Abstract:
:Group A rotaviruses (RVs) are important pathogens that cause acute, dehydrating gastroenteritis in infants and young children. In this study, a multiplex real-time polymerase chain reaction protocol using primers and TaqMan(®) probes specific for viral VP4 and VP7 genes was evaluated. This assay offers simultaneous genotyping and quantification of the most common RV genotypes G1P[8], G2P[4], G3P[8], G4P[8], and G9P[8]. It was compared to the molecular typing results provided by conventional PCR. A total of 92 archived stool specimens obtained from children younger than 5 years old with the diagnosis of acute gastroenteritis were examined. Real-time PCR assay detected rotavirus strains among the most common genotype combinations G4P[8] (70.7%), G1P[8] (10.9%), G2P[4] (5.4%), G9P[8] (2.2%). This new assay described has an acceptable sensitivity (low limit 6.3×10(2)copies/g of stool).
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Kottaridi C,Spathis AT,Ntova CK,Papaevangelou V,Karakitsos Pdoi
10.1016/j.jviromet.2011.12.009subject
Has Abstractpub_date
2012-03-01 00:00:00pages
49-53issue
1-2eissn
0166-0934issn
1879-0984pii
S0166-0934(11)00494-0journal_volume
180pub_type
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