Development of a capsid based competitive inhibition enzyme-linked immunosorbent assay for detection of bovine immunodeficiency virus antibodies in cattle and buffalo serum.


:The aim of this study was to develop a more specific and sensitive competitive inhibition ELISA (CI-ELISA) than the currently used indirect ELISA for detection of antibodies to bovine immunodeficiency virus (BIV) in cattle and buffaloes. Murine monoclonal antibodies (MAbs) were generated against a recombinant capsid (CA) protein of bovine immunodeficiency virus. Of the 13 anti-CA MAbs developed, MAb-9G10 was selected for CI-ELISA based on the maximum inhibition (98%) obtained with reference BIV antibody positive serum. Based on the distribution of percent inhibition of known negative sera (n=50), a cut-off value was set at 40% inhibition. The MAb-based CI-ELISA showed much higher agreement (concordance: 95.4%) than the indirect ELISA (concordance: 77.8%) with Western blot. Out of 672 sera of cattle and buffaloes tested by CI-ELISA from four states of India, 22% (113/516) of cattle and 19% (30/156) of buffalo were sero-positive for BIV with an overall seroprevalence of 21% (143/672) in India.


J Virol Methods


Bhatia S,Sood R,Bhatia AK,Pattnaik B,Pradhan HK




Has Abstract


2008-03-01 00:00:00














  • Infectivity assays for the Autographa californica nuclear polyhedrosis virus using Spodoptera littoralis cells.

    abstract::Tissue culture ID50 and plaque assays for the Autographa californica nuclear polyhedrosis virus, using the Spodoptera littoralis cell line HPB-SL, were developed. Direct comparison using these assay methods showed that these cells were as susceptible to infection as the more commonly used Spodoptera frugiperda cell li...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Roberts PL

    更新日期:1985-01-01 00:00:00

  • Development of universal primers for detection of potato carlaviruses by RT-PCR.

    abstract::To facilitate efficient and accurate detection of potato-infecting carlaviruses, degenerated universal primers were designed based on conserved amino acid and nucleotide sequences. Two sense primers, Car-F1 and Car-F2, were based on the amino acid sequences "SNNMA" and "GLGVPTE", respectively, in the coat protein. The...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Nie X,Bai Y,Molen TA,Desjardins DC

    更新日期:2008-05-01 00:00:00

  • Kinetics of rubella-specific IgM antibody response in postnatal rubella infection.

    abstract::The serological diagnosis of primary postnatal rubella infection is based on detection of rubella-virus-specific IgM antibody or a four-fold rise in rubella-specific IgG antibody. Although there are several different methods of enzyme immunoassays that are commercially available, the cost benefit evaluation makes them...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Cordoba P,Nates S,Mahony J,Zapata M

    更新日期:1991-09-01 00:00:00

  • A rapid microneutralization assay for the measurement of neutralizing antibody reactive with human cytomegalovirus.

    abstract::We have developed a murine monoclonal antibody reactive with a major immediate early 72,000 dalton protein of human cytomegalovirus and utilized this reagent in a rapid virus titration and microneutralization assay. Because of the early expression of this virus encoded protein, both assays could be accomplished within...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Andreoni M,Faircloth M,Vugler L,Britt WJ

    更新日期:1989-02-01 00:00:00

  • The impact of PCR-generated recombination on diversity estimation of mixed viral populations by deep sequencing.

    abstract::Ultra-deep pyrosequencing (UDPS) of targeted amplicons allows to determine a large number of individual sequence reads from a single PCR product, and this approach is thus extremely valuable for analysis of mixed viral populations. A mixture of genetically distinct DNA templates, however, may lead to the generation of...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Görzer I,Guelly C,Trajanoski S,Puchhammer-Stöckl E

    更新日期:2010-10-01 00:00:00

  • Coupling of foot-and-mouth disease virus to sheep red blood cells using tannic acid for immunological assays.

    abstract::A technique for coupling foot-and-mouth disease virus (FMDV) to tanned sheep red blood cells (SRBC) is reported. Different parameters influencing the procedure were studied. Subtypes C2, C3, O1 and A24 were used as antigens, and guinea pig hyperimmune sera obtained were tested for specific antibody in passive hemagglu...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Pujol CA,Feledi CA,Massouh EJ

    更新日期:1988-08-01 00:00:00

  • Enzymatic amplification of latent pseudorabies virus nucleic acid sequences.

    abstract::To investigate various aspects of the latency of pseudorabies virus in swine (PRV, suid herpesvirus 1) we developed in vitro nucleic acid amplification methods based upon the polymerase chain reaction. Primers flanking a 156-bp region of the pseudorabies virus gp II gene were annealed to purified PRV DNA as well as DN...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Lokensgard JR,Thawley DG,Molitor TW

    更新日期:1991-09-01 00:00:00

  • Cloning and expression of two fragments of the S gene of canine coronavirus type I.

    abstract::Two fragments, S66 and S55, of the S glycoprotein of the newly identified canine coronavirus type I (CCoV type I), were expressed in a procariotic system. The purified recombinant proteins of 350 and 366 amino acids in length, respectively, were employed to develop an enzyme-linked immunosorbent assay (ELISA) for the ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Pratelli A,Elia G,Decaro N,Tola S,Tinelli A,Martella V,Rocca S,Tempesta M,Buonavoglia C

    更新日期:2004-04-01 00:00:00

  • Construction of infectious cDNA clone derived from a classical swine fever virus field isolate in BAC vector using in vitro overlap extension PCR and recombination.

    abstract::To develop reverse genetics system of RNA viruses, cloning of full-length viral genome is required which is often challenging due to many steps involved. In this study, we report cloning of full-length cDNA from an Indian field isolate (CSFV/IVRI/VB-131) of classical swine fever virus (CSFV) using in vitro overlap ext...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Kamboj A,Saini M,Rajan LS,Patel CL,Chaturvedi VK,Gupta PK

    更新日期:2015-12-15 00:00:00

  • Rapid detection of avian influenza virus H5N1 in chicken tracheal samples using an impedance aptasensor with gold nanoparticles for signal amplification.

    abstract::Highly pathogenic avian influenza virus H5N1 is a continuous threat to public health and poultry industry. The recurrence of the H5N1 led us to develop a robust, specific, and rapid detection method for the virus. In this study, an impedance aptasensor was developed for the virus detection using specific H5N1 aptamer ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Karash S,Wang R,Kelso L,Lu H,Huang TJ,Li Y

    更新日期:2016-10-01 00:00:00

  • Primary diagnosis of foot-and-mouth disease by reverse transcription polymerase chain reaction.

    abstract::Universal and serotype-specific primer sets were used in simple reverse transcription polymerase chain reaction (RT-PCR) assays on field samples of epithelium and vesicular fluid to determine their suitability for primary diagnosis of all seven serotypes of foot-and-mouth disease (FMD). The specificity of reactions wa...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Reid SM,Ferris NP,Hutchings GH,Samuel AR,Knowles NJ

    更新日期:2000-09-01 00:00:00

  • Reactivity and amplification efficiency of the NASBA HIV-1 RNA amplification system with regard to different HIV-1 subtypes.

    abstract::In view of the genetic diversity of the human immunodeficiency virus Type 1, we assessed the sensitivity and quantification efficiency of the HIV-1 RNA NASBA amplification system with respect to different HIV-1 subtypes and recombinants. Twenty cell culture supernatants representing 17 HIV-1 group M and 3 group O stra...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Gobbers E,Fransen K,Oosterlaken T,Janssens W,Heyndrickx L,Ivens T,Vereecken K,Schoones R,van de Wiel P,van der Groen G

    更新日期:1997-07-01 00:00:00

  • A preliminary evaluation of five HIV antigen detection assays.

    abstract::Five commercial assays for detecting HIV antigen were evaluated using a panel of 40 coded samples in six laboratories. All assays were capable of detecting HIV-1 antigen (HIV-1 Ag), and are likely to prove useful for monitoring supernatant fluids from a variety of cell cultures. The concentration of HIV-1 protein whic...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Healey DS,Maskill WJ,Neate EV,Beaton F,Gust ID

    更新日期:1988-06-01 00:00:00

  • Improved detection of simian immunodeficiency virus RNA by in situ hybridization in fixed tissue sections: combined effects of temperatures for tissue fixation and probe hybridization.

    abstract::In situ hybridization detection of viral RNAs in formaldehyde-fixed tissue specimens is used frequently to characterize the extent of viral replication within host tissues. The ability to determine the level of expression of viral RNAs in situ is dependent upon many factors including the extent of cross-linking during...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Fallert BA,Reinhart TA

    更新日期:2002-01-01 00:00:00

  • A comparison between one first generation and three second generation anti-HCV ELISAs: an investigation in high- and low-risk subjects in correlation with recombinant immunoblot assay and polymerase chain reaction.

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    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Bååth L,Widell A,Nordenfelt E

    更新日期:1992-12-01 00:00:00

  • A latex agglutination assay to quantify the amount of hemagglutinin protein in adjuvanted low-dose influenza monovalent vaccines.

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    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Buffin S,Ikhelef N,Prudent J,Dubayle J,Nougarede N,Varenne MP,Moste C,Legastelois I

    更新日期:2018-01-01 00:00:00

  • Towards construction of viral vectors based on avian coronavirus infectious bronchitis virus for gene delivery and vaccine development.

    abstract::Manipulation of the coronavirus genome to accommodate and express foreign genes is an attractive approach for gene delivery and vaccine development. By using an infectious cloning system developed recently for the avian coronavirus infectious bronchitis virus (IBV), the enhanced green fluorescent protein (EGFP) gene, ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Shen H,Fang SG,Chen B,Chen G,Tay FP,Liu DX

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    abstract::Recombinant Nef-protein of HIV-1 Bru derived from Escherichia coli revealed heparin-binding activity. This property was used to purify the Nef-protein by a one-step procedure, yielding about 90% homogenous Nef-protein as evaluated by silver staining. The Nef-protein was soluble without denaturing agents. Native foldin...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Kohleisen B,Gaedigk-Nitschko K,Ohlmann M,Götz E,Ostolaza H,Goni FM,Erfle V

    更新日期:1996-06-01 00:00:00

  • Infection and replication of a planthopper transmitted virus-rice stripe virus in rice protoplasts.

    abstract::Rice stripe virus (RSV), a planthopper-transmitted virus, was inoculated into rice protoplasts, and a one-step growth curve was determined. The amount of virus in the protoplasts decreased following the inoculation, and then increased after 8 h. The replication of RSV reached its peak 20 h after inoculation. RSV repli...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Yang W,Wang X,Wang S,Yie Y,Tien P

    更新日期:1996-05-01 00:00:00

  • A simplified cloning strategy for the generation of an endothelial cell selective recombinant adenovirus vector.

    abstract::Specifically targeting adenoviral vectors to particular cell/tissue types can be achieved by genetically modifying the adenovirus fiber protein. Two common strategies are: (1) directly modifying the fiber gene in the adenovirus genome and (2) in trans supply of the modified fiber. The former however, suffers from diff...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Mahanivong C,Krüger JA,Bian D,Reisfeld RA,Huang S

    更新日期:2006-07-01 00:00:00

  • Detection of episomal banana streak badnavirus by IC-PCR.

    abstract::A polymerase chain reaction (PCR) based strategy to detect episomal banana streak badnavirus (BSV) in banana and plantain plants that carry integrated BSV sequences was developed. Antisera used in immuno-capture polymerase chain reaction (IC-PCR) are capable of binding a large number of BSV serotypes. The primers used...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Harper G,Dahal G,Thottappilly G,Hull R

    更新日期:1999-04-01 00:00:00

  • Detection of cytomegalovirus by 24-well plate centrifugation assay using a monoclonal antibody to an early nuclear antigen and by conventional cell culture.

    abstract::During a 12-month period, two methods for detection of cytomegalovirus (CMV) in 1624 clinical specimens were compared: (1) centrifugal inoculation of MRC-5 cells on coverslips in 24-well plates and staining with a monoclonal antibody to CMV early nuclear antigen after incubation for 40 h (EA assay), and (2) convention...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Woods GL,Young A,Johnson A,Thiele GM

    更新日期:1987-12-01 00:00:00

  • Simple microwave and thermal cycler boiling methods for preparation of cervicovaginal lavage cell samples prior to PCR for human papillomavirus detection.

    abstract::Sample preparation is an important step in the detection of viral DNA by the polymerase chain reaction (PCR) technique. The method used should achieve release of cellular DNA with the minimum of manipulation steps so as to reduce the possibility of contamination. The present report demonstrates that either microwaving...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Lou YK,Qin H,Molodysky E,Morris BJ

    更新日期:1993-09-01 00:00:00

  • Development of a Plexor real-time PCR assay for the detection of porcine circovirus type 2.

    abstract::A novel, real-time PCR system for the detection of porcine circovirus type 2 (PCV2) was developed. The system employed Plexor technology and detected 10(8)-10(1) copies per reaction of PCV2 DNA within a recombinant plasmid. The examination of clinical material showed consistent diagnostic sensitivity when samples cont...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Vlasakova M,Jackova A,Leskova V,Vilcek S

    更新日期:2012-02-01 00:00:00

  • A combined genotypic and phenotypic human immunodeficiency virus type 1 recombinant virus assay for the reverse transcriptase and integrase genes.

    abstract::With the approval of the first HIV-1 integrase inhibitor raltegravir and a second one in phase III clinical development (elvitegravir), genotypic and phenotypic resistance assays are required to guide antiretroviral therapy and to investigate treatment failure. In this study, a genotypic and phenotypic recombinant vir...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Van Baelen K,Rondelez E,Van Eygen V,Ariën K,Clynhens M,Van den Zegel P,Winters B,Stuyver LJ

    更新日期:2009-11-01 00:00:00

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    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Murtaugh MP,Lin GF,Haggard DL,Weber AF,Meiske JC

    更新日期:1991-06-01 00:00:00

  • A PCR based DNA hybridisation capture system for the detection of human cytomegalovirus. A comparative study with other identification methods.

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    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Mansy F,Brancart F,Liesnard C,Bollen A,Godfroid E

    更新日期:1999-07-01 00:00:00

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    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Faccini S,De Mattia A,Chiapponi C,Barbieri I,Boniotti MB,Rosignoli C,Franzini G,Moreno A,Foni E,Nigrelli AD

    更新日期:2017-05-01 00:00:00

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    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Zhou D,Fan X,Tan D,Xu Y,Tavis JE,Di Bisceglie AM

    更新日期:2007-05-01 00:00:00

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    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Patterson EI,Swarbrick CM,Roman N,Forwood JK,Raidal SR

    更新日期:2013-04-01 00:00:00