Abstract：
:Cyprinid herpesvirus-3 (CyHV-3) is an etiological agent of a notifiable disease that causes high mortality rates affecting both the common and koi carp Cyprinus carpio L. There is no current treatment strategy to save CyHV-3 infected fish. RNA mediated interference (RNAi) is an emerging strategy used for understanding gene function and is a promising method in developing novel therapeutics and antiviral medications. For this study, the possibility of activating the RNAi pathway by the use of small interfering (si)RNAs was tested to inhibit in vitro viral replication of CyHV-3 in common carp brain (CCB) cells. The siRNAs were designed to target either thymidine kinase (TK) or DNA polymerase (DP) genes, which both code for transcripts involved in DNA replication. The inhibition of viral replication caused by the siRNAs was measured by a reporter gene, termed ORF81. Treatment with siRNA targeting either TK or DP genes reduced the release of viral particles from infected CCB cells. However, siRNA targeting DP was most effective at reducing viral release as measured by qPCR.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Gotesman M,Soliman H,Besch R,El-Matbouli Mdoi
10.1016/j.jviromet.2014.05.022subject
Has Abstractpub_date
2014-09-01 00:00:00pages
63-6eissn
0166-0934issn
1879-0984pii
S0166-0934(14)00214-6journal_volume
206pub_type
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