Abstract：
:Recently, sequences from a putative member of the Flaviviridae, GB virus C (GBV-C), were isolated from the serum of patients with cryptogenic hepatitis. These sequences were 83-99% identical at the nucleotide level. Because of the divergence between these GBV-C isolates, it is likely that the PCR-based detection assay yields false negatives, underestimating dramatically the true prevalence of GBV-C in human hepatitis. We report the design of a GBV-C consensus oligonucleotide primer pair that is superior to those originally described. These primers identify GBV-C sequences in cases of cryptogenic hepatitis, allowing a better estimation of the prevalence of this virus in human populations.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Leary TP,Muerhoff AS,Simons JN,Pilot-Matias TJ,Erker JC,Chalmers ML,Schlauder GG,Dawson GJ,Desai SM,Mushahwar IKdoi
10.1016/0166-0934(95)01956-1subject
Has Abstractpub_date
1996-01-01 00:00:00pages
119-21issue
1eissn
0166-0934issn
1879-0984pii
0166093495019561journal_volume
56pub_type
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