Abstract：
:The species cowpox virus (CPXV), genus Orthopoxvirus (OPV), consists of isolates highly variable in their biological properties and their genotypes. A TaqMan PCR assay for the specific detection of CPXV DNA based on sequences of the ORF D11L has been developed recently. (Gavrilova et al., 2010; Shchelkunov et al., 2011); however, a rather limited panel of CPXV stains has been used. When a much larger panel of 47 CPXV DNAs has been tested, three strains could not be amplified at all because of large deletions in their respective ORF D11L. In addition, a deletion of 23bp led to low-efficiency detection of five other CPXV strains. To solve this problem a new primer/probe combinations was selected based on sequences of ORF D8L, and a new real-time PCR method for (i) a genus-specific detection of OPVs and (ii) a simultaneous CPXV-specific differentiation is described in this study. The specificity and sensitivity were assessed by analyzing DNA of 67 strains belonging to human-pathogenic OPV species, including variola virus, as well as specimens of CPXV-infected mice.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Maksyutov RA,Gavrilova EV,Meyer H,Shchelkunov SNdoi
10.1016/j.jviromet.2014.10.004subject
Has Abstractpub_date
2015-01-01 00:00:00pages
8-11eissn
0166-0934issn
1879-0984pii
S0166-0934(14)00394-2journal_volume
211pub_type
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