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A novel virus-encoded nucleocytoplasmic shuttling protein: the UL3 protein of herpes simplex virus type 1.

Abstract：

:Herpes simplex virus type 1 (HSV-1) UL3 protein is a nuclear protein. In this study, the molecular mechanism of the subcellular localization of UL3 was characterized by fluorescence microscopy in living cells. A nuclear localization signal (NLS) and a nuclear export signal (NES) were also identified. UL3 was demonstrated to target to the cytoplasm through the NES via chromosomal region maintenance 1 (CRM-1) dependent pathway, and to the nucleus through RanGTP-dependent mechanism. Heterokaryon assays confirmed that UL3 was capable of shuttling between the nucleus and the cytoplasm. These results demonstrate that the UL3 protein is a novel HSV-1 encoded nucleocytoplasmic shuttling protein.

journal_name

J Virol Methods

journal_title

Journal of virological methods

authors

Zheng C,Lin F,Wang S,Xing J

doi

10.1016/j.jviromet.2011.08.004

subject

Has Abstract

pub_date

2011-11-01 00:00:00

pages

206-10

issue

2

eissn

0166-0934

issn

1879-0984

pii

S0166-0934(11)00322-3

journal_volume

177

pub_type

杂志文章

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