Abstract：
:28 isolates of canine parvovirus type-2 (CPV-2) were obtained from dogs with hemorrhagic gastroenteritis in Italy. The antigenic structure of CPV-2 isolates was characterized, using four discriminating monoclonal antibodies. In addition, four vaccinal strains were examined. Similar to reports from Australia and the United Kingdom, a much higher prevalence of CPV-2a (25/28 isolates) was observed than the other variant type, CPV-2b (3/28 isolates). DNA fragments (2.2 kbp) of representative strains of CPV-2, CPV-2a and CPV-2b were amplified by the polymerase chain reaction (PCR) and the products were digested by the restriction enzymes (RE) RsaI, HpaII, HindIII and PvuII. The RvaI enzyme allows the differentiation of CPV-2 from CPV-2a and CPV-2b.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Sagazio P,Tempesta M,Buonavoglia D,Cirone F,Buonavoglia Cdoi
10.1016/s0166-0934(98)00055-xsubject
Has Abstractpub_date
1998-08-01 00:00:00pages
197-200issue
2eissn
0166-0934issn
1879-0984pii
S016609349800055Xjournal_volume
73pub_type
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