Abstract:
:Specific cell-surface binding is the essential first step for cellular invasion by viruses. To understand this process, various methods to evaluate binding properties of viruses to cells have been developed. However, many rely on radioactive labeling or indirect immunofluorescence. The development of a novel fluorescence binding assay for poliovirus is described. Poliovirus (type 1 Mahoney or Sabin) was labeled directly with fluorescein using a commercially available fluoresceination kit. Fluorescently labeled poliovirus was bound to its specific receptor on Hela or U937 cells and detected by flow cytometric analysis. Specific binding and infectivity was retained, although reduced, depending on the extent of fluoresceination. Therefore, depending on the users' requirements, the extent of fluoresceination must be titrated carefully to achieve maximal fluorescence and minimal functional destruction. It is likely that this method may be useful with other viruses.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Freistadt MS,Eberle KEdoi
10.1016/j.jviromet.2005.08.011subject
Has Abstractpub_date
2006-06-01 00:00:00pages
1-7issue
1-2eissn
0166-0934issn
1879-0984pii
S0166-0934(05)00272-7journal_volume
134pub_type
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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