Abstract：
:The large double-stranded DNA (ds DNA) viruses were among the first to be used to construct recombinant viruses, but to date this has not been achieved with any members of the ds DNA virus family, Iridoviridae. We identified a non-essential gene, the viral homologue of eukaryotic initiation factor 2alpha (eIF-2alpha), in Bohle iridovirus (BIV, genus Ranavirus). A recombinant BIV was constructed with the neomycin resistance gene and the Bufo marinus (cane toad) adult globin gene inserted into the BIV eIF-2alpha region. Adult globin expressed by the virus was detected on western blot, demonstrating that foreign genes can be expressed by the recombinant BIV in vitro and suggesting the possibility of using a recombinant BIV in the biological control of cane toads.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Pallister J,Goldie S,Coupar B,Shiell B,Michalski WP,Siddon N,Hyatt Adoi
10.1016/j.jviromet.2007.08.016subject
Has Abstractpub_date
2007-12-01 00:00:00pages
419-23issue
1-2eissn
0166-0934issn
1879-0984pii
S0166-0934(07)00329-1journal_volume
146pub_type
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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abstract：:HTLV-1 and HTLV-2 are highly related delta-retroviruses that infect and transform T-lymphocytes, but have distinct pathogenic properties. HTLV replication and survival requires the expression of multiple gene products from an unspliced and a series of highly related alternatively spliced mRNA species. To date, the com...
journal_title：Journal of virological methods
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doi：10.1016/j.jviromet.2007.01.023
更新日期：2007-06-01 00:00:00
