Abstract：
:Based on variation in nucleotide sequence of 5'-untranslated region, GB virus type C (GBV-C) and hepatitis G virus (HGV) can be classified into three major genotypes. In addition to this classification, a novel genotype of GBV-C/HGV was identified and designated type 4. However, genotyping of GBV-C/HGV has been hampered by the lack of suitable assays. In this study, a simple and precise genotyping system based on PCR using the type-specific primers was developed for the determination of genotypes 1, 2, 3, and 4 of GBV-C/HGV. A total of 235 serum samples obtained from 11 different countries were tested by our PCR genotyping system of GBV-C/HGV. The results revealed that type 1 was prevalent mainly in Ghana, type 2 was prevalent in the USA, Spain, Egypt, Nepal and Myanmar, type 3 was prevalent in Japan and Bolivia, and type 4 was prevalent in Vietnam and Myanmar among the countries investigated in the present study. To confirm the specificity of the results of genotyping by PCR, phylogenetic analysis in the 5'-untranslated region of GBV-C/HGV was undertaken in 99 serum samples. By this analysis, the specificity of the genotyping system by PCR was confirmed. This assay system may be useful for rapid typing of GBV-C/HGV RNA when either epidemiological or transmission studies of this agent are carried out.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Naito H,Abe Kdoi
10.1016/s0166-0934(00)00207-xsubject
Has Abstractpub_date
2001-01-01 00:00:00pages
3-9issue
1eissn
0166-0934issn
1879-0984pii
S0166-0934(00)00207-Xjournal_volume
91pub_type
杂志文章abstract：:The use of adenovirus type 35 (Ad35) as a vector in vaccine and gene therapy studies is promising due to its broad cell tropism and low seroprevalence in humans. However, to date, a simple and effective system for producing recombinant Ad35 (rAd35) has not been well developed. This report describes a two-plasmid Ad35-...
journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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pub_type： 杂志文章
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journal_title：Journal of virological methods
pub_type： 杂志文章
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
pub_type： 杂志文章
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journal_title：Journal of virological methods
pub_type： 杂志文章
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更新日期：2014-06-01 00:00:00
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pub_type： 杂志文章
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更新日期：1984-05-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2014.01.003
更新日期：2014-04-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/0166-0934(95)01956-1
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journal_title：Journal of virological methods
pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Journal of virological methods
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更新日期：2009-07-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/0166-0934(94)90183-x
更新日期：1994-12-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2010.11.014
更新日期：2011-01-01 00:00:00
abstract：:Inflammasome plays a major role in innate immune responses by activating caspase-1, resulting in secretion of interleukin-1β (IL-1β) and inflammatory pathologic responses. IL-1β release is widely used as an indirect readout to study inflammasome activation. Here we report an iGLuc reporter (pro-IL-1β-Gluc) of pig orig...
journal_title：Journal of virological methods
pub_type： 杂志文章
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更新日期：2020-11-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/0166-0934(88)90062-6
更新日期：1988-09-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/0166-0934(87)90084-x
更新日期：1987-12-01 00:00:00
abstract：:A real-time PCR assay, which enables simultaneous detection and differentiation of all three serotypes of Marek's disease virus, without the need for post-PCR sequencing, has been developed. The assay is based on the primer-probe energy transfer real-time PCR, which has a relatively high tolerance towards point mutati...
journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2009.12.012
更新日期：2010-04-01 00:00:00
abstract：:TaqMan Mismatch Amplification Mutation Assay (TaqMAMA) is a highly sensitive allelic discrimination method. The mismatch amplification mutation assay (MAMA) is based on preferential amplification of mutant allele by the 'MAMA' primer, which is designed to have two mismatches with the wild-type allele and only one mism...
journal_title：Journal of virological methods
pub_type： 杂志文章,随机对照试验
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更新日期：2008-11-01 00:00:00
