Abstract：
:The aim of this study was to develop a more specific and sensitive competitive inhibition ELISA (CI-ELISA) than the currently used indirect ELISA for detection of antibodies to bovine immunodeficiency virus (BIV) in cattle and buffaloes. Murine monoclonal antibodies (MAbs) were generated against a recombinant capsid (CA) protein of bovine immunodeficiency virus. Of the 13 anti-CA MAbs developed, MAb-9G10 was selected for CI-ELISA based on the maximum inhibition (98%) obtained with reference BIV antibody positive serum. Based on the distribution of percent inhibition of known negative sera (n=50), a cut-off value was set at 40% inhibition. The MAb-based CI-ELISA showed much higher agreement (concordance: 95.4%) than the indirect ELISA (concordance: 77.8%) with Western blot. Out of 672 sera of cattle and buffaloes tested by CI-ELISA from four states of India, 22% (113/516) of cattle and 19% (30/156) of buffalo were sero-positive for BIV with an overall seroprevalence of 21% (143/672) in India.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Bhatia S,Sood R,Bhatia AK,Pattnaik B,Pradhan HKdoi
10.1016/j.jviromet.2007.11.008subject
Has Abstractpub_date
2008-03-01 00:00:00pages
218-25issue
1-2eissn
0166-0934issn
1879-0984pii
S0166-0934(07)00445-4journal_volume
148pub_type
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