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Preparation of noninfectious hepatitis A virus hemagglutinin for detecting hemagglutination inhibition antibodies.

Abstract：

:Hepatitis A virus (HAV) harvested from infected MRC-5 cells can hemagglutinate various species of erythrocytes at acid pH (Eckels et al., 1989). Further studies revealed that the majority of the hemagglutinin (HA) in MRC-5 and BS-C-1 cells was cell-associated. A simplified procedure for preparing HAV-HA consisted of collecting infected cells in phosphate-buffered saline followed by three cycles of freeze-thawing and sonication. The fluids were clarified and stored at 4 degrees C. The analysis of HA by rate-zonal sucrose gradient centrifugation indicated that the majority of HA co-migrated with infectious virus. Complete inactivation of infectious HAV with 0.03% beta-propiolactone (BPL) did not affect HA activity, while inactivation with 0.05% formalin caused a 16-fold reduction in titer. There was no difference in HAI antibody titers when BPL-treated HA was compared to untreated HA in the hemagglutination inhibition (HAI) test.

journal_name

J Virol Methods

journal_title

Journal of virological methods

authors

Dubois DR,Binn LN,Summers PL,Timchak RL,Barvir DA,Marchwicki RH,Eckels KH

doi

10.1016/0166-0934(90)90123-w

subject

Has Abstract

pub_date

1990-06-01 00:00:00

pages

299-304

issue

3

eissn

0166-0934

issn

1879-0984

pii

0166-0934(90)90123-W

journal_volume

28

pub_type

杂志文章

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