Abstract:
:A rapid and effective ELISA for measuring Epstein-Barr virus (EBV)-neutralizing antibodies in human sera was devised to replace the existing cumbersome method involving the inhibition of fetal cord blood B-cell transformation by the virus. The new method will be invaluable for assessing antibody responses in human subjects participating in EBV gp340 vaccine trials. The ELISA developed uses the human serum antibody to be tested to inhibit standardised binding of an EBV-neutralizing monoclonal antibody (mAb) to gp340 itself or its recombinant derivatives. A serum which has high EBV-neutralizing antibody titres inhibits the binding of neutralizing mAb to gp340 more than a serum with low levels. EBV neutralisation antibody titres obtained by the new inhibition ELISA correlate well with values obtained using the lengthy conventional assay where inhibition of B-cell transformation is assessed. The new assay can be carried out in a few hours compared to 4-5 weeks for the conventional test and could be automated for processing very large sample numbers in vaccine trials.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Wilson AD,Morgan AJdoi
10.1016/s0166-0934(98)00054-8subject
Has Abstractpub_date
1998-07-01 00:00:00pages
11-9issue
1eissn
0166-0934issn
1879-0984pii
S0166-0934(98)00054-8journal_volume
73pub_type
杂志文章abstract::The amplification of the 468 bp fragment of the BHV-1 genome by PCR is described. The 22 bp oligomers from the BHV-1 gI gene were used as primers. For successful amplification the thermal denaturation (100 degrees C/8 min, ice) of the DNA sample was carried out prior to the cycling (95 degrees C for 1 min, 56 degrees ...
journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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更新日期:2012-02-01 00:00:00
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journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/s0166-0934(97)00179-1
更新日期:1998-02-01 00:00:00
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journal_title:Journal of virological methods
pub_type: 杂志文章
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更新日期:2021-02-01 00:00:00
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journal_title:Journal of virological methods
pub_type: 杂志文章
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更新日期:2007-12-01 00:00:00
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journal_title:Journal of virological methods
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更新日期:1991-05-01 00:00:00
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journal_title:Journal of virological methods
pub_type: 杂志文章
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更新日期:2018-05-01 00:00:00
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journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/0166-0934(84)90040-5
更新日期:1984-02-01 00:00:00
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journal_title:Journal of virological methods
pub_type: 杂志文章
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更新日期:2014-01-01 00:00:00
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journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/j.jviromet.2010.06.013
更新日期:2010-10-01 00:00:00
abstract::In preparation for field trials, a nuclear polyhedrosis virus (NPV) of the pine beauty moth, Panolis flammea, was mass produced in vivo in an alternative (heterologous) host, the cabbage moth, Mamestra brassicae. Simultaneously, Mamestra NPV was also produced in M. brassicae. This homologous NPV/host system was a cons...
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pub_type: 杂志文章
doi:10.1016/0166-0934(88)90019-5
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journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/j.jviromet.2011.11.024
更新日期:2012-02-01 00:00:00
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journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/s0166-0934(97)00208-5
更新日期:1998-06-01 00:00:00
abstract::Water is one major pathways by which the white spot syndrome virus (WSSV) pathogen enters aquaculture facilities. This paper describes the production and use of a capacitive biosensor for the quantitative detection of as little as 1copy/μl of WSSV in shrimp pond water. A glutathione-S-transferase tag for white spot bi...
journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/j.jviromet.2011.01.010
更新日期:2011-04-01 00:00:00
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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doi:10.1016/j.jviromet.2009.04.035
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abstract::Human papillomavirus (HPV) infection is the primary cause of cervical cancer. The Quantivirus(®) HPV E6/E7 RNA 3.0 assay (DiaCarta, CA, USA) detects E6/E7 mRNA of 13 high risk subtypes and 6 low risk subtypes. Cervical specimens collected in PreservCyt were processed for HPV detection. Cervical biopsies were taken onl...
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journal_title:Journal of virological methods
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abstract::With the approval of the first HIV-1 integrase inhibitor raltegravir and a second one in phase III clinical development (elvitegravir), genotypic and phenotypic resistance assays are required to guide antiretroviral therapy and to investigate treatment failure. In this study, a genotypic and phenotypic recombinant vir...
journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/j.jviromet.2009.06.015
更新日期:2009-11-01 00:00:00
abstract::Formalinized chick erythrocytes agglutinated with Japanese encephalitis virus antigens over a wider range of pH (6.0-7.2) than fresh erythrocytes (pH 6.4-6.8). The virus antigens were unstable below pH 7.0 and therefore the use of the formalinized erythrocytes made it possible for a one-diluent system of the hemagglut...
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pub_type: 杂志文章
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更新日期:1980-01-01 00:00:00