Abstract：
:Commercial enzyme-linked immunosorbent assay (ELISA) kits for the determination of the in vitro potency of recombinant hepatitis B vaccines, which detect hepatitis B surface antigen (HBsAg), have been used frequently as an alternative for traditional in vivo potency tests. With the constant need for validation procedures, an ELISA that could be employed to determine the in vitro potency of five recombinant hepatitis B vaccines simultaneously was established using two monoclonal antibodies. The use of two monoclonal antibodies produced "in house" specific for the small envelope protein S of the hepatitis B virus (HBV) resulted in the production of a highly specific, sensitive and stable ELISA. The standard ELISA parameters used in this study, considering the HBsAg content of each recombinant hepatitis B vaccine evaluated, resulted in a standard curve that could be applied for potency evaluations of different, commercial hepatitis B vaccine lots.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Costa CI,Delgado IF,da Costa JA,de Carvalho RF,Mouta Sda S Jr,Vianna CO,de Moraes MTdoi
10.1016/j.jviromet.2010.12.010subject
Has Abstractpub_date
2011-03-01 00:00:00pages
32-7issue
1-2eissn
0166-0934issn
1879-0984pii
S0166-0934(10)00451-9journal_volume
172pub_type
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