Real-time TaqMan RT-PCR for detection of maize chlorotic mottle virus in maize seeds.

Abstract:

:Maize chlorotic mottle virus (MCMV) causes corn lethal necrosis disease, and can be transmitted through infected maize seeds. It remains a challenge to detect this virus in the seeds to prevent its introduction and infection. For this purpose, a real-time TaqMan RT-PCR procedure for efficient detection of MCMV was developed. The sensitivity of the method was 4 fg of total RNA or 25 copies of RNA transcripts, which was approximately ten-fold higher than conventional RT-PCR gel electrophoresis method. The successful detection of MCMV in maize seeds suggested the feasibility of this procedure for routine testing.

journal_name

J Virol Methods

authors

Zhang Y,Zhao W,Li M,Chen H,Zhu S,Fan Z

doi

10.1016/j.jviromet.2010.11.002

subject

Has Abstract

pub_date

2011-01-01 00:00:00

pages

292-4

issue

1

eissn

0166-0934

issn

1879-0984

pii

S0166-0934(10)00400-3

journal_volume

171

pub_type

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