Abstract:
:A novel reassortant influenza A (H7N9) virus emerged recently in China. In this study, a duplex real-time reverse transcription polymerase chain reaction (rRT-PCR) assay was developed for the simultaneous detection of hemagglutinin (HA) and neuraminidase (NA) genes of H7N9 influenza viruses. The sensitivity of the assay was determined to be 10 RNA copies per reaction for both HA and NA genes. No cross-reactivity was observed with other influenza virus subtypes or respiratory tract viruses. One hundred and forty-six clinical and environmental specimens were tested and compared with reference methods and were found to be consistent. The assay is suitable for large-scale screening due to short turnaround times and high specificity, sensitivity, and reproducibility.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Li Y,Wu T,Qi X,Ge Y,Guo X,Wu B,Yu H,Zhu Y,Shi Z,Wang H,Cui L,Zhou Mdoi
10.1016/j.jviromet.2013.08.021subject
Has Abstractpub_date
2013-12-01 00:00:00pages
194-6issue
1-2eissn
0166-0934issn
1879-0984pii
S0166-0934(13)00353-4journal_volume
194pub_type
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