当前位置： SCI文献检索 > JOURNAL OF VIROLOGICAL METHODS期刊下所有文献 > In vivo mass production in the cabbage moth (Mamestra brassicae) of a heterologous (Panolis) and a homologous (Mamestra) nuclear polyhedrosis virus.

In vivo mass production in the cabbage moth (Mamestra brassicae) of a heterologous (Panolis) and a homologous (Mamestra) nuclear polyhedrosis virus.

Abstract：

:In preparation for field trials, a nuclear polyhedrosis virus (NPV) of the pine beauty moth, Panolis flammea, was mass produced in vivo in an alternative (heterologous) host, the cabbage moth, Mamestra brassicae. Simultaneously, Mamestra NPV was also produced in M. brassicae. This homologous NPV/host system was a consistently more efficient production system than the heterologous one. Evidence is presented that increased humidity may improve levels of infection and, therefore, yield achieved. Full purification of the resultant product through a sucrose gradient resulted in a loss of virus of about 30%.

journal_name

J Virol Methods

journal_title

Journal of virological methods

authors

Kelly PM,Entwistle PF

doi

10.1016/0166-0934(88)90019-5

subject

Has Abstract

pub_date

1988-03-01 00:00:00

pages

249-56

issue

3-4

eissn

0166-0934

issn

1879-0984

pii

0166-0934(88)90019-5

journal_volume

19

pub_type

杂志文章

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