Detection of the bovine herpesvirus-1 (BHV-1) genome by PCR.

Abstract:

:The amplification of the 468 bp fragment of the BHV-1 genome by PCR is described. The 22 bp oligomers from the BHV-1 gI gene were used as primers. For successful amplification the thermal denaturation (100 degrees C/8 min, ice) of the DNA sample was carried out prior to the cycling (95 degrees C for 1 min, 56 degrees C for 1 min, 73 degrees C for 1 min). The presence of glycerol markedly enhances the PCR.

journal_name

J Virol Methods

authors

Vilcek S

doi

10.1016/0166-0934(93)90132-b

subject

Has Abstract

pub_date

1993-02-01 00:00:00

pages

245-7

issue

2

eissn

0166-0934

issn

1879-0984

pii

0166-0934(93)90132-B

journal_volume

41

pub_type

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