Abstract:
:Maize chlorotic mottle virus (MCMV) causes corn lethal necrosis disease, and can be transmitted through infected maize seeds. It remains a challenge to detect this virus in the seeds to prevent its introduction and infection. For this purpose, a real-time TaqMan RT-PCR procedure for efficient detection of MCMV was developed. The sensitivity of the method was 4 fg of total RNA or 25 copies of RNA transcripts, which was approximately ten-fold higher than conventional RT-PCR gel electrophoresis method. The successful detection of MCMV in maize seeds suggested the feasibility of this procedure for routine testing.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Zhang Y,Zhao W,Li M,Chen H,Zhu S,Fan Zdoi
10.1016/j.jviromet.2010.11.002subject
Has Abstractpub_date
2011-01-01 00:00:00pages
292-4issue
1eissn
0166-0934issn
1879-0984pii
S0166-0934(10)00400-3journal_volume
171pub_type
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