Abstract：
:Rubella virosomes were prepared from performed liposomes and detergent solubilized viral hemagglutinin. The liposomes were made from lecithin/dicetyl phosphate (3.5 : 1) films resuspended in NTE buffer and sonicated. Viral hemagglutinin was prepared from purified virus after solubilization with Triton X-100 and centrifugation through a sucrose gradient containing beta-D-octylglucoside. Electron microscopy of the rate zonal purified virosomes, showed virus-like structures of 40 - 80 nm. The virosomes retained the biological activity of the hemagglutinin and had a buoyant density of 1.2 g/cm3.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Trudel M,Marchessault F,Payment Pdoi
10.1016/0166-0934(81)90069-0subject
Has Abstractpub_date
1981-11-01 00:00:00pages
187-92issue
4eissn
0166-0934issn
1879-0984pii
0166-0934(81)90069-0journal_volume
3pub_type
杂志文章abstract：:A rapid and effective ELISA for measuring Epstein-Barr virus (EBV)-neutralizing antibodies in human sera was devised to replace the existing cumbersome method involving the inhibition of fetal cord blood B-cell transformation by the virus. The new method will be invaluable for assessing antibody responses in human sub...
journal_title：Journal of virological methods
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abstract：:Rabies is an infectious viral disease, characterized as a neglected zoonosis, responsible for nearly 60,000 deaths annually. The virus is transmitted mainly by dogs in Africa and Asia, and wildlife in Europe and the Americas, to all mammals' species, causing severe encephalitis almost always fatal after the onset of n...
journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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更新日期：1991-11-01 00:00:00
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更新日期：1994-05-01 00:00:00
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
pub_type： 杂志文章
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journal_title：Journal of virological methods
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更新日期：1982-11-01 00:00:00
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更新日期：2013-03-01 00:00:00
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journal_title：Journal of virological methods
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doi：10.1016/0166-0934(87)90084-x
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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pub_type： 杂志文章
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更新日期：1997-11-01 00:00:00
abstract：:The occurrence of virus belonging to the putative genus Influenzavirus D, has been demonstrated all-around the world arousing interest within the scientific community. Most of the published virological surveys are based on the first described Real-Time PCR method, designed on the PB1 gene of the first isolate. The nec...
journal_title：Journal of virological methods
pub_type： 杂志文章
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更新日期：2017-05-01 00:00:00
abstract：:A reverse-transcription competitive PCR (RT-cPCR) combined with chemiluminescence hybridization was designed for the detection and quantitative determination of serum hepatitis C virus (HCV) RNA. The concentration of HCV RNA was calculated based on an external standard curve that was generated by coamplification of in...
journal_title：Journal of virological methods
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更新日期：2002-05-01 00:00:00
