Abstract：
:Four commercial test kits for parvovirus B19 IgM were evaluated by testing 491 sera assembled into 7 panels. The serum panels were designed to assess sensitivity and specificity of the commercial assays and to reflect the various clinical settings in which acute B19 infection forms part of the differential diagnosis. A mu-capture radioimmunoassay (MACRIA) was used as the reference test. With respect to MACRIA, the commercial B19 IgM assays showed an overall sensitivity of 70.1-84.1% and specificity of 92.2 to 97.4%. Assay performance varied in different clinical situations. In sera from adults with acute B19 arthropathy, all 4 assays were 100% sensitive, but in children with fifth disease, the sensitivity ranged from 44.1 to 88.6%. The sensitivity of all 4 assays was also low when testing samples collected more than 6 weeks after onset of symptoms and in women with B19-associated embryopathy. Specificity was greater than 97% in healthy blood donors, but varied from 70.9 to 83.3% in patients acutely infected with other viruses, including rubella. Although the IgM test kits here evaluated may be usefully introduced for B19 diagnosis in certain settings, knowledge of their limitations will be important when results have been interpreted.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Cohen BJ,Bates CMdoi
10.1016/0166-0934(95)00040-2subject
Has Abstractpub_date
1995-09-01 00:00:00pages
11-25issue
1eissn
0166-0934issn
1879-0984pii
0166093495000402journal_volume
55pub_type
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
pub_type： 杂志文章
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更新日期：2006-11-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
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更新日期：2001-05-01 00:00:00
abstract：:Lentiviral vectors (LVs) are promising delivery systems for gene therapy. To enhance the efficiency of target cell transduction by LVs, protocols often include the addition of culture additives. In this study, the cationic amphipathic peptide LAH4-L1 (KKALLAHALHLLALLALHLAHALKKA), a DNA transfection agent, was evaluate...
journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2013.02.005
更新日期：2013-05-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
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更新日期：2007-12-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/0166-0934(87)90004-8
更新日期：1987-06-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/0166-0934(94)90122-8
更新日期：1994-07-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/0166-0934(82)90055-6
更新日期：1982-08-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/0166-0934(94)90081-7
更新日期：1994-04-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2006.06.002
更新日期：2006-10-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2018.10.018
更新日期：2019-01-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/0166-0934(94)90043-4
更新日期：1994-09-01 00:00:00
abstract：:The Roche Amplicor PCR kit was used to detect HIV-1 DNA in UK patients of known serostatus. Four false-negative and/or equivocal results were obtained from patients who were known to be anti HIV seropositive (Tosswill et al., 1994). Cells from the blood of these patients were shown to contain HIV DNA after extraction,...
journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/0166-0934(94)00139-8
更新日期：1995-03-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2004.06.020
更新日期：2004-11-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2014.05.022
更新日期：2014-09-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/0166-0934(90)90047-j
更新日期：1990-10-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/0166-0934(96)02057-5
更新日期：1996-07-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2017.06.014
更新日期：2017-10-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2017.09.003
更新日期：2017-11-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/0166-0934(93)90031-l
更新日期：1993-05-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2013.08.037
更新日期：2014-01-01 00:00:00
abstract：:Quantitative real-time PCR is being widely used in the identification of plasma donations that contain high levels of parvovirus B19, to ensure their exclusion from start pools used in the manufacture of plasma derived medicinal products. In this study, the primers and probe of one such published assay, are examined f...
journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2006.09.002
更新日期：2007-01-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/s0166-0934(98)00099-8
更新日期：1998-10-01 00:00:00
abstract：:The major concern for severe acute respiratory syndrome (SARS), caused by the SARS-associated coronavirus (SARS-CoV), is the lack of diagnostic and therapeutic agents. Using a phage display technology in a chicken system, high-affinity monoclonal antibody fragments against the SARS-CoV spike protein were characterized...
journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2007.06.010
更新日期：2007-12-01 00:00:00
abstract：:Cervical carcinoma is one of the major consequences of human papillomavirus (HPV) infections. Although HPV infections of cervix do not always progress to cancer, 90% cases of cervical cancer have been found associated with high risk HPV (hrHPV) infection. Usually, HPV infection is asymptomatic; however, this asymptoma...
journal_title：Journal of virological methods
pub_type： 杂志文章,评审
doi：10.1016/j.jviromet.2015.06.015
更新日期：2015-09-15 00:00:00
abstract：:A method for co-amplification of multiple viral sequences of HIV-1 and HIV-2 by polymerase chain reaction was designed. The technique resulted in the specific detection of each type of virus and allowed the amplification of as few as two copies of target DNA. The amplification of multiple regions of the viral genome o...
journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/0166-0934(94)90053-1
更新日期：1994-08-01 00:00:00
abstract：:Reverse genetics system is a powerful tool to study the function of a particular gene. The currently available reverse genetics system for Novirhabdovirus is based on vaccinia-driven T7 RNA polymerase expression. An improved system for the recovery of infectious hematopoietic necrosis virus (IHNV) was developed which ...
journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2010.03.023
更新日期：2010-08-01 00:00:00
abstract：:Highly pathogenic avian influenza virus H5N1 is a continuous threat to public health and poultry industry. The recurrence of the H5N1 led us to develop a robust, specific, and rapid detection method for the virus. In this study, an impedance aptasensor was developed for the virus detection using specific H5N1 aptamer ...
journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2016.07.018
更新日期：2016-10-01 00:00:00
abstract：:A method for coupling cytomegalovirus (CMV) antigens to aldehyde-fixed erythrocytes for use in a passive hemagglutination assay for antibody to CMV is described. This method uses acid buffer instead of tannic acid for coupling CMV-complement fixation (CF) antigen to the stabilized cells. The coated cells are stable fo...
journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/0166-0934(83)90002-2
更新日期：1983-09-01 00:00:00
abstract：:Giant salamander iridovirus (GSIV) belongs to the epizootic genus Ranavirus, and is the cause of epidemic diseases associated with high mortality and great losses to artificial breeding and farming. Here, we established a simple, accurate, and reliable cross-priming amplification (CPA) method to detect GSIV. The CPA a...
journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2019.113678
更新日期：2019-12-01 00:00:00
