Safety factors involved in the extraction of biologically active proteins from human immunodeficiency virus.

abstract：:A detailed growth and purification scheme suitable for producing relatively large quantities of fully active, pure SV40 is presented together with data on recovery and purity at each step of the procedure. The scheme was designed to prevent the initial binding of virus to cell components as well as contamination of th...

journal_title：Journal of virological methods

authors： Rosenberg BH,Deutsch JF,Ungers GE

更新日期：1981-10-01 00:00:00

abstract：:We developed a non-radioisotopic (non-RI) reverse transcriptase assay (RTA). The reverse transcriptase (RT) incorporates biotin-11-deoxyuridine-triphosphate (bio-dUTP) using a poly(rA) template hybridized with oligo(dT) primer that is immobilized on the surface of a 96-well microtiter plate. This assay is thus semi-au...

journal_title：Journal of virological methods

authors： Urabe T,Sano K,Tanno M,Mizoguchi J,Otani M,Lee MH,Takasaki T,Kusakabe H,Imagawa DT,Nakai M

更新日期：1992-11-01 00:00:00

abstract：:The humoral immune response of sheep infected with bluetongue virus serotypes 3, 9 and 16 was monitored by plaque inhibition (PI), blocking ELISA (BELISA) and indirect ELISA over a period of 63 days post-infection. Results indicated that testing of a single plasma or serum sample by both a BELISA and an indirect ELISA...

journal_title：Journal of virological methods

authors： Lunt RA,Blacksell SD,Newberry KM

更新日期：1994-06-01 00:00:00

abstract：:Herpes simplex virus type 1 (HSV-1) UL3 protein is a nuclear protein. In this study, the molecular mechanism of the subcellular localization of UL3 was characterized by fluorescence microscopy in living cells. A nuclear localization signal (NLS) and a nuclear export signal (NES) were also identified. UL3 was demonstra...

journal_title：Journal of virological methods

authors： Zheng C,Lin F,Wang S,Xing J

更新日期：2011-11-01 00:00:00

abstract：:Receptor-mediated endocytosis was employed for a highly efficient transport of oligodeoxynucleotides into hepatoma cell line PLC/PRF/5. The oligodeoxynucleotides were bound to the asialofetuin-poly-L-lysine conjugate and this complex was internalized by the cells via asialoglycoprotein receptor, an endocytic receptor ...

journal_title：Journal of virological methods

authors： Reinis M,Damková M,Korec E

更新日期：1993-04-01 00:00:00

abstract：:The sensitivity and specificity of four modes of two assays, immediate early nuclear antigen detection in cell culture (IENAD) at 24 and 48 h post-infection (p.i.) by immunofluorescence using a murine monoclonal antibody, and dot-blot DNA hybridisation with overnight or prolonged autoradiography using the 32P-labelled...

journal_title：Journal of virological methods

authors： Morris DJ,Lomax J,Fox AJ,Corbitt G

更新日期：1987-10-01 00:00:00

abstract：:Using a multiplexed LNA-based Taqman assay, RT-digital PCR (RT-dPCR) was performed in a prefabricated microfluidic device that monitored absolute viral load in native and immortalized cell lines, overall precision of detection, and the absolute detection limit of an occult RNA virus GB Virus Type C (GBV-C). RT-dPCR ha...

journal_title：Journal of virological methods

authors： White RA 3rd,Quake SR,Curr K

更新日期：2012-01-01 00:00:00

abstract：:A protocol suitable for the detection of rabies virus and the related European bat lyssaviruses type 1 and 2 is described. In situ hybridisation, employing digoxigenin labelled riboprobes was used for the detection of lyssavirus RNA in mouse-infected brain tissue. The principal advantage of this technique, compared to...

journal_title：Journal of virological methods

authors： Finnegan CJ,Brookes SM,Johnson L,Fooks AR

更新日期：2004-11-01 00:00:00

abstract：:Coliphages are alternative fecal indicators that may be suitable surrogates for viral pathogens, but majority of standard detection methods utilize insufficient volumes for routine detection in environmental waters. We compared three somatic and F+ coliphage methods based on a paired measurement from 1 L samples colle...

journal_title：Journal of virological methods

authors： McMinn BR,Rhodes ER,Huff EM,Wanjugi P,Ware MM,Nappier SP,Cyterski M,Shanks OC,Oshima K,Korajkic A

更新日期：2018-11-01 00:00:00

abstract：:A recombinant hepatitis B surface antigen (HBsAg) has been produced in the yeast Saccharomyces cerevisiae and used as a vaccine against hepatitis B virus (HBV) infection. The present study aimed to optimize the extraction of recombinant virus-like particles (rVLPs) to develop a simple purification procedure based on g...

journal_title：Journal of virological methods

authors： Hadiji-Abbes N,Martin M,Benzina W,Karray-Hakim H,Gergely C,Gargouri A,Mokdad-Gargouri R

更新日期：2013-01-01 00:00:00

abstract：:Human immunodeficiency virus (HIV) encodes a transcription trans-activator (Tat) with an essential role in the transcriptional elongation of viral RNA based on the viral promoter long terminal repeat (LTR). Tat-mediated transcription is conserved and can be distinguished from host transcription, so it is a therapeutic...

journal_title：Journal of virological methods

authors： Shin Y,Choi BS,Kim KC,Kang C,Kim K,Yoon CH

更新日期：2017-11-01 00:00:00

abstract：:In this study, we aim to determine what effects prolonged storage and repeated freeze/thaw cycles have on the stability of influenza A(H1N1)pdm09 (influenza A/H1N1)RNA. Cloned influenza A/H1N1 RNA transcripts were serially diluted from 8.0-1.0 log10 copies/μl. RT-qPCR was used to measure RNA loss in transcripts stored...

journal_title：Journal of virological methods

authors： Granados A,Petrich A,McGeer A,Gubbay JB

更新日期：2017-09-01 00:00:00

abstract：:Accurate detection and quantitation of viruses can be beneficial to plant-virus interaction studies. In this study, three SYBR green real-time RT-PCR assays were developed to quantitate grapevine leafroll-associated virus 3 (GLRaV-3) in infected vines. Three genomic regions (ORF1a, coat protein and 3'UTR) were targete...

journal_title：Journal of virological methods

authors： Bester R,Pepler PT,Burger JT,Maree HJ

更新日期：2014-12-15 00:00:00

abstract：:One of the most important steps when preparing a live attenuated vaccine is the assessment of the level of attenuation in target animals. It is costly and time consuming as it requires, on each occasion, a large number of susceptible animals and contained accommodation. This study assessed the consistency of the bovin...

journal_title：Journal of virological methods

authors： Franchi P,Mercante MT,Ronchi GF,Armillotta G,Ulisse S,Molini U,Di Ventura M,Lelli R,Savini G,Pini A

更新日期：2008-11-01 00:00:00

abstract：:An inducible in vitro cell culture system was developed to assay HCV replication by direct biochemical means. A transcription plasmid containing a T7 promoter at the 5' end, full-length cDNA of the HCV genome, a ribozyme sequence from the antigenomic strand of hepatitis delta virus and a T7 terminator was prepared. To...

journal_title：Journal of virological methods

authors： Myung J,Khalap N,Kalkeri G,Garry R,Dash S

更新日期：2001-05-01 00:00:00

abstract：:Arboviruses in the genus Flavivirus are major causes of human disease worldwide, and yet they are widely under reported. This is partially due to the classical methods of detection, using virologic or immunologic techniques, which typically lack sensitivity and specificity or require biological containment facilities ...

journal_title：Journal of virological methods

authors： Dyer J,Chisenhall DM,Mores CN

更新日期：2007-10-01 00:00:00

abstract：:Introduction of amino acids substitutions in murine leukemia virus genome is a powerful method to determine the relative importance of various viral factors in pathogenesis. However, introduction of such amino acids substitution could result in viruses at a selective disadvantage, and eventual selection of revertants....

journal_title：Journal of virological methods

authors： Danis C,Rassart E,Lemay G

更新日期：2005-05-01 00:00:00

abstract：:The appearance of cytomegalovirus (CMV) proteins in infected fibroblasts was determined by flow cytometry. The sequential production of immediate early (IE), early (E), and late (L) proteins reacting with respective monoclonal antibodies (mAbs) E13, 58/5, and 24/4 was determined in fibroblasts infected with the AD-169...

journal_title：Journal of virological methods

authors： Torigoe S,Campbell DE,Torigoe F,Michelson S,Starr SE

更新日期：1993-12-15 00:00:00

abstract：:A transfer vector was constructed in which the neomycin resistance (neo) gene was under the control of a copy of Autographa californica nuclear polyhedrosis virus (AcMNPV) IE1 gene promoter at the p10 locus. After cotransfection of Spodoptera frugiperda (Sf9) cells with the transfer vector and infectious AcMNPV DNA, t...

journal_title：Journal of virological methods

authors： Zhu FX,Qi YP,Huang YX,Bing Q

更新日期：1996-10-01 00:00:00

abstract：:Conventional method to purify/concentrate dengue virus (DENV) is time-consuming with low virus recovery yield. Herein, we applied cellufine sulfate column chromatography to purify/concentrate DENV based on the mimicry between heparan sulfate and DENV envelope protein. Comparative analysis demonstrated that this new me...

journal_title：Journal of virological methods

authors： Kanlaya R,Thongboonkerd V

更新日期：2016-08-01 00:00:00

abstract：:Systematic solid-phase synthesis of all possible overlapping nonapeptides of the 1381 amino acid sequence of the Epstein-Barr virus major capsid protein (EBV-MCP) was used to identify the position of linear antigen epitopes on this protein as recognised by human polyclonal antisera. Antisera were selected for reactivi...

journal_title：Journal of virological methods

authors： Middeldorp JM,Meloen RH

更新日期：1988-09-01 00:00:00

abstract：:A lateral flow device (LFD) for the detection of swine vesicular disease (SVD) virus (SVDV) and differential diagnosis from foot-and-mouth disease (FMD) was developed using a monoclonal antibody (Mab C70). The performance of the LFD was evaluated in the laboratory on suspensions of vesicular epithelia and cell culture...

journal_title：Journal of virological methods

authors： Ferris NP,Nordengrahn A,Hutchings GH,Paton DJ,Kristersson T,Merza M

更新日期：2010-02-01 00:00:00

abstract：:Five methods for the RNA detection of rabies virus were directly compared in this study. These included conventional nucleic acid sequence-based amplification with electrochemiluminescence (NASBA-ECL) assay, reverse transcription (RT)-heminested (hn) polymerase chain reaction (PCR) and TaqMan real-time RT-PCR using pr...

journal_title：Journal of virological methods

authors： Wacharapluesadee S,Phumesin P,Supavonwong P,Khawplod P,Intarut N,Hemachudha T

更新日期：2011-08-01 00:00:00

abstract：:The latent membrane protein 1 (LMP1) of Epstein-Barr virus (EBV) is one of two postulated viral oncogenic proteins. Sequence variations, and in particular a 30 base pair deletion variant called CAO, may define different disease populations. We developed a panel of rat monoclonal antibodies (MAb) specific for the non-w...

journal_title：Journal of virological methods

authors： Nicholls J,Hahn P,Kremmer E,Fröhlich T,Arnold GJ,Sham J,Kwong D,Grässer FA

更新日期：2004-03-01 00:00:00

abstract：:The introduction of molecular diagnostic methods for investigation of gastroenteritis has significantly reduced the diagnostic gap. However, approximately 25% of cases of gastroenteritis remain undiagnosed even after screening for bacteria, parasites and viruses using the most sensitive PCR and RT-PCR methods availabl...

journal_title：Journal of virological methods

authors： Braham S,Iturriza-Gómara M,Gray J

更新日期：2009-06-01 00:00:00

abstract：:A simple immunoperoxidase assay (IPA), adapted for detection of serum IgM antibodies to cytomegalovirus (CMV) is described. The antigen consisted of CMV infected human embryonic fibroblasts or isolated nuclei. The sera were absorbed with aggregated gamma-globulins prior to testing. Rabbit anti-human IgM peroxidase con...

journal_title：Journal of virological methods

authors： Singer Y,Kimmel N,Sarov I

更新日期：1985-05-01 00:00:00

abstract：:The laboratory diagnosis of hepatitis B virus (HBV) infection is based mainly on serological assays. Yet the detection and quantitation of viral DNA is necessary when addressing directly the question of infectivity or when monitoring the viral load during therapy. Standard hybridization assays allow for exact quantita...

journal_title：Journal of virological methods

authors： Weinberger KM,Wiedenmann E,Böhm S,Jilg W

更新日期：2000-03-01 00:00:00

abstract：:Research progress on the pleiotropic effects of interferons (IFN) has thus far required detecting responses by weak IFN signals. The activity of 2',5'-oligoadenylate synthetase (2-5OAS) is a valuable indicator in the prognosis and IFN treatment of patients with viral diseases such as hepatitis B and C. Although serum ...

journal_title：Journal of virological methods

authors： Uno K,Suginoshita Y,Kakimi K,Moriyasu Y,Nakano K,Nakamura N,Fujita T,Horino Y,Sato T,Kishida T

更新日期：2006-09-01 00:00:00

abstract：:The human immunodeficiency virus type 1 (HIV-1) gp41 plays an important role in membrane fusion between viruses and target cells. The gp41 ectodomain contains two heptad repeat regions adjacent to the N and C-termini. Peptides derived from these two regions, designated N and C-peptides, are potent inhibitors of HIV-1 ...

journal_title：Journal of virological methods

authors： Jiang S,Lin K,Zhang L,Debnath AK

更新日期：1999-06-01 00:00:00

abstract：:Emergence of lamivudine (LAM) resistance causes treatment failure in patients with chronic hepatitis B and compromise the efficacy of subsequent salvage therapies with other nucleot(s)ide analogs (NAs). Establishment of cell-based assays supporting LAM-resistant hepatitis B virus (HBV) replication will not only provid...

journal_title：Journal of virological methods

authors： Zhang Y,Zhang Y,Kang Y,Wang J,Liu H,Zhu H,Qin Y,Mao R,Lin X,Lu M,Zhang J

更新日期：2014-06-01 00:00:00