex virus.

    abstract::A cell line which can be used in a simple, sensitive, and rapid histochemical assay was isolated for detection of herpes simplex virus (HSV). The cell line was derived by selection of G418 resistant colonies following co-transfection of baby hamster kidney cells with a plasmid which contains a G418 antibiotic resistan...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/0166-0934(92)90110-y

    authors: Stabell EC,Olivo PD

    更新日期:1992-08-01 00:00:00

  • A multiplexed TaqMan assay for the detection of arthropod-borne flaviviruses.

    abstract::Arboviruses in the genus Flavivirus are major causes of human disease worldwide, and yet they are widely under reported. This is partially due to the classical methods of detection, using virologic or immunologic techniques, which typically lack sensitivity and specificity or require biological containment facilities ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2007.05.001

    authors: Dyer J,Chisenhall DM,Mores CN

    更新日期:2007-10-01 00:00:00

  • Evaluation of a prototype HCV NS5b assay for typing strains of hepatitis C virus isolated from Tunisian haemodialysis patients.

    abstract::Hepatitis C virus (HCV) strains isolated from 68 haemodialysis Tunisian patients exhibiting chronic infection were genotyped targeting the NS5b region of the HCV genome using a prototype assay developed by Bayer HealthCare-Diagnostics (TRUGENE NS5b HCV). The overall results were compared to those obtained with another...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2004.03.002

    authors: Othman SB,Trabelsi A,Monnet A,Bouzgarrou N,Grattard F,Beyou A,Bourlet T,Pozzetto B

    更新日期:2004-08-01 00:00:00

  • Chicken single-chain variable fragments against the SARS-CoV spike protein.

    abstract::The major concern for severe acute respiratory syndrome (SARS), caused by the SARS-associated coronavirus (SARS-CoV), is the lack of diagnostic and therapeutic agents. Using a phage display technology in a chicken system, high-affinity monoclonal antibody fragments against the SARS-CoV spike protein were characterized...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2007.06.010

    authors: Lee YC,Leu SJ,Hu CJ,Shih NY,Huang IJ,Wu HH,Hsieh WS,Chiang BL,Chiu WT,Yang YY

    更新日期:2007-12-01 00:00:00

  • Use of hepatitis E IgG avidity for diagnosis of hepatitis E infection.

    abstract::The diagnosis of acute hepatitis E infection is based on the detection of HEV RNA or specific IgM in immunocompetent patients. Viraemia and excretion of HEV RNA in faeces are not observed in all patients and commercial kits vary in their performance for anti-HEV IgM detection. Additional diagnostic tests must therefor...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2009.11.028

    authors: Bigaillon C,Tessé S,Lagathu G,Nicand E

    更新日期:2010-03-01 00:00:00

  • Proteasome inhibition reduces avian reovirus replication and apoptosis induction in cultured cells.

    abstract::The interplay between avian reovirus (ARV) replication and apoptosis and proteasome pathway was studied in cultured cells. It is shown that inhibition of the proteasome did not affect viral entry and host cell translation but had influence on ARV replication and ARV-induced apoptosis. Evidence is provided to demonstra...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2008.03.016

    authors: Chen YT,Lin CH,Ji WT,Li SK,Liu HJ

    更新日期:2008-07-01 00:00:00

  • Detection of feline Coronavirus in effusions of cats with and without feline infectious peritonitis using loop-mediated isothermal amplification.

    abstract::Feline infectious peritonitis (FIP) is a fatal disease in cats worldwide. The aim of this study was to test two commercially available reaction mixtures in a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay to detect feline Coronavirus (FCoV) in body cavity effusions of cats with and withou...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2018.03.003

    authors: Günther S,Felten S,Wess G,Hartmann K,Weber K

    更新日期:2018-06-01 00:00:00